Difference between revisions of "Team:VIT Vellore"

 
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{{VIT_Vellore}}
 
{{VIT_Vellore}}
 
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            <h1>toggle<span class="em">pH</span></h1>
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            <h2>VIT Vellore</h2>
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        <h1>toggle<span class="em-text">pH</span></h1>
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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/f/f8/T--VIT_Vellore--ill1.png" alt="Illustration 1"/>
 
         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/f/f8/T--VIT_Vellore--ill1.png" alt="Illustration 1"/>
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         <p class="ill-description">The three promoters are two CadA and one PnhaA. An increase in industrialization has led to an overall increase in the carbon footprint, the major component being CO<sub>2</sub> which in turn led to global warming. Among other ill effects of industrialization, the one that has garnered a lot of attention is what we call <span class="em-text">Ocean Acidification</span>. It is also known as "the other CO<sub>2</sub> problem" and that's where our engineered microbe jumps in.</p>
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         <p class="ill-description">We use a plasmid system consisting of a composite part with two promoters</p>
 
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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/1/13/T--VIT_Vellore--ill2.png" alt="Illustration 2"/>
 
         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/1/13/T--VIT_Vellore--ill2.png" alt="Illustration 2"/>
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         <p class="ill-description">The three promoters are two CadA and one PnhaA. An increase in industrialization has led to an overall increase in the carbon footprint, the major component being CO<sub>2</sub> which in turn led to global warming. Among other ill effects of industrialization, the one that has garnered a lot of attention is what we call <span class="em-text">Ocean Acidification</span>. It is also known as "the other CO<sub>2</sub> problem" and that's where our engineered microbe jumps in.</p>
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         <p class="ill-description">This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH</p>
 
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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/archive/7/77/20181207184102%21T--VIT_Vellore--ill3.png" alt="Illustration 3"/>
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         <p class="ill-description">The three promoters are two CadA and one PnhaA. An increase in industrialization has led to an overall increase in the carbon footprint, the major component being CO<sub>2</sub> which in turn led to global warming. Among other ill effects of industrialization, the one that has garnered a lot of attention is what we call <span class="em-text">Ocean Acidification</span>. It is also known as "the other CO<sub>2</sub> problem" and that's where our engineered microbe jumps in.</p>
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         <p class="ill-description">Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase</p>
 
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         <p class="ill-description">The three promoters are two CadA and one PnhaA. An increase in industrialization has led to an overall increase in the carbon footprint, the major component being CO<sub>2</sub> which in turn led to global warming. Among other ill effects of industrialization, the one that has garnered a lot of attention is what we call <span class="em-text">Ocean Acidification</span>. It is also known as "the other CO<sub>2</sub> problem" and that's where our engineered microbe jumps in.</p>
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         <p class="ill-description">LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced</p>
 
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        <p class="ill-description">recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured</p>
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Latest revision as of 04:26, 8 December 2018

togglepH

VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced

Illustration 4

recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured