Difference between revisions of "Team:VIT Vellore"

 
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{{VIT_Vellore}}
 
{{VIT_Vellore}}
 
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<html>
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<div class="column full_size" >
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<style>
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<h1> Welcome to iGEM 2018! </h1>
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body {
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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    overflow: hidden !important;
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<img src="http://placehold.it/1080x320/c4baba/e4dede"></div>
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        <source src="https://static.igem.org/mediawiki/2018/d/db/T--VIT_Vellore--wavesvideo.mp4" type="video/mp4">
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    </video>
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    <div class="col-12" id="home-container">
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        <img id="igem-vit-logo" src="https://static.igem.org/mediawiki/2018/d/d3/T--VIT_Vellore--iGEEM2018FINAL.png" alt="iGEM VIT"/>
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        <h1>toggle<span class="em-text">pH</span></h1>
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        <h2>VIT Vellore</h2>
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        <button id="enter-site">Check us out!</button>
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    </div>
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</div>
  
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<h3>About our project</h3>
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<div id="ill-nav">
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<p>Overall increase in industrialization has led to an overall increase in the carbon footprint, the major component being CO<sub>2</sub> which led to global warming, among other ill effects of industrialization, the one that has gained a lot of attraction is what we call as<b> Ocean Acidification </b> also known as "the other CO<sub>2</sub> problem" and that's where our engineered microbe jumps in.</p>
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        <a class="ill-link" id="ill-1-link" href="#">&#10102;</a>
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        <a class="ill-link" id="ill-2-link" href="#">&#10103;</a>
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        <img class="ill-med" src="https://static.igem.org/mediawiki/2018/f/f8/T--VIT_Vellore--ill1.png" alt="Illustration 1"/>
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        <p class="ill-description">We use a plasmid system consisting of a composite part with two promoters</p>
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    </div>
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    <div id="ill-2" class="horizontal ill-container">
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        <img class="ill-med" src="https://static.igem.org/mediawiki/2018/1/13/T--VIT_Vellore--ill2.png" alt="Illustration 2"/>
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        <p class="ill-description">This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH</p>
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    </div>
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    <div id="ill-3" class="horizontal ill-container">
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        <img class="ill-med" src="https://static.igem.org/mediawiki/2018/archive/7/77/20181207184102%21T--VIT_Vellore--ill3.png" alt="Illustration 3"/>
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        <p class="ill-description">Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase</p>
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    </div>
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    <div id="ill-4" class="horizontal ill-container">
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        <img class="ill-med" src="https://static.igem.org/mediawiki/2018/archive/a/a5/20181207181318%21T--VIT_Vellore--ill4.png" alt="Illustration 4"/>
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        <p class="ill-description">LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced</p>
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    </div>
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<div id="ill-5" class="horizontal ill-container">
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        <img class="ill-med" src="https://static.igem.org/mediawiki/2018/f/fa/T--VIT_Vellore--ill5.png" alt="Illustration 4"/>
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        <p class="ill-description">recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured</p>
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    </div>
  
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<p>Geology reports suggest that between 1751 to 1996 there has been an increase of H<sup>+</sup> ions of upto 30% apart from that the overall pH has been decreased from 8.25 to 8.14. Increasing acidity is directly linked to being having potentially harmful consequences for marine organisms, such as depressing metabolic rates and immune responses in some organisms, and causing in worst cases coral bleaching</p>
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<script type="text/javascript">
  
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<p>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;"If we continue emitting CO2 at the same rate, by 2100 ocean acidity will increase by about 150 percent, a rate that has not been experienced for at least 400,000 years."</p>
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<p>-United Kingdom Ocean Acidification Research Programme, 2015</p>
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<p>Our engineered vibrio will interact with it’s surrounding environment to give real-time H<sub>+</sub> data which is then used by a pH-sensitive NhaA and CadA promoters which give maximum expression at pH 8.5 and pH 7 respectfully after which the expression is downregulated. With this 1.5 pH window our biobricks are designed so that the carbonic anhydrase genes that bring about a reduction in ocean acidification, once the pH is stabilized, repressor proteins are expressed which block the carbonic anhydrase expression so that no more shuttle of H<sup>+</sup> thereby saving marine flora and fauna from any damage</p>
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Latest revision as of 04:26, 8 December 2018

togglepH

VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced

Illustration 4

recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured