Difference between revisions of "Team:VIT Vellore"

 
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         <h1>toggle<span class="em-text">pH</span></h1>
 
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         <h2>VIT Vellore</h2>
 
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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/f/f8/T--VIT_Vellore--ill1.png" alt="Illustration 1"/>
 
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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/7/77/T--VIT_Vellore--ill3.png" alt="Illustration 3"/>
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         <p class="ill-description">Repressor molecule LexA is produced in order to repress the expression of the biobrick at low pH</p>
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         <p class="ill-description">Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase</p>
 
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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/archive/a/a5/20181207181318%21T--VIT_Vellore--ill4.png" alt="Illustration 4"/>
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         <p class="ill-description">LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process</p>
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         <p class="ill-description">LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced</p>
 
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         <img class="ill-med" src="https://static.igem.org/mediawiki/2018/f/fa/T--VIT_Vellore--ill5.png" alt="Illustration 4"/>
 
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         <p class="ill-description">LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process</p>
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         <p class="ill-description">recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured</p>
 
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Latest revision as of 04:26, 8 December 2018

togglepH

VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced

Illustration 4

recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured