Difference between revisions of "Team:Jilin China/Attributions"

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background: url(https://static.igem.org/mediawiki/2018/f/f3/T--Jilin_China--Description--Banner.jpeg) no-repeat top left;
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<section class="s1">
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  <div class="title_con">
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  <p>DESCRIPTION</p>
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  <br />
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  <table>
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  <tr>
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    <td><a href="#pragraph_1" class="clickwave">Promoters</a></td>
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    <td><a href="#pragraph_2" class="clickwave">Library</a></td>
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<div class="column full_size">
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    <td><a href="#pragraph_3" class="clickwave">What are we doing</a></td>
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<h1>Attributions</h1>
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<p>This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.
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  </table>
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  <div class="title_nav"><h2>Description</h2></div>
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<div class="bodycontent" style="padding:0;">
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  <!--<section class="s0"></section>-->
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  <li><a href="#pragraph_1">Promoters</a></li>
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  <li><a href="#pragraph_2">Library</a></li>
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  <li><a href="#pragraph_3">What are we doing</a></li>
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  </ul>
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  <section class="s2">
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  <ul>
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    <li class="pragraph_1" id="pragraph_1">
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    <div>
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      <h2>Synthetic Promoters Construction</h2>
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      <p>With the development of synthetic biology, scientists have set a goal to construct de novo biobricks, parts and systems, while realize the strandardization  of biological system. De novo genetic biobricks are of great value in metabolic engineering and systems biology, especially. Promoters represent critical elements that can participate in gene expression and regulation, which play an important role in artificial systems. However, promoters from different sources have sophisticated regulation system, low universality, unpredictable expression intensity and lack of standardization, their applications in synthetic biology have been impeded. Fortunately, de novo promoters construction and identification have provided a brand new method to address this issue.</p>
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      <p>A prokaryotic promoter is made up of -35 region, -10 region, spacers, UP element as conservative regions and other non-conserved regions. According to current researches, a promoter is also considered as core promoter with its downstream sequence including 5’Untranslated Region (5’UTR). Strictly, promoters and ribosomes binding sites (RBS) are regulatory elements of transcription and translation respectively. Because of the short sequence of RBS, we connect synthetic RBS library to the library of synthetic core promoters, then a novel library of promoters can be constructed.</p>
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    </div>
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    </li>
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    <li class="pragraph_2" id="pragraph_2">
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    <div>
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      <h2>Temperature-induced Promoter Library Construction</h2>
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      <p>Compared to widely used chemically induced promoters, physically induced promoters don’t require special inducer, reducing cost while avoiding super-sensitivity of inducer concentration. Besides, physically induced promoters have special characteristics and a vast application prospect, and among all kinds of  physical factors, temperature has become our main concern this year with its vast prospect in fermentation engineering, drug targeting and other fields. As a result, our project, with a view to construct and characterize synthetic regulated Temperature-Sensitive promoter libraries for 2018 iGEM competition.</p>
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      <p>According to current researches, there are three different types of biomolecules responsive to temperature: DNA, RNA and proteins. Actually, RNA is playing a dominant role in temperature sensing especially in bacteria. 5’UTR of mRNA forms secondary structure, in order to block or expose SD （Shine-Dalgarno sequence，binding to ribosomes in  prokaryote cells）sequence. Compared to DNA and proteins, it is easier to reconstruct temperature-sensing RNA.</p>
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      <p>Our primary objective is to construct a temperature-sensitive promoter library supplying the iGEM registry with temperature-sensitive promoter parts collection. However, natural temperature-sensitive promoters, which are in a small number lacking in standardization, are regulated by many trans-acting factors, we try to design and construct de novo synthetic temperature-sensitive promoters with current researches.
 
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    </div>
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<h3> Bronze Medal Criterion #3</h3>
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    </li>
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<p> All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.
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Please see the <a href="https://2018.igem.org/Judging/Medals">Medals requirements page</a> for more details.</p>
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      <h2>What are we doing?</h2>
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</div>
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      <p>We construct our promoter library with the free combination of core promoter and 5＇UTR, then the expression efficiency is easily to be changed with the replacement of different core promoters and sequence changes of 5’UTR, realizing the bi-level regulation of both transcription and translation. Four synthetic standard regulated libraries have been constructed:</p>
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      <ul>
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      <li>(1) Heat-induced RNA thermosensors library, directional reconstructions to secondary structures of RNA thermometers;</li>
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      <li>(2) Heat-repressible RNA thermosensors library, directional reconstructions to anti-RNaseE cleavage site;</li>
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      <li>(3) Cold-induced RNA thermosensors library, directional reconstructions to mRNA pseudoknot of CspA;</li>
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      <li>(4) Cold-repressible RNA thermosensors library, directional reconstructions to RNase III binding site.</li>
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      </ul>
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<div class="column third_size">
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<h3> What should this page contain?</h3>
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    </li>
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<li>Clearly state what the team accomplished</li>
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<li>General Support</li>
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<li>Project support and advice</li>
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<li>Fundraising help and advice</li>
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<li>Lab support</li>
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<li>Difficult technique support</li>
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<li>Project advisor support</li>
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<li>Wiki support</li>
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<li>Presentation coaching</li>
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<li>Human Practices support</li>
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<li> Thanks and acknowledgements for all other people involved in helping make a successful iGEM team</li>
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<p>Tell us if your institution teaches an iGEM or synthetic biology class and when you started your project:</p>
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<li>Does your institution teach an iGEM or synthetic biology course?</li>
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<li>When did you start this course?</li>
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<li>Are the syllabus and course materials freely available online?</li>
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<li>When did you start your brainstorming?</li>
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<li>When did you start in the lab?</li>
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<li>When did you start working on  your project?</li>
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<h3>Inspiration</h3>
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<p>Take a look at what other teams have done:</p>
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<ul>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Team">2011 Imperial College London</a> (scroll to the bottom)</li>
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<li><a href="https://2014.igem.org/Team:Exeter/Attributions">2014 Exeter </a></li>
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<li><a href="https://2014.igem.org/Team:Melbourne/Attributions">2014 Melbourne </a></li>
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<li><a href="https://2014.igem.org/Team:Valencia_Biocampus/Attributions">2014 Valencia Biocampus</a></li>
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<h3> Why is this page needed? </h3>
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<p>The Attribution requirement helps the judges know what you did yourselves and what you had help with. We don't mind if you get help with difficult or complex techniques, but you must report what work your team did and what work was done by others.</p>
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<p>
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For example, you might choose to work with an animal model during your project. Working with animals requires getting a license and applying far in advance to conduct certain experiments in many countries. This is difficult to achieve during the course of a summer, but much easier if you can work with a postdoc or PI who has the right licenses.</p>
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<h3> Can we base our project on a previous one? </h3>
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<p>Yes! You can have a project based on a previous team, or based on someone else's idea, <b>as long as you state this fact very clearly and give credit for the original project.</b> </p>
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Revision as of 04:12, 14 August 2018

DESCRIPTION


Promoters Library What are we doing

Description

  • Synthetic Promoters Construction

    With the development of synthetic biology, scientists have set a goal to construct de novo biobricks, parts and systems, while realize the strandardization of biological system. De novo genetic biobricks are of great value in metabolic engineering and systems biology, especially. Promoters represent critical elements that can participate in gene expression and regulation, which play an important role in artificial systems. However, promoters from different sources have sophisticated regulation system, low universality, unpredictable expression intensity and lack of standardization, their applications in synthetic biology have been impeded. Fortunately, de novo promoters construction and identification have provided a brand new method to address this issue.

    A prokaryotic promoter is made up of -35 region, -10 region, spacers, UP element as conservative regions and other non-conserved regions. According to current researches, a promoter is also considered as core promoter with its downstream sequence including 5’Untranslated Region (5’UTR). Strictly, promoters and ribosomes binding sites (RBS) are regulatory elements of transcription and translation respectively. Because of the short sequence of RBS, we connect synthetic RBS library to the library of synthetic core promoters, then a novel library of promoters can be constructed.

  • Temperature-induced Promoter Library Construction

    Compared to widely used chemically induced promoters, physically induced promoters don’t require special inducer, reducing cost while avoiding super-sensitivity of inducer concentration. Besides, physically induced promoters have special characteristics and a vast application prospect, and among all kinds of physical factors, temperature has become our main concern this year with its vast prospect in fermentation engineering, drug targeting and other fields. As a result, our project, with a view to construct and characterize synthetic regulated Temperature-Sensitive promoter libraries for 2018 iGEM competition.

    According to current researches, there are three different types of biomolecules responsive to temperature: DNA, RNA and proteins. Actually, RNA is playing a dominant role in temperature sensing especially in bacteria. 5’UTR of mRNA forms secondary structure, in order to block or expose SD （Shine-Dalgarno sequence，binding to ribosomes in prokaryote cells）sequence. Compared to DNA and proteins, it is easier to reconstruct temperature-sensing RNA.

    Our primary objective is to construct a temperature-sensitive promoter library supplying the iGEM registry with temperature-sensitive promoter parts collection. However, natural temperature-sensitive promoters, which are in a small number lacking in standardization, are regulated by many trans-acting factors, we try to design and construct de novo synthetic temperature-sensitive promoters with current researches.

  • What are we doing?

    We construct our promoter library with the free combination of core promoter and 5＇UTR, then the expression efficiency is easily to be changed with the replacement of different core promoters and sequence changes of 5’UTR, realizing the bi-level regulation of both transcription and translation. Four synthetic standard regulated libraries have been constructed:

    • (1) Heat-induced RNA thermosensors library, directional reconstructions to secondary structures of RNA thermometers;
    • (2) Heat-repressible RNA thermosensors library, directional reconstructions to anti-RNaseE cleavage site;
    • (3) Cold-induced RNA thermosensors library, directional reconstructions to mRNA pseudoknot of CspA;
    • (4) Cold-repressible RNA thermosensors library, directional reconstructions to RNase III binding site.