Difference between revisions of "Team:IIT-Madras/Description"

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<h1>ADaPtat1on</h1>
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<p style="font-size: 5.5mm;">
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<span style="font-size: 7mm;"><strong>Motivations</strong></span>
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<li>Initial Idea of lignin degradation to produce higher valued vanillin.
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We look up the literature and we look at nature that can degrade the aromatic rings of Lignin. We needed an organism that could degrade aromatics.</li>
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<li>For popular organism like E.coli and L. lactis., we need to make competent cells for transformation. We looked up into literature and nature and found many ‘Naturally Competent’ microorganisms. However, most of them are pathogenic and known to cause harm to humans. Hence, you cannot work with it in Biosafety level 1 lab. For example: <em>Streptococcus pneumoniae</em> (Griffith's "pneumococcus"), <em>Neisseria gonorrhoeae</em>, <em>Bacillus subtilis</em> and <em>Haemophilus influenzae</em>.</li>
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<h1>Project Description</h1>
 
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    <h2>ADaPtat1on : Expanding Toolkit for Acetinobacter baylyi </h2>
 
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      <i>Acinetobacter baylyi</i> is a gram-negative, soil-dwelling, non-pathogenic, naturally
 
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      competent and nutritionally versatile organism, known especially for its ability
 
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      to degrade aromatic compounds. However, only a few tools are available for gene
 
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      manipulation in Synthetic Biology experiments. This year, we plan to expand this
 
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      toolkit for A. baylyi ADP1 by making synthetic promoter library along with codon
 
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      optimized fluorescent reporter proteins for A. baylyi to achieve better control
 
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      over expression rates. Codon usage table was also created by us using the genomic
 
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      data. This can potentially open up various new exciting synthetic biology
 
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      opportunities with this unexplored organism.
 
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    </p>
 
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    <h2>Sample Image</h2>
 
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    <br>
 
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    <img src="https://static.igem.org/mediawiki/2018/7/75/T--IIT-Madras--logotext.png">
 
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      Acinetobacter baylyi is a gram-negative, soil-dwelling, non-pathogenic, naturally
 
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      competent and nutritionally versatile organism, known especially for its ability
 
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      to degrade aromatic compounds. However, only a few tools are available for gene
 
−
      manipulation in Synthetic Biology experiments. This year, we plan to expand this
 
−
      toolkit for A. baylyi ADP1 by making synthetic promoter library along with codon
 
−
      optimized fluorescent reporter proteins for A. baylyi to achieve better control
 
−
      over expression rates. Codon usage table was also created by us using the genomic
 
−
      data. This can potentially open up various new exciting synthetic biology
 
−
      opportunities with this unexplored organism.
 
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    </p>
 
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Revision as of 06:33, 14 October 2018

iGEM Collaborations Page

☰

Team: IIT-Madras/ADaPtat1on

ADaPtat1on

Motivations

  1. Initial Idea of lignin degradation to produce higher valued vanillin. We look up the literature and we look at nature that can degrade the aromatic rings of Lignin. We needed an organism that could degrade aromatics.
  2. For popular organism like E.coli and L. lactis., we need to make competent cells for transformation. We looked up into literature and nature and found many ‘Naturally Competent’ microorganisms. However, most of them are pathogenic and known to cause harm to humans. Hence, you cannot work with it in Biosafety level 1 lab. For example: Streptococcus pneumoniae (Griffith's "pneumococcus"), Neisseria gonorrhoeae, Bacillus subtilis and Haemophilus influenzae.