Difference between revisions of "Team:GreatBay China"

Line 6: Line 6:
 
<!--[if (gte IE 9)|!(IE)]><!--><html lang="en"> <!--<![endif]-->
 
<!--[if (gte IE 9)|!(IE)]><!--><html lang="en"> <!--<![endif]-->
 
<head>
 
<head>
<!-- Basic Page Needs
+
  <!-- Basic Page Needs
 
   ================================================== -->
 
   ================================================== -->
 
<meta charset="utf-8">
 
<meta charset="utf-8">
<!--[if lt IE 9]>
+
  <!--[if lt IE 9]>
<script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
+
    <script src="http://html5shim.googlecode.com/svn/trunk/html5.js"></script>
<![endif]-->
+
  <![endif]-->
<!-- Mobile Specific Metas
+
  <!-- Mobile Specific Metas
 
   ================================================== -->
 
   ================================================== -->
 
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=0">
 
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=0">
Line 6,420: Line 6,420:
 
}
 
}
 
.row{
 
.row{
width:950px;
+
  width:950px;
 
}
 
}
 
@media (max-width: 767px) {
 
@media (max-width: 767px) {
Line 6,675: Line 6,675:
 
}
 
}
 
body a{
 
body a{
transition: 0.5s all;
+
  transition: 0.5s all;
-webkit-transition: 0.5s all;
+
  -webkit-transition: 0.5s all;
-o-transition: 0.5s all;
+
  -o-transition: 0.5s all;
-moz-transition: 0.5s all;
+
  -moz-transition: 0.5s all;
-ms-transition: 0.5s all;
+
  -ms-transition: 0.5s all;
 
}
 
}
  
  
 
h1,h2,h3,h4,h5,h6,label,p{
 
h1,h2,h3,h4,h5,h6,label,p{
margin:0;
+
  margin:0;  
 
}
 
}
 
.client-js{
 
.client-js{
margin:0;
+
  margin:0;
border:none;
+
  border:none;
 
}
 
}
 
#top_title,#sideMenu,#firstHeading,#logo_2018{  
 
#top_title,#sideMenu,#firstHeading,#logo_2018{  
display: none;  
+
  display: none;  
margin-bottom:0;
+
  margin-bottom:0;
 
}
 
}
  
 
#content{
 
#content{
width:100%;
+
  width:100%;
height:auto;
+
  height:auto;
 
     border: none;
 
     border: none;
z-index: -99999;
+
  z-index: -99999;
background-size:100%;
+
  background-size:100%;
padding:0 0 0 0;
+
  padding:0 0 0 0;
border:none;
+
  border:none;
margin-top:-12px;
+
  margin-top:-12px;
margin-left:0;
+
  margin-left:0;
 
}
 
}
 
html,body,#globalWrapper,#top_menu_14,#top_menu_inside,#top_title,#firstHeading,#logo_2018{
 
html,body,#globalWrapper,#top_menu_14,#top_menu_inside,#top_title,#firstHeading,#logo_2018{
margin-top:0;
+
  margin-top:0;
margin-left:0;
+
  margin-left:0;
margin-bottom:0;
+
  margin-bottom:0;
margin-right:0;
+
  margin-right:0;
padding-bottom:0
+
  padding-bottom:0
border:none;
+
  border:none;
 
}
 
}
  
 
html,body{
 
html,body{
font-family: Delius;
+
  font-family: Delius;
+
 
background:#fff2e6;
+
  background:#fff2e6;
font-size:90%;
+
  font-size:90%;
  
 
}
 
}
Line 6,727: Line 6,727:
 
}
 
}
 
h1,h2,h3,h4,h5,h6,p{
 
h1,h2,h3,h4,h5,h6,p{
line-height:150%;
+
  line-height:150%;
font-weight:500;
+
  font-weight:500;
 
}
 
}
  
Line 6,734: Line 6,734:
 
/* the entire div */
 
/* the entire div */
 
.top-nav{
 
.top-nav{
display:inline-block;
+
  display:inline-block;
width:200px;
+
  width:800px;
z-index:1;
+
  z-index:1;
background-image:url('https://static.igem.org/mediawiki/2018/5/5a/T--GreatBay_China--Menu.png');  
+
  background-image:url('https://static.igem.org/mediawiki/2018/5/5a/T--GreatBay_China--Menu.png');  
background-repeat:no-repeat;
+
  background-repeat:no-repeat;
background-position:center;
+
  background-position:center;
background-size:120px;
+
  background-size:120px;
position:fixed;
+
  position:fixed;
font-size:120%;
+
  font-size:120%;
top:75px;
+
  top:75px;
left:50px;
+
  left:50px;
padding-bottom:120px;
+
  padding-bottom:120px;
 
}
 
}
  
  
 
*{
 
*{
margin:0; padding:0
+
  margin:0; padding:0
 
}
 
}
 
.top-nav #top span{
 
.top-nav #top span{
min-height:1em;
+
  min-height:1em;
line-height:1em;
+
  line-height:1em;
 
}
 
}
 
.top-nav a,a:hover{
 
.top-nav a,a:hover{
text-decoration:none;color:#000;
+
  text-decoration:none;color:#000;
 
}
 
}
 
/* the menu position and color*/
 
/* the menu position and color*/
 
.top-nav ul{
 
.top-nav ul{
position:relative;
+
  position:relative;
top:65px;
+
  top:65px;
list-style:none;margin:0 auto;
+
  list-style:none;margin:0 auto;
width:90px;
+
  width:90px;
line-height:2;
+
  line-height:2;
text-align:center;
+
  text-align:center;
font-size:1em;  
+
  font-size:1em;  
padding-bottom:100px;
+
  padding-bottom:100px;
color:rgb(88,73,63);
+
  color:rgb(88,73,63);
 
}
 
}
 
/* the pull out menu */
 
/* the pull out menu */
 
.top-nav ul li{
 
.top-nav ul li{
position:relative;
+
  position:relative;
height:31px;
+
  height:31px;
_float:left;
+
  _float:left;
 
}
 
}
 
/* style for menu */
 
/* style for menu */
 
.top-nav ul li span,ul li div a{
 
.top-nav ul li span,ul li div a{
color:rgb(88,73,63);
+
  color:rgb(88,73,63);
display:block;
+
  display:block;
width:90px;
+
  width:90px;
min-height:2em;
+
  min-height:2em;
line-height:2em;
+
  line-height:2em;
text-align:center;
+
  text-align:center;
 
}
 
}
 
.top-nav ul li div a{
 
.top-nav ul li div a{
width:80px;
+
  width:80px;
  
 
}
 
}
 
/* menu when you hover */
 
/* menu when you hover */
 
.top-nav ul li .hover{
 
.top-nav ul li .hover{
background:rgb(118,191,141);
+
  background:rgb(118,191,141);
 
}
 
}
 
/* sub menu when you hover */
 
/* sub menu when you hover */
 
.top-nav ul li div{
 
.top-nav ul li div{
font-size:0.9em;
+
  font-size:0.9em;
width:80px;
+
  width:80px;
display:none;
+
  display:none;
position:relative;
+
  position:relative;
left:-80px;
+
  left:-80px;
top:-30px;
+
  top:-30px;
 
}
 
}
 
/* link style */
 
/* link style */
 
.top-nav ul li div a{
 
.top-nav ul li div a{
background:rgb(118,191,141);
+
  background:rgb(118,191,141);
color:rgb(88,73,63);
+
  color:rgb(88,73,63);
 
}
 
}
 
/* submenu link style */
 
/* submenu link style */
 
.top-nav ul li div a:hover{
 
.top-nav ul li div a:hover{
color:rgb(88,73,63);
+
  color:rgb(88,73,63);
background:rgb(118,191,141);
+
  background:rgb(118,191,141);
 
}
 
}
 
/* special  */
 
/* special  */
 
.top-nav #active{
 
.top-nav #active{
background:rgb(118,191,141);
+
  background:rgb(118,191,141);
 
}
 
}
  
Line 6,822: Line 6,822:
  
 
.logo{
 
.logo{
width:50px;
+
  width:50px;
position:absolute;
+
  position:absolute;
bottom:15px;
+
  bottom:15px;
left:250px;
+
  left:250px;
 
}
 
}
  
Line 6,833: Line 6,833:
 
/* the purple colomn at middle */
 
/* the purple colomn at middle */
 
.rolldown{
 
.rolldown{
display:inline-block;
+
  display:inline-block;
z-index:200;
+
  z-index:200;
position:absolute;
+
  position:absolute;
left:250px;
+
  left:250px;
width:950px;
+
  width:950px;
background:rgba(125, 63, 152, 0.4);
+
  background:rgba(125, 63, 152, 0.4);
min-height:1800px;
+
  min-height:1800px;
 
}
 
}
  
 
.header img{
 
.header img{
max-width: 950px;
+
  max-width: 950px;
 
     height: auto;
 
     height: auto;
 
}
 
}
Line 6,849: Line 6,849:
 
.responsive div.img {
 
.responsive div.img {
 
     margin: 5px;
 
     margin: 5px;
border:8px solid rgb(123,83,142);
+
  border:8px solid rgb(123,83,142);
 
     float: left;
 
     float: left;
 
     width: 210px;
 
     width: 210px;
Line 6,856: Line 6,856:
 
.responsive div.img:hover {
 
.responsive div.img:hover {
 
      
 
      
border:8px solid rgb(123,83,133);
+
  border:8px solid rgb(123,83,133);
 
}
 
}
  
Line 6,862: Line 6,862:
 
     width: 100%;
 
     width: 100%;
 
     height: auto;
 
     height: auto;
background-color:#fff;
+
  background-color:#fff;
 
}
 
}
  
 
.responsive div.desc {
 
.responsive div.desc {
min-height:220px;
+
  min-height:220px;
 
     padding: 15px;
 
     padding: 15px;
 
     text-align: center;
 
     text-align: center;
color:rgb(88,73,63);
+
  color:rgb(88,73,63);
background-color:rgb(215,196,221);
+
  background-color:rgb(215,196,221);
 
}
 
}
  
Line 6,881: Line 6,881:
  
  
<div class="container">  
+
  <div class="container">  
+
 
<div class="top-nav">
+
      <div class="top-nav">
<ul>
+
        <ul>
<li>
+
          <li>
 
                   <li>
 
                   <li>
<a href="#"><span>Team</span></a>                     
+
            <a href="#"><span>Team</span></a>                     
<div>
+
              <div>
<a href="https://2018.igem.org/Team:GreatBay_China/Team">Team</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Team">Team</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Collaborations">Collaboration</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Collaborations">Collaboration</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Attributions">Attributions</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Attributions">Attributions</a>  
</div>  
+
              </div>  
</li>
+
          </li>
<li>
+
          <li>
<a href="#"><span>Project</span></a>
+
            <a href="#"><span>Project</span></a>
<div>
+
              <div>
<a href="https://2018.igem.org/Team:GreatBay_China/Description">Description</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Description">Description</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Design">Design</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Design">Design</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Experiments">Experiments</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Experiments">Experiments</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Results" >Results</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Results" >Results</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Demonstrate">Demonstrate</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Demonstrate">Demonstrate</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Improve" >Improve</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Improve" >Improve</a>  
</div>
+
              </div>
</li>
+
          </li>
<li>
+
          <li>
<a href="https://2018.igem.org/Team:GreatBay_China/Parts"><span>Parts</span></a>  
+
            <a href="https://2018.igem.org/Team:GreatBay_China/Parts"><span>Parts</span></a>  
<div>
+
              <div>
<a href="https://2018.igem.org/Team:GreatBay_China/Basic_Part">Basic</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Basic_Part">Basic</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Composite_Part">Composite</a>   
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Composite_Part">Composite</a>   
<a href="https://2018.igem.org/Team:GreatBay_China/Part_Collection">Collection</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Part_Collection">Collection</a>  
</div>  
+
              </div>  
+
         
</li>
+
          </li>
<li>
+
          <li>
<a href="https://2018.igem.org/Team:GreatBay_China/Human_Practices"><span>HP</span></a>
+
            <a href="https://2018.igem.org/Team:GreatBay_China/Human_Practices"><span>HP</span></a>
<div>  
+
              <div>  
<a href="https://2018.igem.org/Team:GreatBay_China/Engagement">Public <br>Engagement</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Engagement">Public <br>Engagement</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Applied_Design">Product  Design</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Applied_Design">Product  Design</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/HP/Gold_Integrated">Integrated HP</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/HP/Gold_Integrated">Integrated HP</a>  
</div>
+
              </div>
</li>
+
          </li>
<li>
+
          <li>
<a href="#"><span>Lab</span></a>
+
            <a href="#"><span>Lab</span></a>
<div>
+
              <div>
<a href="https://2018.igem.org/Team:GreatBay_China/Notebook">Notebook</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Notebook">Notebook</a>  
<a href="https://2018.igem.org/Team:GreatBay_China/Interlab">Interlab</a>  
+
                <a href="https://2018.igem.org/Team:GreatBay_China/Interlab">Interlab</a>  
</div>
+
              </div>
</li>
+
          </li>
<li>
+
          <li>
<a href="https://2018.igem.org/Team:GreatBay_China/Judging"><span>Judging</span></a>
+
            <a href="https://2018.igem.org/Team:GreatBay_China/Judging"><span>Judging</span></a>
<div>
+
              <div>
</div>
+
              </div>
</li>
+
          </li>
<li>
+
          <li>
<a href="https://2018.igem.org/Team:GreatBay_China/Safety"><span>Safety</span></a>
+
            <a href="https://2018.igem.org/Team:GreatBay_China/Safety"><span>Safety</span></a>
<div>
+
              <div>
</div>
+
              </div>
</li>
+
          </li>
<li>
+
          <li>
<a href="https://2018.igem.org/Team:GreatBay_China/Model"><span>Model</span></a>
+
            <a href="https://2018.igem.org/Team:GreatBay_China/Model"><span>Model</span></a>
</li>
+
          </li>
<div>
+
              <div>
</div>
+
              </div>
<li id="active">
+
          <li id="active">
<a href="https://2018.igem.org/Team:GreatBay_China"><span>Home</span></a>
+
            <a href="https://2018.igem.org/Team:GreatBay_China"><span>Home</span></a>
</li>
+
          </li>
<li id="top">
+
          <li id="top">
<a href="#top" target="_self"><span><i><h6>↑Top</h6></i></span></a>
+
            <a href="#top" target="_self"><span><i><h6>↑Top</h6></i></span></a>
</li>
+
          </li>
</ul>
+
        </ul>  
+
       
</div>
+
    </div>
+
       
+
       
+
 
<div class="rolldown">
+
    <div class="rolldown">
<div class="container">
+
      <div class="container">
+
     
<div class="row">
+
      <div class="row">
<div class="header">
+
        <div class="header">
<img src="https://static.igem.org/mediawiki/2018/7/74/T--GreatBay_China--Top.png" />
+
          <img src="https://static.igem.org/mediawiki/2018/7/74/T--GreatBay_China--Top.png" />
</div>
+
        </div>
</div>
+
      </div>
<div class="row" style="margin:30px">
+
      <div class="row" style="margin:30px">
<span style="height:250px"></span>
+
        <span style="height:250px"></span>
<h1  style="color:white;font-size:25px;"  class="text-center" ></br>mCATNIP:</br>microbial Compartmentalization AssisTed </br>Nepetalactol Ingredient Production</br></h1>
+
        <h1  style="color:white;font-size:25px;"  class="text-center" ></br>mCATNIP:</br>microbial Compartmentalization AssisTed </br>Nepetalactol Ingredient Production</br></h1>
</br>
+
        </br>
</br>
+
        </br>
+
       
<p><video src="https://static.igem.org/mediawiki/2018/e/e4/T--GreatBay_China--video1.mov" type="video/mov" height="auto" width="850" controls>
+
        <p><video src="https://static.igem.org/mediawiki/2018/e/e4/T--GreatBay_China--video1.mov" type="video/mov" height="auto" width="850" controls>
</p>
+
        </p>
</div>
+
      </div>
<div class="row ">
+
      <div class="row ">
<span style="height:250px"></span>
+
        <span style="height:250px"></span>
<div class="jumbotron">
+
        <div class="jumbotron">
<h2>Abstract</h2>
+
          <h2>Abstract</h2>
<h4>
+
          <h4>
<button type="button" class="btn btn-primary" title="Timshel"   
+
              <button type="button" class="btn btn-primary" title="Timshel"   
data-container="body" data-toggle="popover" data-placement="top"  
+
      data-container="body" data-toggle="popover" data-placement="top"  
data-content="Meow!">
+
      data-content="Meow!">
Nepetalactone
+
    Nepetalactone
</button> is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between <i>E. coli</i> and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE) stabilized promoters to regulate the heterologous gene expression in <i>E. coli</i>. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education.
+
  </button> is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between <i>E. coli</i> and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE) stabilized promoters to regulate the heterologous gene expression in <i>E. coli</i>. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education.
</h4>
+
          </h4>  
</div>
+
        </div>
</div>
+
      </div>
  
 
<div class="row" style="padding-left:30px">
 
<div class="row" style="padding-left:30px">
Line 7,004: Line 7,004:
 
     </a>
 
     </a>
 
     <div class="desc">  Safety has been a crucial concern throughout our project. See our creative approaches to lab safety and biosecurity on Safety page.
 
     <div class="desc">  Safety has been a crucial concern throughout our project. See our creative approaches to lab safety and biosecurity on Safety page.
<br> </div>
+
        <br> </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
Line 7,035: Line 7,035:
 
     </a>
 
     </a>
 
     <div class="desc">  Apart from the lab, we reached out to the community in many ways. We have met lovely people and here is our stories.
 
     <div class="desc">  Apart from the lab, we reached out to the community in many ways. We have met lovely people and here is our stories.
<br> </div>
+
      <br> </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
Line 7,055: Line 7,055:
 
     </a>
 
     </a>
 
     <div class="desc">GreatBay_China 2018 consists of 13 students and 7 high schools, 1 instructor, 5 advisors and 2 PIs. Get to know about these lovely people! Also see attributions and collaborations here.
 
     <div class="desc">GreatBay_China 2018 consists of 13 students and 7 high schools, 1 instructor, 5 advisors and 2 PIs. Get to know about these lovely people! Also see attributions and collaborations here.
</div>
+
          </div>
 
   </div>
 
   </div>
 
</div>
 
</div>
Line 7,065: Line 7,065:
 
     </a>
 
     </a>
 
     <div class="desc">  This is a special page to help judges on evaluating the project. Let us show you why we deserve medals and awards.
 
     <div class="desc">  This is a special page to help judges on evaluating the project. Let us show you why we deserve medals and awards.
<br></div>
+
          <br></div>
 
   </div>
 
   </div>
 
</div>
 
</div>
 
</div>
 
</div>
  
</div>
+
      </div>
+
 
</div>
+
    </div>
 
</div>
 
</div>
+
 
+
   
  
  
Line 7,081: Line 7,081:
 
</body>
 
</body>
  
           
+
             
 
</html>
 
</html>

Revision as of 16:10, 4 October 2018

GBC-Home


mCATNIP:
microbial Compartmentalization AssisTed
Nepetalactol Ingredient Production



Abstract

is the active ingredient in catnip, a feline attractant, and a potential green pesticide. It has a common precursor, nepetalactol, with monoterpene indole alkaloids (MIAs) which is a group of plant-derived compounds of great therapeutic value, such as vincristine (an anti-cancer drug). We aim to synthesize nepetalactol through a mutualistic division of labour between E. coli and yeast. Besides, we design, characterize, and use a library of transcription activator-like effectors (TALE) stabilized promoters to regulate the heterologous gene expression in E. coli. Our applied design conceives the future application of nepetalactone on stray cat control, which we consider as an opportunity for public engagement and education.

Project
Metabolic engineering can help solve social issues! We envision to apply nepetalactone in stray cats rescue by employing VSD, making the use of synthetic biology readily comprehensible to the public.
Safety
Safety has been a crucial concern throughout our project. See our creative approaches to lab safety and biosecurity on Safety page.
Parts
We have fantastic parts and we want to share them with the entire iGEM community! Explore our basic parts, composite parts and a brand
Lab
See how we have been working in the lab since May! We managed to achieve high yield of geraniol and successful co-culture in the end.
HP
Apart from the lab, we reached out to the community in many ways. We have met lovely people and here is our stories.
Model
Modeling tools aid in the rational design and make the engineered system predictable. Visit this page to take a closer look.
Team
GreatBay_China 2018 consists of 13 students and 7 high schools, 1 instructor, 5 advisors and 2 PIs. Get to know about these lovely people! Also see attributions and collaborations here.
Judging
This is a special page to help judges on evaluating the project. Let us show you why we deserve medals and awards.