Difference between revisions of "Team:Jilin China/Part Collection"
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<p>In this collection, we provide a total of 48 different RNA thermosensors, which can regulate the expression of genes with the changed temperature. When the temperature is lower than the melting temperature of the stem-loop, the SD sequence is sequestered and cannot be bind by the ribosome. When the temperature elevate until the stem-loop structure is open, the SD sequence is exposed and the translation begins. The RNA thermosensors we designed has been characterized and mathematically fitted to prove that they have different melting temperature, expression intensity and sensitivity. Users can choos the appropriate RNA thermosensors according to their own needs.</p> | <p>In this collection, we provide a total of 48 different RNA thermosensors, which can regulate the expression of genes with the changed temperature. When the temperature is lower than the melting temperature of the stem-loop, the SD sequence is sequestered and cannot be bind by the ribosome. When the temperature elevate until the stem-loop structure is open, the SD sequence is exposed and the translation begins. The RNA thermosensors we designed has been characterized and mathematically fitted to prove that they have different melting temperature, expression intensity and sensitivity. Users can choos the appropriate RNA thermosensors according to their own needs.</p> | ||
<p>Heat-inducible RNA thermosensor collection contains these parts:</p> | <p>Heat-inducible RNA thermosensor collection contains these parts:</p> | ||
| − | + | <table width="200" border="1"> | |
| − | + | <tbody> | |
| + | <tr><td>Part Name</td><td>Part Number</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-1</td><td>BBa_K2541001</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-2</td><td>BBa_K2541002</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-3</td><td>BBa_K2541003</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-4</td><td>BBa_K2541004</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-5</td><td>BBa_K2541005</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-6</td><td>BBa_K2541006</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-7</td><td>BBa_K2541007</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-8</td><td>BBa_K2541008</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-9</td><td>BBa_K2541009</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-10</td><td>BBa_K2541010</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-11</td><td>BBa_K2541011</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-12</td><td>BBa_K2541012</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-13</td><td>BBa_K2541013</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-14</td><td>BBa_K2541014</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-15</td><td>BBa_K2541015</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-16</td><td>BBa_K2541016</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-17</td><td>BBa_K2541017</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-18</td><td>BBa_K2541018</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-19</td><td>BBa_K2541019</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-20</td><td>BBa_K2541020</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-21</td><td>BBa_K2541021</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-22</td><td>BBa_K2541022</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-23</td><td>BBa_K2541023</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-24</td><td>BBa_K2541024</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-25</td><td>BBa_K2541025</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-26</td><td>BBa_K2541026</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-27</td><td>BBa_K2541027</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-28</td><td>BBa_K2541028</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-29</td><td>BBa_K2541029</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-30</td><td>BBa_K2541030</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-31</td><td>BBa_K2541031</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-32</td><td>BBa_K2541032</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-33</td><td>BBa_K2541033</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-34</td><td>BBa_K2541034</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-35</td><td>BBa_K2541035</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-36</td><td>BBa_K2541036</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-37</td><td>BBa_K2541037</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-38</td><td>BBa_K2541038</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-39</td><td>BBa_K2541039</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-40</td><td>BBa_K2541040</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-41</td><td>BBa_K2541041</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-42</td><td>BBa_K2541042</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-43</td><td>BBa_K2541043</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-44</td><td>BBa_K2541044</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-45</td><td>BBa_K2541045</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-46</td><td>BBa_K2541046</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-47</td><td>BBa_K2541047</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-48</td><td>BBa_K2541048</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-49</td><td>BBa_K2541049</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-50</td><td>BBa_K2541050</td></tr> | ||
| + | <tr><td>Heat-inducible RNA thermosensor-51</td><td>BBa_K2541051</td></tr> | ||
| + | </tbody> | ||
<p>Heat-inducible RNA thermosensor collection can cover melting temperature from 33 to 55 degrees with different intensity and sensitivity.</p> | <p>Heat-inducible RNA thermosensor collection can cover melting temperature from 33 to 55 degrees with different intensity and sensitivity.</p> | ||
<p>For more detailed principles of Heat-inducible RNA thermosensors, you can read our design page.</p> | <p>For more detailed principles of Heat-inducible RNA thermosensors, you can read our design page.</p> | ||
Revision as of 09:20, 8 October 2018
PART
COLLECTION
Part Collection
-
Editing Alert!
This page is used by the judges to evaluate your team for the medal criterion or award listed below.
Part Collection
Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.
While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
Note
This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.
Best Basic Part Special Prize
To be eligible for this award, this part must adhere to Registry sample submission guidelines and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this special prize, make sure you add your part number to your judging form and delete the box at the top of this page.
Please note: Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. -
Abstract
This year, Jilin_China made a RNA thermosensor toolkit for users, include four different types, which are heat-inducible RNA thermosensors, heat-repressible RNA thermosensors, cold-inducible RNA thermosensors and cold-repressible RNA thermosensors. For each type of RNA thermosensors, we have added many different Biobrick parts to the parts registry. And we make each type of thermosensors into a part collection, allow users choose appropriate thermosensors from the collection.
Our part collection including heat-inducible RNA thermosensors collection, heat-repressible RNA thermosensors collection and cold-repressible RNA thermosensors collection.
In order to make it easier for users to select an appropriate RNA thermosensor, we also developed a search engine for our toolkit, you can click here to enter the search engine page.
The introduction of each type of part collection is as followed:
-
Heat-inducible RNA thermosensor collection
In this collection, we provide a total of 48 different RNA thermosensors, which can regulate the expression of genes with the changed temperature. When the temperature is lower than the melting temperature of the stem-loop, the SD sequence is sequestered and cannot be bind by the ribosome. When the temperature elevate until the stem-loop structure is open, the SD sequence is exposed and the translation begins. The RNA thermosensors we designed has been characterized and mathematically fitted to prove that they have different melting temperature, expression intensity and sensitivity. Users can choos the appropriate RNA thermosensors according to their own needs.
Heat-inducible RNA thermosensor collection contains these parts:
Part Name Part Number Heat-inducible RNA thermosensor-1 BBa_K2541001 Heat-inducible RNA thermosensor-2 BBa_K2541002 Heat-inducible RNA thermosensor-3 BBa_K2541003 Heat-inducible RNA thermosensor-4 BBa_K2541004 Heat-inducible RNA thermosensor-5 BBa_K2541005 Heat-inducible RNA thermosensor-6 BBa_K2541006 Heat-inducible RNA thermosensor-7 BBa_K2541007 Heat-inducible RNA thermosensor-8 BBa_K2541008 Heat-inducible RNA thermosensor-9 BBa_K2541009 Heat-inducible RNA thermosensor-10 BBa_K2541010 Heat-inducible RNA thermosensor-11 BBa_K2541011 Heat-inducible RNA thermosensor-12 BBa_K2541012 Heat-inducible RNA thermosensor-13 BBa_K2541013 Heat-inducible RNA thermosensor-14 BBa_K2541014 Heat-inducible RNA thermosensor-15 BBa_K2541015 Heat-inducible RNA thermosensor-16 BBa_K2541016 Heat-inducible RNA thermosensor-17 BBa_K2541017 Heat-inducible RNA thermosensor-18 BBa_K2541018 Heat-inducible RNA thermosensor-19 BBa_K2541019 Heat-inducible RNA thermosensor-20 BBa_K2541020 Heat-inducible RNA thermosensor-21 BBa_K2541021 Heat-inducible RNA thermosensor-22 BBa_K2541022 Heat-inducible RNA thermosensor-23 BBa_K2541023 Heat-inducible RNA thermosensor-24 BBa_K2541024 Heat-inducible RNA thermosensor-25 BBa_K2541025 Heat-inducible RNA thermosensor-26 BBa_K2541026 Heat-inducible RNA thermosensor-27 BBa_K2541027 Heat-inducible RNA thermosensor-28 BBa_K2541028 Heat-inducible RNA thermosensor-29 BBa_K2541029 Heat-inducible RNA thermosensor-30 BBa_K2541030 Heat-inducible RNA thermosensor-31 BBa_K2541031 Heat-inducible RNA thermosensor-32 BBa_K2541032 Heat-inducible RNA thermosensor-33 BBa_K2541033 Heat-inducible RNA thermosensor-34 BBa_K2541034 Heat-inducible RNA thermosensor-35 BBa_K2541035 Heat-inducible RNA thermosensor-36 BBa_K2541036 Heat-inducible RNA thermosensor-37 BBa_K2541037 Heat-inducible RNA thermosensor-38 BBa_K2541038 Heat-inducible RNA thermosensor-39 BBa_K2541039 Heat-inducible RNA thermosensor-40 BBa_K2541040 Heat-inducible RNA thermosensor-41 BBa_K2541041 Heat-inducible RNA thermosensor-42 BBa_K2541042 Heat-inducible RNA thermosensor-43 BBa_K2541043 Heat-inducible RNA thermosensor-44 BBa_K2541044 Heat-inducible RNA thermosensor-45 BBa_K2541045 Heat-inducible RNA thermosensor-46 BBa_K2541046 Heat-inducible RNA thermosensor-47 BBa_K2541047 Heat-inducible RNA thermosensor-48 BBa_K2541048 Heat-inducible RNA thermosensor-49 BBa_K2541049 Heat-inducible RNA thermosensor-50 BBa_K2541050 Heat-inducible RNA thermosensor-51 BBa_K2541051 Heat-inducible RNA thermosensor collection can cover melting temperature from 33 to 55 degrees with different intensity and sensitivity.
For more detailed principles of Heat-inducible RNA thermosensors, you can read our design page.
>Click here to read the design of the Heat-inducible RNA thermosensors!< Heat-repressible RNA thermosensor collection
In order to make our RNA thermosensor toolkit more suitable for users' needs, we updated the toolkit to version 2.0. We added the RNase E-based heat-repressible RNA thermosensors, including 23 different thermosensors. The stem-loop structure of the thermosensor contains the recognition and cleavage site of RNase E. At the elevated temperature, the stem-loop structure is opened, and the mRNA will be degraded, which lead to the failure of translation.
Heat-repressible RNA thermosensor collection contain these parts:
For more detailed principles of Heat-inducible RNA thermosensors, you can read our design page.
>Click here to read the design of the Heat-repressible RNA thermosensors!< Cold repressible RNA thermosensor collection
To expand the melting temprerature range of the thermosenor, we have made a second upgrade to the toolkit. In toolkit 3.0, we added cold-repressible and cold inducible RNA thermosenor, and made 10 cold-repressible RNA thermosensors into a part collection.
Cold-repressible RNA thermosensor is based in the RNase III. The stem-loop structure of the thermosensor mRNA contains the recognition and cleavage site of RNase III. When the temperature is lower than the melting temperature, the RNase III will degrade mRNA.
Cold-repressible RNA thermosensor collection contain these parts:
Part Name Part Number Cold-repressible RNA thermosensor-1 BBa_K2541201 Cold-repressible RNA thermosensor-2 BBa_K2541202 Cold-repressible RNA thermosensor-3 BBa_K2541203 Cold-repressible RNA thermosensor-4 BBa_K2541204 Cold-repressible RNA thermosensor-5 BBa_K2541205 Cold-repressible RNA thermosensor-6 BBa_K2541206 Cold-repressible RNA thermosensor-7 BBa_K2541207 Cold-repressible RNA thermosensor-8 BBa_K2541208 Cold-repressible RNA thermosensor-9 BBa_K2541209 Cold-repressible RNA thermosensor-10 BBa_K2541210 For more detailed principles of Heat-inducible RNA thermosensors, you can read our design page.
>Click here to read the design of the Cold-repressible RNA thermosensors!< Referrence