Difference between revisions of "Team:Pasteur Paris/Medals"
| Line 102: | Line 102: | ||
<tr> | <tr> | ||
<td><b>Validated Part / Validated Contribution</b></td> | <td><b>Validated Part / Validated Contribution</b></td> | ||
| − | <td>We created a new BioBrick part as expected : <a href="http://parts.igem.org/Part:BBa_K2616000" style="font-weight: bold ; color:black; text-decoration:none;" target="_blank">BBa_K2616000</a>. This part permits to express pro-NGF and secrete it in the extracellular medium using E. coli type I secretion system. Since the export peptide is not processed when passing through the secretion pore, we separated pro-NGF from this 60 aminoacid long sequence by a cleaving site for TEV protease. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. We succeeded in characterizing our pro-NGF by sequencing and mass spectrometry but could not purify it from the media. </td> | + | <td>We created a new BioBrick part as expected : <a href="http://parts.igem.org/Part:BBa_K2616000" style="font-weight: bold ; color:black; text-decoration:none;" target="_blank">BBa_K2616000</a>. This part permits to express pro-NGF and secrete it in the extracellular medium using <i>E. coli</i> type I secretion system. Since the export peptide is not processed when passing through the secretion pore, we separated pro-NGF from this 60 aminoacid long sequence by a cleaving site for TEV protease. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. We succeeded in characterizing our pro-NGF by sequencing and mass spectrometry but could not purify it from the media. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 17:51, 14 October 2018
Bronze medal criteria
| Explanation | Criteria achieved |
| Registration and Giant Jamboree Attendance | We registered for the iGEM Giant Jamboree and we can't wait to be there! |
| Competition Deliverables | We met the deadlines for every required deliverable. |
| Attributions | We completed the attributions page on our wiki. |
| Characterization / Contribution | We successfully completed the InterLab Measurement Study. |
Silver medal criteria
| Explanation | Criteria achieved |
| Validated Part / Validated Contribution | We created a new BioBrick part as expected : BBa_K2616000. This part permits to express pro-NGF and secrete it in the extracellular medium using E. coli type I secretion system. Since the export peptide is not processed when passing through the secretion pore, we separated pro-NGF from this 60 aminoacid long sequence by a cleaving site for TEV protease. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system. We succeeded in characterizing our pro-NGF by sequencing and mass spectrometry but could not purify it from the media. |
| Collaboration | We hosted the 4th Parisian Meet-Up where we organized a Tiny Jamboree in the morning in order to practice with all the French teams for the Giant. We also organized and attended round tables about bioethics with multiples professionals during the afternoon. We collaborated with other iGEM teams for the Interlab (Sorbonne U Paris), by sharing and testing protocols (WPI Worcester) as well as other non-scientific collaborations. You can read about them on this page . |
| Human Practices | We took into account the advices of many professionals in the conception and confinement of our interface NeuronArch. Our juridic team has worked with the Ethic Committeeof the Institut Pasteur on the ethic and safety issues surrounding the use of GMOs inside the human body and try to destigmatize the use of GMOs to the general public. Because we also thought about the consequence of a possible release of our GMOs in the environnement, we also integrated a thermosensitive Kill-Switch inside our bacteria. You can read about it on this page. |
Gold medal criteria
| Explanation | Criteria achieved |
| Integrated Human Practices | We thought with care of every aspect of our project by interviewing many experts of their fields (biofilms, infections, microfluidic and prosthesis) but also associations for the right of amputees as well as surgeons and took into account their advices in our final biological interface as well as our prototype. |
| Improve a Previous Part or Project | We improved the part BBa_K237002 from the iGEM team SDU Denmark 2009. We optimized the RIP sequence for our chassis E. coli BL21 (DE3) pLysS strain and added a secretion signal peptide to address the RIP to E. coli Type II Secretion System. We confirmed our part BBa_K2616001 by sequencing and are still waiting for the result from the mass spectrometry platform. |
| Model Your Project | We modeled how NGF is produced in our modified E. Coli , its diffusion in a medium environment and its consequences on neurons growth. It helped to have an insight on which concentration of NGF to use in our experiments and also helped in predicting the futur growth of nerves inside of prototype. The mechanical modeling presents tools to visualize the constraints applied on the humerus and femur bone and was used for choosing the best material to use for a prosthesis and the best configuration possible for a bone. |