Difference between revisions of "Team:Jilin China/Demonstrate"

Line 71: Line 71:
 
     <div>
 
     <div>
 
<h2>Demonstrate</h2>
 
<h2>Demonstrate</h2>
−
<h4 class="tables">·RNA-based thermosensor can achieve temperature sensing</h3>
+
<h4 class="tables"><b>·RNA-based thermosensors can achieve temperature sensing</b></h4>
 
<p>We designed four different types of RNA-based thermosensors, including heat-inducible RNA-based thermosensor, heat-repressible RNA-based thermosensor, cold-inducible RNA-based thermosensor and cold-repressible RNA-based thermosensor. We designed more than 200 different thermosensors, and constructed their measurement device. </p>
 
<p>We designed four different types of RNA-based thermosensors, including heat-inducible RNA-based thermosensor, heat-repressible RNA-based thermosensor, cold-inducible RNA-based thermosensor and cold-repressible RNA-based thermosensor. We designed more than 200 different thermosensors, and constructed their measurement device. </p>
 
<p>After the experiments, we got some inspired results. As <b>figure 1</b> shows, heat-inducible RNA-based thermosensors' activities increase at elevated temperature. Heat-repressible RNA-based thermosensors' activity decrease with increasement of temperature. Besides, cold-inducibe RNA-based thermosensors show lower sensing temperature range than heat-repressible RNA thermosensor, their intensity decrease sharply from 15 to 20℃. And cold-repressible RNA-based thermosensors' activity decrease with decreasing temperature even below 29℃.</p>
 
<p>After the experiments, we got some inspired results. As <b>figure 1</b> shows, heat-inducible RNA-based thermosensors' activities increase at elevated temperature. Heat-repressible RNA-based thermosensors' activity decrease with increasement of temperature. Besides, cold-inducibe RNA-based thermosensors show lower sensing temperature range than heat-repressible RNA thermosensor, their intensity decrease sharply from 15 to 20℃. And cold-repressible RNA-based thermosensors' activity decrease with decreasing temperature even below 29℃.</p>
 
<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/29/T--Jilin_China--demonstrate--hot.png" width="75%"/></div>
 
<div align="center"><img src="https://static.igem.org/mediawiki/2018/2/29/T--Jilin_China--demonstrate--hot.png" width="75%"/></div>
 
<center>Figure 1. Heat map of four different types of RNA-based thermosensors. Rows represent activity levels of different thermosensors. The activity levels are the mean of three replications. These values are normalized using the fluorescence/Abs600 of positive control. (A) includes 48 heat-inducible RNA-based thermosensors' activities at 29, 31, 35, 37, 39 and 42℃. (B) includes 23 heat-repressible RNA-based thermosensors' activities at 29, 37 and 42℃. (C) includes 10 cold-repressible RNA-based thermosensors' activities at 15, 25, 29, 35 and 37℃. (D) includes 8 cold-inducible RNA-based thermosensors' activities at 15, 20, 25℃.</center>
 
<center>Figure 1. Heat map of four different types of RNA-based thermosensors. Rows represent activity levels of different thermosensors. The activity levels are the mean of three replications. These values are normalized using the fluorescence/Abs600 of positive control. (A) includes 48 heat-inducible RNA-based thermosensors' activities at 29, 31, 35, 37, 39 and 42℃. (B) includes 23 heat-repressible RNA-based thermosensors' activities at 29, 37 and 42℃. (C) includes 10 cold-repressible RNA-based thermosensors' activities at 15, 25, 29, 35 and 37℃. (D) includes 8 cold-inducible RNA-based thermosensors' activities at 15, 20, 25℃.</center>
 +
 +
<h4 class="tables"><b>·Features of RNA-based thermosensors can be computed using fitted curve</b></h4>
 +
<p>In order to make users select a thermosensor conveniently by getting the melting temperature, intensity and sensitivity of the thermosensors, we fitted a curve to reflect the relationship between the change of temperature and the expression intensity of thermosensors (Figure 2).  </p>
 +
<center>Figure 2. </center>
 +
 
   
 
   
 
     </div>
 
     </div>

Revision as of 13:58, 15 October 2018

Project
Demonstration


Demonstrate

Demonstrate

  • Demonstrate

    ·RNA-based thermosensors can achieve temperature sensing

    We designed four different types of RNA-based thermosensors, including heat-inducible RNA-based thermosensor, heat-repressible RNA-based thermosensor, cold-inducible RNA-based thermosensor and cold-repressible RNA-based thermosensor. We designed more than 200 different thermosensors, and constructed their measurement device.

    After the experiments, we got some inspired results. As figure 1 shows, heat-inducible RNA-based thermosensors' activities increase at elevated temperature. Heat-repressible RNA-based thermosensors' activity decrease with increasement of temperature. Besides, cold-inducibe RNA-based thermosensors show lower sensing temperature range than heat-repressible RNA thermosensor, their intensity decrease sharply from 15 to 20℃. And cold-repressible RNA-based thermosensors' activity decrease with decreasing temperature even below 29℃.

    Figure 1. Heat map of four different types of RNA-based thermosensors. Rows represent activity levels of different thermosensors. The activity levels are the mean of three replications. These values are normalized using the fluorescence/Abs600 of positive control. (A) includes 48 heat-inducible RNA-based thermosensors' activities at 29, 31, 35, 37, 39 and 42℃. (B) includes 23 heat-repressible RNA-based thermosensors' activities at 29, 37 and 42℃. (C) includes 10 cold-repressible RNA-based thermosensors' activities at 15, 25, 29, 35 and 37℃. (D) includes 8 cold-inducible RNA-based thermosensors' activities at 15, 20, 25℃.

    ·Features of RNA-based thermosensors can be computed using fitted curve

    In order to make users select a thermosensor conveniently by getting the melting temperature, intensity and sensitivity of the thermosensors, we fitted a curve to reflect the relationship between the change of temperature and the expression intensity of thermosensors (Figure 2).

    Figure 2.