Materials

iGEM 2018 distribution kit

• List Item 1
• List Item 2

ddH₂O

Protocol

1. Add 10μL of ddH₂O to the desired well.
2. Pipette up and down 3-5 times.
3. Incubate at room temperature for 10 minutes.
4. Transform cells with 1μL of rehydrated DNA as per transformation protocol. Store the remaining amount at -20°C.

Materials

• Synthesized DNA from IDT or Genscript
• ddH₂O

Protocol

1. Centrifuge tube containing the synthesized DNA for 1 minute at 3000g.
2. Add 20μL ddH₂O.
3. Vortex for 1 minute.
4. Incubate at 50°C for 15 minutes.
5. Briefly centrifuge. Store at -20°C.

Materials

• Luria-Bertani broth with agar:
1. 10% (w/v) tryptone
2. 5% (w/v) NaCl
3. 10% (w/v) yeast extract
4. 15% (w/v) agar
• Appropriate antibiotic:

Protocols

1. Add 10μL of ddH₂O to the desired well.
2. Pipette up and down 3-5 times.
3. Incubate at room temperature for 10 minutes.
4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

Experimental Details

Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.

Materials

• iGEM 2017 distribution kit
• ddH₂O

Protocols

1. Add 10μL of ddH₂O to the desired well.
2. Pipette up and down 3-5 times.
3. Incubate at room temperature for 10 minutes.
4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

Experimental Details

Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.

Materials

• iGEM 2017 distribution kit
• ddH₂O

Protocols

1. Add 10μL of ddH₂O to the desired well.
2. Pipette up and down 3-5 times.
3. Incubate at room temperature for 10 minutes.
4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.

Experimental Details

Registry DNA was rehydrated for completion of the Interlab Study. Also, Part:BBa_K934001 (phaC1-A-B1) was rehydrated and transformed into our chassis so that PHB was produced and preliminary secretion assays could be performed before the Synthesis subgroup had completed their cloning.

Materials

• iGEM 2017 distribution kit
• ddH₂O

Protocols

1. Add 10μL of ddH₂O to the desired well.
2. Pipette up and down 3-5 times.
3. Incubate at room temperature for 10 minutes.
4. Transform cells with 1μL of rehydrated DNA. Store the remaining amount at -20°C.