Difference between revisions of "Team:Pasteur Paris/Demonstrate"
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| − | <h1>NERVE GROWTH FACTOR AND NEURON CULTURE</h1> | + | <h1>NERVE GROWTH FACTOR AND NEURON CULTURE</h1></div> |
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<p> We successfully cloned a part coding for secretion of NGF in pET43.1a and iGEM plasmid backbone pSB1C3, creating a new part <a href="http://parts.igem.org/Part:BBa_K2616000"style="font-weight: bold ; color:#85196a;"target="_blank"> Bba_K2616000 </a> and confirmed the production of proNGF by Western Blot and mass spectrometry. </p> | <p> We successfully cloned a part coding for secretion of NGF in pET43.1a and iGEM plasmid backbone pSB1C3, creating a new part <a href="http://parts.igem.org/Part:BBa_K2616000"style="font-weight: bold ; color:#85196a;"target="_blank"> Bba_K2616000 </a> and confirmed the production of proNGF by Western Blot and mass spectrometry. </p> | ||
<p> We grew neurons on our self-made microfluidic chips ans successfully observe axon growth in the presence of commercial NGF</p> | <p> We grew neurons on our self-made microfluidic chips ans successfully observe axon growth in the presence of commercial NGF</p> | ||
Revision as of 19:05, 16 October 2018
NERVE GROWTH FACTOR AND NEURON CULTURE
We successfully cloned a part coding for secretion of NGF in pET43.1a and iGEM plasmid backbone pSB1C3, creating a new part Bba_K2616000 and confirmed the production of proNGF by Western Blot and mass spectrometry.
We grew neurons on our self-made microfluidic chips ans successfully observe axon growth in the presence of commercial NGF
KILL SWITCH
Achievements:
- Successfully cloned a part coding for toxin/antitoxin (CcdB/CcdA) system in iGEM plasmid backbone, creating a new basic part Bba_K2616002
- Successfully sequenced BBa_K2616002 in pSB1C3 and sent to iGEM registry
- Successfully observe survival of our engineered bacteria at 25°C and 37°C and absence of growth at 18°C and 20°C, showing the efficiency of the kill switch
Next steps:
- Find a system that kills bacteria when released in the environment rather than just stopping their growth