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<div id="sec2"> | <div id="sec2"> | ||
<div id="page"> | <div id="page"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/f/f3/T--SMS_Shenzhen--expeTop.jpg" width="100%"> | ||
<h1>I Overview of Experiment Design Ideas</h1> | <h1>I Overview of Experiment Design Ideas</h1> | ||
− | <p style="font-size: 18px"> | + | <p style="font-size: 18px">There are three crucial aspects we can focus on to prove the plausibility of our design concept, including the preliminary characteristic determination of participant sugars, the enzyme activities of the two target enzymes in realistic conditions of oral cavities, and the optic observations about the enzyme effectiveness on real scale teeth.</p> |
− | < | + | |
+ | <b style="font-size: 20px">Abstract</b> | ||
+ | <p style="font-size: 18px">The goal of this experiment is to measure the physical properties, especially stability, of dextran, sucrose and glucose as the substrates and the products of the reaction. \nIn the experiment, a standard curve in relation between glucose concentration and absorbance will be determined under neutral pH condition in room temperature. Then, the stabilities of sugars will be measured by DNS chromogenic method, after being water bathing in different realistic temperatures and pH conditions simulating the oral cavity.</p> | ||
+ | |||
</div> | </div> | ||
</div> | </div> | ||
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<div id="page"> | <div id="page"> | ||
<h1>II Experiment Design</h1> | <h1>II Experiment Design</h1> | ||
− | <b style="font-size: 20px">General hypothesis</b> | + | <b style="font-size: 20px">1. General hypothesis</b> |
<p style="font-size: 18px">Here will be the general hypothesis</p> | <p style="font-size: 18px">Here will be the general hypothesis</p> | ||
− | < | + | <p style="font-size: 18px">Here will be the general hypothesis</p> |
− | <p style="font-size: 18px">Here will be the | + | <p style="font-size: 18px">Here will be the general hypothesis</p> |
+ | |||
+ | <b style="font-size: 20px">2. Preliminary Experiments: </b> | ||
+ | <p style="font-size: 18px">(1) Hypothesis: There is a linear relationship between Glucose concentration and the absorbance in 550nm.\n | ||
+ | (2)Aim: \na) Construct the standard curve between the concentration of glucose and its absorbance.\n b) Considering the effects of the external environment on Dextran and sucrose in the real reaction, we control the variables separately and design experiments on the pH and hydration of dextran under thermal conditions.\n(3) Design: </p> | ||
<b style="font-size: 20px">Design 2</b> | <b style="font-size: 20px">Design 2</b> |
Revision as of 13:19, 17 October 2018
I Overview of Experiment Design Ideas
There are three crucial aspects we can focus on to prove the plausibility of our design concept, including the preliminary characteristic determination of participant sugars, the enzyme activities of the two target enzymes in realistic conditions of oral cavities, and the optic observations about the enzyme effectiveness on real scale teeth.
AbstractThe goal of this experiment is to measure the physical properties, especially stability, of dextran, sucrose and glucose as the substrates and the products of the reaction. \nIn the experiment, a standard curve in relation between glucose concentration and absorbance will be determined under neutral pH condition in room temperature. Then, the stabilities of sugars will be measured by DNS chromogenic method, after being water bathing in different realistic temperatures and pH conditions simulating the oral cavity.
II Experiment Design
1. General hypothesisHere will be the general hypothesis
Here will be the general hypothesis
Here will be the general hypothesis
2. Preliminary Experiments:(1) Hypothesis: There is a linear relationship between Glucose concentration and the absorbance in 550nm.\n (2)Aim: \na) Construct the standard curve between the concentration of glucose and its absorbance.\n b) Considering the effects of the external environment on Dextran and sucrose in the real reaction, we control the variables separately and design experiments on the pH and hydration of dextran under thermal conditions.\n(3) Design:
Design 2Here will be the Design 2
Design 3Here will be the Design 3
III Modeling
General referencesHere will be the references
IV References
Design 1References for Design 1
Design 2References for Design 2
Design 3References for Design 3