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− | Despite the potential for establishing an ideal medicine, there are numerous challenges | + | Despite the potential for establishing an ideal medicine, there are numerous challenges facing development in this field. |
+ | In particular, limitations in selecting a specific target site for integration of genetic material, | ||
+ | avoidance of immune responses, methodology pertaining to gene delivery, | ||
+ | and maintenance of gene expression all pose a threat to the potential for gene therapy to become common practice.</p> | ||
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− | Snip, Equip, Flip is | + | Snip, Equip, Flip is a system that allows for the integration and maintenance of large-scale genetic constructs in eukaryotic systems</h1> |
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− | <p style="text-align: left">This year, the University of | + | <p style="text-align: left">This year, the University of Calgary aims to overcome the aforementioned obstacles related to large-scale genetic modification. |
+ | Our system allows for the creation of eukaryotic cell lines via stable integration and the expression of exogenous genes. | ||
+ | Developing a simple and reliable method that can be used by experienced and entrant researchers alike opens the door for further scientific discovery. | ||
+ | Ideally, the system introduced in this project will move beyond applications in research to form the foundation of a novel gene therapy. | ||
<br> | <br> | ||
<br> | <br> | ||
− | By complementing the specificity of | + | By complementing the specificity of CRISPR/Cas9 targeting with the significantly larger integration capabilities of FLP recombinase and beta resolvase, |
+ | gene integration into eukaryotic genomes becomes a simple process of Snip, Equip, Flip. | ||
+ | In addition, the expression of integrated genes can be protected and maintained over time by adding flanking chromatin modifying elements. | ||
</p> | </p> | ||
</div> | </div> | ||
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<div class="section"> | <div class="section"> | ||
− | <p style="text-align: left">Although most of our efforts centred around building the toolkit for gene integration into eukaryotic cells described above, we also built a multi-cloning site and introduced novel eukaryotic parts into the iGEM registry. Independent of the wet-lab work, the team created | + | <p style="text-align: left">Although most of our efforts centred around building the toolkit for gene integration into eukaryotic cells described above, |
+ | we also built a multi-cloning site and introduced novel eukaryotic parts into the iGEM registry. | ||
+ | Independent of the wet-lab work, the team created a droplet microfluidic device designed for a scalable gene transfer system and SARA, | ||
+ | a software tool built for iGEM participants to find past software projects. | ||
+ | The team also explored how our project could possibly affect current and future aspects of society through our human practices work.</p> | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 17:38, 17 October 2018
SNIP EQUIP FLIP
The ideal medicine is not a perfect treatment - it’s a cure.
However, major roadblocks still exist in implementation.
Advancements in the area of genetic modification have opened avenues towards numerous tracks of scientific development. In particular, the demand for permanent health solutions has sparked massive interest in gene therapy.
Despite the potential for establishing an ideal medicine, there are numerous challenges facing development in this field.
In particular, limitations in selecting a specific target site for integration of genetic material,
avoidance of immune responses, methodology pertaining to gene delivery,
and maintenance of gene expression all pose a threat to the potential for gene therapy to become common practice.
Our Answer
Snip, Equip, Flip is a system that allows for the integration and maintenance of large-scale genetic constructs in eukaryotic systems
This year, the University of Calgary aims to overcome the aforementioned obstacles related to large-scale genetic modification.
Our system allows for the creation of eukaryotic cell lines via stable integration and the expression of exogenous genes.
Developing a simple and reliable method that can be used by experienced and entrant researchers alike opens the door for further scientific discovery.
Ideally, the system introduced in this project will move beyond applications in research to form the foundation of a novel gene therapy.
By complementing the specificity of CRISPR/Cas9 targeting with the significantly larger integration capabilities of FLP recombinase and beta resolvase,
gene integration into eukaryotic genomes becomes a simple process of Snip, Equip, Flip.
In addition, the expression of integrated genes can be protected and maintained over time by adding flanking chromatin modifying elements.
Although most of our efforts centred around building the toolkit for gene integration into eukaryotic cells described above, we also built a multi-cloning site and introduced novel eukaryotic parts into the iGEM registry. Independent of the wet-lab work, the team created a droplet microfluidic device designed for a scalable gene transfer system and SARA, a software tool built for iGEM participants to find past software projects. The team also explored how our project could possibly affect current and future aspects of society through our human practices work.
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