Difference between revisions of "Team:Jilin China/Composite Part"
| Line 108: | Line 108: | ||
<p>The thermosensor sequence is constructed on the pSB1C3 vector by Golden Gate assembly. The measurement device is composed of Anderson promotor (BBa_J23104), thermosensor sequence, sfGFP_optimism (BBa_K2541400) and double terminator (BBa_B0010 and BBa_B0012). We select a constitutive Anderson promoter J23104 as an appropriate promoter by pre-experiment. The sfGFP_optimism has faster folding speed and higher fluorescence intensity. The double terminator can reduce leakage (Figure 1). We characterized RNA-based thermosensors in <i>E.coli</i> DH5a.</p> | <p>The thermosensor sequence is constructed on the pSB1C3 vector by Golden Gate assembly. The measurement device is composed of Anderson promotor (BBa_J23104), thermosensor sequence, sfGFP_optimism (BBa_K2541400) and double terminator (BBa_B0010 and BBa_B0012). We select a constitutive Anderson promoter J23104 as an appropriate promoter by pre-experiment. The sfGFP_optimism has faster folding speed and higher fluorescence intensity. The double terminator can reduce leakage (Figure 1). We characterized RNA-based thermosensors in <i>E.coli</i> DH5a.</p> | ||
<div align="center"><img src="https://static.igem.org/mediawiki/2018/5/54/T--Jilin_China--measure.svg" width="56%" /></div> | <div align="center"><img src="https://static.igem.org/mediawiki/2018/5/54/T--Jilin_China--measure.svg" width="56%" /></div> | ||
| − | <center>Figure | + | <center>Figure 1. Diagram of the measurement device.</center> |
</div> | </div> | ||
Revision as of 22:25, 17 October 2018
Composite Part
-
Abstract
This year, we added a total of 7 composite parts, including heat-inducible RNA-based thermosensor measurement device, heat-repressible RNA-based thermosensor measurement device, cold-inducible RNA-based thermosensor measurement device, cold-repressible RNA-based thermosensor measurement device, sfGFP_optimism measurement device, superfolder GFP measurement device and sfGFP(BbsI free)measurement device.
-
Thermosensor measurement device
Part Name Part Number Heat-inducible RNA-based thermosensor measurement device BBa_K2541405 Heat-repressible RNA-based thermosensor measurement device BBa_K2541406 Cold-repressible RNA-based thermosensor measurement device BBa_K2541407 Cold-inducible RNA-based thermosensor measurement device BBa_K2541408 The thermosensor sequence is constructed on the pSB1C3 vector by Golden Gate assembly. The measurement device is composed of Anderson promotor (BBa_J23104), thermosensor sequence, sfGFP_optimism (BBa_K2541400) and double terminator (BBa_B0010 and BBa_B0012). We select a constitutive Anderson promoter J23104 as an appropriate promoter by pre-experiment. The sfGFP_optimism has faster folding speed and higher fluorescence intensity. The double terminator can reduce leakage (Figure 1). We characterized RNA-based thermosensors in E.coli DH5a.
Figure 1. Diagram of the measurement device. -
sfGFP Measurement Device
In addition, we combined our cold-induced RNA-based thermosensors (cspA mRNA) with the promoter J23104 as a composite part for uploading, which is more conducive to other users directly, users can also only use a cold-induced RNA-based thermosensor according to their own needs.
Part Name Part Number sfGFP_optimism measurement device BBa_K2541402 superfolder GFP measurement device BBa_K2541403 sfGFP(BbsI free) measurement device BBa_K2541404 Introduction
They are three measurement device for sfGFP_optimism (BBa_K2541400), superfolder GFP (BBa_I746916) and sfGFP(BbsI free)(BBa_K2541401) fluorescence intensity, which is composed of Anderson promoter (BBa_J23104), RBS (BBa_B0034), sfGFP and double terminator (BBa_B0010 and BBa_B0012).