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+ | <meta charset="utf-8"> | ||
+ | <title>BJRS</title> | ||
+ | <link rel="stylesheet" href="https://cdn.bootcss.com/bootstrap/3.3.7/css/bootstrap.min.css"> | ||
+ | <script src="https://cdn.bootcss.com/jquery/2.1.1/jquery.min.js"></script> | ||
+ | <script src="https://cdn.bootcss.com/bootstrap/3.3.7/js/bootstrap.min.js"></script> | ||
+ | <style> | ||
+ | #contentSub, #footer-box, #catlinks, #search-controls, #p-logo, .printfooter, .firstHeading, .visualClear{display: none;} | ||
+ | #globalWrapper, #content { /*-- changes default wiki settings --*/ | ||
+ | width: 100%; | ||
+ | height: 100%; | ||
+ | border: 0px; | ||
+ | background-color:#00000000; | ||
+ | margin: 0px; | ||
+ | padding: 10px; | ||
+ | } | ||
− | <div class=" | + | html, body, .wrapper { /*-- changes default wiki settings --*/ |
− | < | + | width: 100%; |
− | < | + | height: 100%; |
− | Design | + | background-image:url(https://static.igem.org/mediawiki/2018/6/6c/T--BJRS_China--wikibg7v2.jpeg); |
− | </ | + | background-attachment: fixed; |
+ | background-size:1440px 1080px; | ||
+ | background-repeat:no-repeat; | ||
+ | } | ||
+ | #top-section { | ||
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+ | position: fixed; | ||
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+ | #menubar { | ||
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+ | font-family: Helvetica, Arial, Sans-serif; | ||
+ | font-size: 14px; | ||
+ | } | ||
+ | .top{ | ||
+ | height:141px; | ||
+ | width:100%; | ||
+ | position:fixed; | ||
+ | top:0px; | ||
+ | left:0px; | ||
+ | background:white; | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <nav class="navbar navbar-default" role="navigation"> | ||
+ | <div class="top container-fluid" style="position:fixed; z-index:999; top:0;"> | ||
+ | <div class="navbar-header"><a href="https://2018.igem.org/Team:BJRS_China" target="_top" class="navbar-brand"><img src="https://static.igem.org/mediawiki/2018/7/73/T--BJRS_China--logo.png" width="120px" height="120px" style="position:relative;top:0px;left:90%;" class="img-fluid rounded"><img src="https://static.igem.org/mediawiki/2018/b/b3/T--BJRS_China--logo2.jpeg"width="1200px" height="50px"style="position:relative;top:-114px;left:10px;"></a></div> | ||
+ | <div> | ||
+ | <ul class="nav navbar-nav"> | ||
+ | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> PROJECT <b class="caret"></b> </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Background">BACKGROUND</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Design">DESIGN</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Results">RESULTS</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Improve">IMPROVE</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/InterLab">INTERLAB</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Demonstrate">DEMONSTRATE</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Model">MODEL</a></li> | ||
+ | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> HUMAN PRACTICE <b class="caret"></b> </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Human_Practices">Human Practices</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Engagement">Engagement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ACHIEVEMENTS <b class="caret"></b> </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Parts">PARTS</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Judging_Criteria">JUDGING CRITERIA</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Safety">SAFETY</a></li> | ||
+ | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> ATTRIBUTION <b class="caret"></b> </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Attributions">ATTRIBUTION</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Collaborations">COLLABORATION</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"> <a href="#" class="dropdown-toggle" data-toggle="dropdown"> TEAM <b class="caret"></b> </a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Members">MEMBERS</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Notebooks">NOTEBOOKS</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Protocol">PROTOCOL</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BJRS_China/Acknowledgement">ACKNOWLEDGEMENT</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
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− | + | <h1 style = "text-align: center">Design</h1> | |
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− | <div | + | <h2>Overview</h2> |
− | < | + | <p><font size="4">  This year our project focused on enhancing the growth and production efficiency of the engineered bacteria during high-density fermentation. Previous research has found that expressing <i>Vitreoscilla</i> hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.<sup>[1]</sup> Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.<sup>[2]</sup> Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.</font></p> |
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Revision as of 18:04, 16 October 2018
Design
Overview
This year our project focused on enhancing the growth and production efficiency of the engineered bacteria during high-density fermentation. Previous research has found that expressing Vitreoscilla hemoglobin (VHb) inside the bacteria could leverage its property of combining and releasing oxygen to improve the efficiency of oxygen utilization and therefore enhance the efficiency of bacteria growth and production.[1] Follow-up studies have shown that expressing VHb in the periplasmic space could further improve that efficiency compared with intracellular expression.[2] Based on these, we proposed that expressing VHb outside the outer membrane using surface display system could further enhance the oxygen density surrounding the bacteria and improve oxygen utility in the system for further enhancement of the fermentation efficiency.