Difference between revisions of "Team:Jilin China/Attributions"

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       <h2>Temperature-induced Promoter Library Construction</h2>
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       <h2>Support</h2>
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       <p>Compared to widely used chemically induced promoters, physically induced promoters don’t require special inducer, reducing cost while avoiding super-sensitivity of inducer concentration. Besides, physically induced promoters have special characteristics and a vast application prospect, and among all kinds of  physical factors, temperature has become our main concern this year with its vast prospect in fermentation engineering, drug targeting and other fields. As a result, our project, with a view to construct and characterize synthetic regulated Temperature-Sensitive promoter libraries for 2018 iGEM competition.</p>
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      <h3>General Support</h3>
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       <p>According to current researches, there are three different types of biomolecules responsive to temperature: DNA, RNA and proteins. Actually, RNA is playing a dominant role in temperature sensing especially in bacteria. 5’UTR of mRNA forms secondary structure, in order to block or expose SD （Shine-Dalgarno sequence，binding to ribosomes in  prokaryote cells）sequence. Compared to DNA and proteins, it is easier to reconstruct temperature-sensing RNA.</p>
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       <p>Our primary objective is to construct a temperature-sensitive promoter library supplying the iGEM registry with temperature-sensitive promoter parts collection. However, natural temperature-sensitive promoters, which are in a small number lacking in standardization, are regulated by many trans-acting factors, we try to design and construct de novo synthetic temperature-sensitive promoters with current researches.
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      <h3>Project Support and Advice</h3>
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      <h3>Fundraising Help and Advice</h3>
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      <h3>Lab Support</h3>
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      <h3>Difficult Technique Support</h3>
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      <h3>Project Advisor Support</h3>
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      <h3>Wiki Support</h3>
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       <p>Thanks to ECharts and</p>
 
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Revision as of 05:07, 14 August 2018

ATTRIBUTIONS

Attributions

  • Editing Alert!

    This page is used by the judges to evaluate your team for the medal criterion or award

    To ensure what contents should be placed onto this page, check the guiding page!

    This page is your opportunity to explain what parts of your project you did and what was done by technicians, advisers, etc. This requirement is not about literature references - these can and should be displayed throughout your wiki.

    Bronze Medal Criterion #3

    All of the work done in your project must be attributed correctly on this page. You must clearly state the work that was done by the students on your team and note any work that was done by people outside of your team, including the host labs, advisors, instructors, and individuals not on the team roster.

    Please see the Medals requirements page for more details.

  • Team Accomplishment

    Clearly state what the team accomplished

  • Support

    General Support

    No text.

    Project Support and Advice

    No text.

    Fundraising Help and Advice

    No text.

    Lab Support

    No text.

    Difficult Technique Support

    No text.

    Project Advisor Support

    No text.

    Wiki Support

    Thanks to ECharts and

  • What are we doing?

    We construct our promoter library with the free combination of core promoter and 5＇UTR, then the expression efficiency is easily to be changed with the replacement of different core promoters and sequence changes of 5’UTR, realizing the bi-level regulation of both transcription and translation. Four synthetic standard regulated libraries have been constructed:

    • (1) Heat-induced RNA thermosensors library, directional reconstructions to secondary structures of RNA thermometers;
    • (2) Heat-repressible RNA thermosensors library, directional reconstructions to anti-RNaseE cleavage site;
    • (3) Cold-induced RNA thermosensors library, directional reconstructions to mRNA pseudoknot of CspA;
    • (4) Cold-repressible RNA thermosensors library, directional reconstructions to RNase III binding site.