Difference between revisions of "Team:Jilin China/InterLab"

Line 125: Line 125:
 
         <p>This is the transformation protocol.</p>
 
         <p>This is the transformation protocol.</p>
 
         <p>This is the interlab plate reader protocol.</p>
 
         <p>This is the interlab plate reader protocol.</p>
 +
    </div>
 +
    </li>
 +
 +
    <li class="" id="">
 +
    <div>
 +
      <h2>Raw Data</h2>
 +
<table width="200" border="1">
 +
<tbody>
 +
  <tr>
 +
    <td>&nbsp;</td>
 +
    <td>LUDOX CL-X</td>
 +
    <td>H2O</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 1</td>
 +
    <td>0.057</td>
 +
    <td>0.038</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 2</td>
 +
    <td>0.057</td>
 +
    <td>0.039</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 3</td>
 +
    <td>0.057</td>
 +
    <td>0.039</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 4</td>
 +
    <td>0.057</td>
 +
    <td>0.041</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Arith. Mean</td>
 +
    <td>0.057</td>
 +
    <td>0.039</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Corrected Abs600</td>
 +
    <td>0.018</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Reference OD600</td>
 +
    <td>0.063</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>OD600/Abs600</td>
 +
    <td>3.549</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</tbody>
 +
</table>
 +
<table width="200" border="1">
 +
<tbody>
 +
  <tr>
 +
    <td>Number of Particles</td>
 +
    <td>235294117.647</td>
 +
    <td>117647058.824</td>
 +
    <td>58823529.412</td>
 +
    <td>29411764.706</td>
 +
    <td>14705882.353</td>
 +
    <td>7352941.176</td>
 +
    <td>3676470.588</td>
 +
    <td>1838235.294</td>
 +
    <td>919117.647</td>
 +
    <td>459558.824</td>
 +
    <td>229779.412</td>
 +
    <td>0.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 1</td>
 +
    <td>0.703</td>
 +
    <td>0.369</td>
 +
    <td>0.199</td>
 +
    <td>0.141</td>
 +
    <td>0.1</td>
 +
    <td>0.066</td>
 +
    <td>0.051</td>
 +
    <td>0.044</td>
 +
    <td>0.043</td>
 +
    <td>0.041</td>
 +
    <td>0.041</td>
 +
    <td>0.039</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 2</td>
 +
    <td>0.644</td>
 +
    <td>0.375</td>
 +
    <td>0.219</td>
 +
    <td>0.12</td>
 +
    <td>0.084</td>
 +
    <td>0.064</td>
 +
    <td>0.053</td>
 +
    <td>0.044</td>
 +
    <td>0.041</td>
 +
    <td>0.038</td>
 +
    <td>0.04</td>
 +
    <td>0.039</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 3</td>
 +
    <td>0.638</td>
 +
    <td>0.359</td>
 +
    <td>0.209</td>
 +
    <td>0.122</td>
 +
    <td>0.08</td>
 +
    <td>0.059</td>
 +
    <td>0.048</td>
 +
    <td>0.044</td>
 +
    <td>0.042</td>
 +
    <td>0.041</td>
 +
    <td>0.043</td>
 +
    <td>0.04</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 4</td>
 +
    <td>0.651</td>
 +
    <td>0.365</td>
 +
    <td>0.204</td>
 +
    <td>0.123</td>
 +
    <td>0.08</td>
 +
    <td>0.058</td>
 +
    <td>0.047</td>
 +
    <td>0.044</td>
 +
    <td>0.042</td>
 +
    <td>0.042</td>
 +
    <td>0.04</td>
 +
    <td>0.039</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Arith. Mean</td>
 +
    <td>0.659</td>
 +
    <td>0.367</td>
 +
    <td>0.208</td>
 +
    <td>0.127</td>
 +
    <td>0.086</td>
 +
    <td>0.062</td>
 +
    <td>0.05</td>
 +
    <td>0.044</td>
 +
    <td>0.042</td>
 +
    <td>0.041</td>
 +
    <td>0.041</td>
 +
    <td>0.039</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Arith. Std.Dev.</td>
 +
    <td>0.03</td>
 +
    <td>0.007</td>
 +
    <td>0.009</td>
 +
    <td>0.01</td>
 +
    <td>0.01</td>
 +
    <td>0.004</td>
 +
    <td>0.003</td>
 +
    <td>0.0</td>
 +
    <td>0.001</td>
 +
    <td>0.002</td>
 +
    <td>0.001</td>
 +
    <td>0.001</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Arith. Net Mean</td>
 +
    <td>0.62</td>
 +
    <td>0.328</td>
 +
    <td>0.168</td>
 +
    <td>0.087</td>
 +
    <td>0.047</td>
 +
    <td>0.022</td>
 +
    <td>0.011</td>
 +
    <td>0.005</td>
 +
    <td>0.003</td>
 +
    <td>0.001</td>
 +
    <td>0.002</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</tbody>
 +
</table>
 +
<table width="200" border="1">
 +
<tbody>
 +
  <tr>
 +
    <td>Particles / OD</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Number of Particles</td>
 +
    <td>235294117.647</td>
 +
    <td>117647058.824</td>
 +
    <td>58823529.412</td>
 +
    <td>29411764.706</td>
 +
    <td>14705882.353</td>
 +
    <td>7352941.176</td>
 +
    <td>3676470.588</td>
 +
    <td>1838235.294</td>
 +
    <td>919117.647</td>
 +
    <td>459558.824</td>
 +
    <td>229779.412</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Mean particles / Abs600</td>
 +
    <td>379659729.967</td>
 +
    <td>358953650.11</td>
 +
    <td>349101064.758</td>
 +
    <td>337097589.752</td>
 +
    <td>314564328.405</td>
 +
    <td>326797385.621</td>
 +
    <td>350140056.022</td>
 +
    <td>386996904.025</td>
 +
    <td>334224598.93</td>
 +
    <td>367647058.824</td>
 +
    <td>131302521.008</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Mean of med-high levels:</td>
 +
    <td>&nbsp;</td>
 +
    <td>337302803.729</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</tbody>
 +
</table>
 +
<table width="200" border="1">
 +
<tbody>
 +
  <tr>
 +
    <td>Fluorescein uM</td>
 +
    <td>10.0</td>
 +
    <td>5.0</td>
 +
    <td>2.5</td>
 +
    <td>1.25</td>
 +
    <td>0.625</td>
 +
    <td>0.312</td>
 +
    <td>0.156</td>
 +
    <td>0.078</td>
 +
    <td>0.039</td>
 +
    <td>0.02</td>
 +
    <td>0.01</td>
 +
    <td>0.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 1</td>
 +
    <td>77669.0</td>
 +
    <td>38692.0</td>
 +
    <td>20601.0</td>
 +
    <td>10396.0</td>
 +
    <td>5058.0</td>
 +
    <td>1898.0</td>
 +
    <td>1532.0</td>
 +
    <td>760.0</td>
 +
    <td>382.0</td>
 +
    <td>192.0</td>
 +
    <td>100.0</td>
 +
    <td>10.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 2</td>
 +
    <td>80872.0</td>
 +
    <td>37847.0</td>
 +
    <td>20419.0</td>
 +
    <td>10215.0</td>
 +
    <td>5014.0</td>
 +
    <td>2484.0</td>
 +
    <td>1212.0</td>
 +
    <td>618.0</td>
 +
    <td>329.0</td>
 +
    <td>155.0</td>
 +
    <td>81.0</td>
 +
    <td>11.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 3</td>
 +
    <td>80348.0</td>
 +
    <td>39774.0</td>
 +
    <td>19819.0</td>
 +
    <td>10018.0</td>
 +
    <td>4892.0</td>
 +
    <td>2221.0</td>
 +
    <td>1059.0</td>
 +
    <td>522.0</td>
 +
    <td>256.0</td>
 +
    <td>127.0</td>
 +
    <td>65.0</td>
 +
    <td>11.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Replicate 4</td>
 +
    <td>78408.0</td>
 +
    <td>39810.0</td>
 +
    <td>19893.0</td>
 +
    <td>10190.0</td>
 +
    <td>4987.0</td>
 +
    <td>2525.0</td>
 +
    <td>1201.0</td>
 +
    <td>608.0</td>
 +
    <td>309.0</td>
 +
    <td>159.0</td>
 +
    <td>97.0</td>
 +
    <td>12.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Arith. Mean</td>
 +
    <td>79324.25</td>
 +
    <td>39030.75</td>
 +
    <td>20183.0</td>
 +
    <td>10204.75</td>
 +
    <td>4987.75</td>
 +
    <td>2282.0</td>
 +
    <td>1251.0</td>
 +
    <td>627.0</td>
 +
    <td>319.0</td>
 +
    <td>158.25</td>
 +
    <td>85.75</td>
 +
    <td>11.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Arith. Std.Dev.</td>
 +
    <td>1530.028</td>
 +
    <td>944.399</td>
 +
    <td>386.012</td>
 +
    <td>154.677</td>
 +
    <td>70.22</td>
 +
    <td>289.269</td>
 +
    <td>199.872</td>
 +
    <td>98.583</td>
 +
    <td>52.083</td>
 +
    <td>26.625</td>
 +
    <td>16.153</td>
 +
    <td>0.816</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Arith. Net Mean</td>
 +
    <td>79313.25</td>
 +
    <td>39019.75</td>
 +
    <td>20172.0</td>
 +
    <td>10193.75</td>
 +
    <td>4976.75</td>
 +
    <td>2271.0</td>
 +
    <td>1240.0</td>
 +
    <td>616.0</td>
 +
    <td>308.0</td>
 +
    <td>147.25</td>
 +
    <td>74.75</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</tbody>
 +
</table>
 +
<table width="200" border="1">
 +
<tbody>
 +
  <tr>
 +
    <td>Fluorescein/a.u.</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Fluorescein uM</td>
 +
    <td>10.0</td>
 +
    <td>5.0</td>
 +
    <td>2.5</td>
 +
    <td>1.25</td>
 +
    <td>0.625</td>
 +
    <td>0.312</td>
 +
    <td>0.156</td>
 +
    <td>0.078</td>
 +
    <td>0.039</td>
 +
    <td>0.02</td>
 +
    <td>0.01</td>
 +
  </tr>
 +
  <tr>
 +
    <td>uM Fluorescein/a.u.</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
    <td>0.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Mean uM fluorescein / a.u.:</td>
 +
    <td>&nbsp;</td>
 +
    <td>0.0</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>MEFL / a.u.:</td>
 +
    <td>&nbsp;</td>
 +
    <td>768289204.589</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</tbody>
 +
</table>
 +
<table width="200" border="1">
 +
<tbody>
 +
  <tr>
 +
    <td>Fluorescence Raw Readings:</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Hour 0:</td>
 +
    <td>Neg. Control</td>
 +
    <td>Pos. Control</td>
 +
    <td>Device 1</td>
 +
    <td>Device 2</td>
 +
    <td>Device 3</td>
 +
    <td>Device 4</td>
 +
    <td>Device 5</td>
 +
    <td>Device 6</td>
 +
    <td>LB + Chlor (blank)</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 1</td>
 +
    <td>148.0</td>
 +
    <td>293.0</td>
 +
    <td>262.0</td>
 +
    <td>323.0</td>
 +
    <td>149.0</td>
 +
    <td>621.0</td>
 +
    <td>315.0</td>
 +
    <td>199.0</td>
 +
    <td>148.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 2</td>
 +
    <td>149.0</td>
 +
    <td>293.0</td>
 +
    <td>261.0</td>
 +
    <td>318.0</td>
 +
    <td>146.0</td>
 +
    <td>615.0</td>
 +
    <td>307.0</td>
 +
    <td>195.0</td>
 +
    <td>146.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 3</td>
 +
    <td>150.0</td>
 +
    <td>283.0</td>
 +
    <td>258.0</td>
 +
    <td>315.0</td>
 +
    <td>150.0</td>
 +
    <td>611.0</td>
 +
    <td>305.0</td>
 +
    <td>224.0</td>
 +
    <td>145.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 4</td>
 +
    <td>152.0</td>
 +
    <td>290.0</td>
 +
    <td>254.0</td>
 +
    <td>316.0</td>
 +
    <td>149.0</td>
 +
    <td>619.0</td>
 +
    <td>335.0</td>
 +
    <td>223.0</td>
 +
    <td>140.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 1</td>
 +
    <td>153.0</td>
 +
    <td>296.0</td>
 +
    <td>271.0</td>
 +
    <td>298.0</td>
 +
    <td>154.0</td>
 +
    <td>594.0</td>
 +
    <td>310.0</td>
 +
    <td>206.0</td>
 +
    <td>153.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 2</td>
 +
    <td>155.0</td>
 +
    <td>293.0</td>
 +
    <td>259.0</td>
 +
    <td>310.0</td>
 +
    <td>151.0</td>
 +
    <td>580.0</td>
 +
    <td>301.0</td>
 +
    <td>199.0</td>
 +
    <td>147.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 3</td>
 +
    <td>155.0</td>
 +
    <td>290.0</td>
 +
    <td>258.0</td>
 +
    <td>315.0</td>
 +
    <td>148.0</td>
 +
    <td>589.0</td>
 +
    <td>298.0</td>
 +
    <td>208.0</td>
 +
    <td>146.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 4</td>
 +
    <td>154.0</td>
 +
    <td>290.0</td>
 +
    <td>258.0</td>
 +
    <td>310.0</td>
 +
    <td>154.0</td>
 +
    <td>615.0</td>
 +
    <td>313.0</td>
 +
    <td>202.0</td>
 +
    <td>145.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Hour 6:</td>
 +
    <td>Neg. Control</td>
 +
    <td>Pos. Control</td>
 +
    <td>Device 1</td>
 +
    <td>Device 2</td>
 +
    <td>Device 3</td>
 +
    <td>Device 4</td>
 +
    <td>Device 5</td>
 +
    <td>Device 6</td>
 +
    <td>LB + Chlor (blank)</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 1</td>
 +
    <td>174.0</td>
 +
    <td>1097.0</td>
 +
    <td>766.0</td>
 +
    <td>1409.0</td>
 +
    <td>192.0</td>
 +
    <td>3976.0</td>
 +
    <td>794.0</td>
 +
    <td>591.0</td>
 +
    <td>143.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 2</td>
 +
    <td>187.0</td>
 +
    <td>1161.0</td>
 +
    <td>801.0</td>
 +
    <td>1486.0</td>
 +
    <td>204.0</td>
 +
    <td>4135.0</td>
 +
    <td>835.0</td>
 +
    <td>619.0</td>
 +
    <td>144.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 3</td>
 +
    <td>188.0</td>
 +
    <td>1170.0</td>
 +
    <td>799.0</td>
 +
    <td>1548.0</td>
 +
    <td>206.0</td>
 +
    <td>4242.0</td>
 +
    <td>830.0</td>
 +
    <td>624.0</td>
 +
    <td>149.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 4</td>
 +
    <td>195.0</td>
 +
    <td>1196.0</td>
 +
    <td>850.0</td>
 +
    <td>1504.0</td>
 +
    <td>201.0</td>
 +
    <td>4279.0</td>
 +
    <td>836.0</td>
 +
    <td>624.0</td>
 +
    <td>143.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 1</td>
 +
    <td>179.0</td>
 +
    <td>1115.0</td>
 +
    <td>1732.0</td>
 +
    <td>2000.0</td>
 +
    <td>190.0</td>
 +
    <td>4127.0</td>
 +
    <td>793.0</td>
 +
    <td>603.0</td>
 +
    <td>147.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 2</td>
 +
    <td>187.0</td>
 +
    <td>1152.0</td>
 +
    <td>1675.0</td>
 +
    <td>2071.0</td>
 +
    <td>207.0</td>
 +
    <td>4478.0</td>
 +
    <td>821.0</td>
 +
    <td>616.0</td>
 +
    <td>149.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 3</td>
 +
    <td>190.0</td>
 +
    <td>1105.0</td>
 +
    <td>1734.0</td>
 +
    <td>2090.0</td>
 +
    <td>203.0</td>
 +
    <td>4370.0</td>
 +
    <td>823.0</td>
 +
    <td>617.0</td>
 +
    <td>151.0</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 4</td>
 +
    <td>185.0</td>
 +
    <td>1157.0</td>
 +
    <td>1705.0</td>
 +
    <td>2105.0</td>
 +
    <td>204.0</td>
 +
    <td>4386.0</td>
 +
    <td>838.0</td>
 +
    <td>624.0</td>
 +
    <td>143.0</td>
 +
  </tr>
 +
</tbody>
 +
</table>
 +
<table width="200" border="1">
 +
<tbody>
 +
  <tr>
 +
    <td>Abs600 Raw Readings:</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Hour 0:</td>
 +
    <td>Neg. Control</td>
 +
    <td>Pos. Control</td>
 +
    <td>Device 1</td>
 +
    <td>Device 2</td>
 +
    <td>Device 3</td>
 +
    <td>Device 4</td>
 +
    <td>Device 5</td>
 +
    <td>Device 6</td>
 +
    <td>LB + Chlor (blank)</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 1</td>
 +
    <td>0.072</td>
 +
    <td>0.068</td>
 +
    <td>0.059</td>
 +
    <td>0.071</td>
 +
    <td>0.067</td>
 +
    <td>0.06</td>
 +
    <td>0.056</td>
 +
    <td>0.063</td>
 +
    <td>0.045</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 2</td>
 +
    <td>0.07</td>
 +
    <td>0.069</td>
 +
    <td>0.055</td>
 +
    <td>0.07</td>
 +
    <td>0.067</td>
 +
    <td>0.062</td>
 +
    <td>0.059</td>
 +
    <td>0.067</td>
 +
    <td>0.044</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 3</td>
 +
    <td>0.071</td>
 +
    <td>0.068</td>
 +
    <td>0.057</td>
 +
    <td>0.07</td>
 +
    <td>0.066</td>
 +
    <td>0.059</td>
 +
    <td>0.056</td>
 +
    <td>0.074</td>
 +
    <td>0.047</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 4</td>
 +
    <td>0.071</td>
 +
    <td>0.071</td>
 +
    <td>0.057</td>
 +
    <td>0.07</td>
 +
    <td>0.068</td>
 +
    <td>0.06</td>
 +
    <td>0.059</td>
 +
    <td>0.075</td>
 +
    <td>0.048</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 1</td>
 +
    <td>0.069</td>
 +
    <td>0.068</td>
 +
    <td>0.057</td>
 +
    <td>0.066</td>
 +
    <td>0.067</td>
 +
    <td>0.062</td>
 +
    <td>0.056</td>
 +
    <td>0.065</td>
 +
    <td>0.047</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 2</td>
 +
    <td>0.07</td>
 +
    <td>0.069</td>
 +
    <td>0.057</td>
 +
    <td>0.067</td>
 +
    <td>0.066</td>
 +
    <td>0.061</td>
 +
    <td>0.056</td>
 +
    <td>0.063</td>
 +
    <td>0.05</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 3</td>
 +
    <td>0.072</td>
 +
    <td>0.068</td>
 +
    <td>0.055</td>
 +
    <td>0.065</td>
 +
    <td>0.064</td>
 +
    <td>0.061</td>
 +
    <td>0.057</td>
 +
    <td>0.065</td>
 +
    <td>0.043</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 4</td>
 +
    <td>0.071</td>
 +
    <td>0.071</td>
 +
    <td>0.058</td>
 +
    <td>0.068</td>
 +
    <td>0.064</td>
 +
    <td>0.065</td>
 +
    <td>0.057</td>
 +
    <td>0.063</td>
 +
    <td>0.045</td>
 +
  </tr>
 +
  <tr>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Hour 6:</td>
 +
    <td>Neg. Control</td>
 +
    <td>Pos. Control</td>
 +
    <td>Device 1</td>
 +
    <td>Device 2</td>
 +
    <td>Device 3</td>
 +
    <td>Device 4</td>
 +
    <td>Device 5</td>
 +
    <td>Device 6</td>
 +
    <td>LB + Chlor (blank)</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 1</td>
 +
    <td>0.329</td>
 +
    <td>0.288</td>
 +
    <td>0.126</td>
 +
    <td>0.291</td>
 +
    <td>0.301</td>
 +
    <td>0.251</td>
 +
    <td>0.239</td>
 +
    <td>0.267</td>
 +
    <td>0.045</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 2</td>
 +
    <td>0.332</td>
 +
    <td>0.293</td>
 +
    <td>0.125</td>
 +
    <td>0.298</td>
 +
    <td>0.313</td>
 +
    <td>0.254</td>
 +
    <td>0.25</td>
 +
    <td>0.28</td>
 +
    <td>0.044</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 3</td>
 +
    <td>0.331</td>
 +
    <td>0.292</td>
 +
    <td>0.125</td>
 +
    <td>0.302</td>
 +
    <td>0.31</td>
 +
    <td>0.256</td>
 +
    <td>0.244</td>
 +
    <td>0.276</td>
 +
    <td>0.047</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 1, Replicate 4</td>
 +
    <td>0.339</td>
 +
    <td>0.296</td>
 +
    <td>0.13</td>
 +
    <td>0.3</td>
 +
    <td>0.309</td>
 +
    <td>0.257</td>
 +
    <td>0.246</td>
 +
    <td>0.278</td>
 +
    <td>0.047</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 1</td>
 +
    <td>0.308</td>
 +
    <td>0.294</td>
 +
    <td>0.184</td>
 +
    <td>0.313</td>
 +
    <td>0.294</td>
 +
    <td>0.25</td>
 +
    <td>0.248</td>
 +
    <td>0.264</td>
 +
    <td>0.046</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 2</td>
 +
    <td>0.32</td>
 +
    <td>0.298</td>
 +
    <td>0.186</td>
 +
    <td>0.321</td>
 +
    <td>0.305</td>
 +
    <td>0.266</td>
 +
    <td>0.259</td>
 +
    <td>0.267</td>
 +
    <td>0.045</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 3</td>
 +
    <td>0.321</td>
 +
    <td>0.289</td>
 +
    <td>0.182</td>
 +
    <td>0.318</td>
 +
    <td>0.299</td>
 +
    <td>0.259</td>
 +
    <td>0.258</td>
 +
    <td>0.268</td>
 +
    <td>0.043</td>
 +
  </tr>
 +
  <tr>
 +
    <td>Colony 2, Replicate 4</td>
 +
    <td>0.314</td>
 +
    <td>0.301</td>
 +
    <td>0.186</td>
 +
    <td>0.323</td>
 +
    <td>0.304</td>
 +
    <td>0.262</td>
 +
    <td>0.26</td>
 +
    <td>0.27</td>
 +
    <td>0.045</td>
 +
  </tr>
 +
</tbody>
 +
</table>
 
     </div>
 
     </div>
 
     </li>
 
     </li>

Revision as of 13:05, 23 September 2018

INTERLAB


Introduction Second Subtopic

Interlab

  • Bronze Medal Criterion #4

    Standard Tracks: Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.

    For teams participating in the InterLab study, all work must be shown on this page.

  • Introduction

    Negative Control (BBa_R0040)

    Positive Control (BBa_I20270)

    Test Device 1 (BBa_J364000)

    Test Device 2 (BBa_J364001)

    Test Device 3 (BBa_J364002)

    Test Device 4 (BBa_J364007)

    Test Device 5 (BBa_J364008)

    Test Device 6 (BBa_J364009)

  • Device We Received

    Negative Control BBa_R0040
    Positive Control BBa_I20270
    Test Device 1 BBa_J364000
    Test Device 2 BBa_J364001
    Test Device 3 BBa_J364002
    Test Device 4 BBa_J364003
    Test Device 5 BBa_J364004
    Test Device 6 BBa_J364005

  • Protocol

    We strictly followed the protocol provided by iGEM. We measured the experiment of OD600 reference point and fitted the fluorescence standard curve first and then detected GFP expression in our E.coli with the plate reader Synergy HT from BioTek.

    This is the transformation protocol.

    This is the interlab plate reader protocol.

  • Raw Data

      LUDOX CL-X H2O
    Replicate 1 0.057 0.038
    Replicate 2 0.057 0.039
    Replicate 3 0.057 0.039
    Replicate 4 0.057 0.041
    Arith. Mean 0.057 0.039
    Corrected Abs600 0.018  
    Reference OD600 0.063  
    OD600/Abs600 3.549  
    Number of Particles 235294117.647 117647058.824 58823529.412 29411764.706 14705882.353 7352941.176 3676470.588 1838235.294 919117.647 459558.824 229779.412 0.0
    Replicate 1 0.703 0.369 0.199 0.141 0.1 0.066 0.051 0.044 0.043 0.041 0.041 0.039
    Replicate 2 0.644 0.375 0.219 0.12 0.084 0.064 0.053 0.044 0.041 0.038 0.04 0.039
    Replicate 3 0.638 0.359 0.209 0.122 0.08 0.059 0.048 0.044 0.042 0.041 0.043 0.04
    Replicate 4 0.651 0.365 0.204 0.123 0.08 0.058 0.047 0.044 0.042 0.042 0.04 0.039
    Arith. Mean 0.659 0.367 0.208 0.127 0.086 0.062 0.05 0.044 0.042 0.041 0.041 0.039
    Arith. Std.Dev. 0.03 0.007 0.009 0.01 0.01 0.004 0.003 0.0 0.001 0.002 0.001 0.001
    Arith. Net Mean 0.62 0.328 0.168 0.087 0.047 0.022 0.011 0.005 0.003 0.001 0.002  
    Particles / OD                      
    Number of Particles 235294117.647 117647058.824 58823529.412 29411764.706 14705882.353 7352941.176 3676470.588 1838235.294 919117.647 459558.824 229779.412
    Mean particles / Abs600 379659729.967 358953650.11 349101064.758 337097589.752 314564328.405 326797385.621 350140056.022 386996904.025 334224598.93 367647058.824 131302521.008
    Mean of med-high levels:   337302803.729                  
    Fluorescein uM 10.0 5.0 2.5 1.25 0.625 0.312 0.156 0.078 0.039 0.02 0.01 0.0
    Replicate 1 77669.0 38692.0 20601.0 10396.0 5058.0 1898.0 1532.0 760.0 382.0 192.0 100.0 10.0
    Replicate 2 80872.0 37847.0 20419.0 10215.0 5014.0 2484.0 1212.0 618.0 329.0 155.0 81.0 11.0
    Replicate 3 80348.0 39774.0 19819.0 10018.0 4892.0 2221.0 1059.0 522.0 256.0 127.0 65.0 11.0
    Replicate 4 78408.0 39810.0 19893.0 10190.0 4987.0 2525.0 1201.0 608.0 309.0 159.0 97.0 12.0
    Arith. Mean 79324.25 39030.75 20183.0 10204.75 4987.75 2282.0 1251.0 627.0 319.0 158.25 85.75 11.0
    Arith. Std.Dev. 1530.028 944.399 386.012 154.677 70.22 289.269 199.872 98.583 52.083 26.625 16.153 0.816
    Arith. Net Mean 79313.25 39019.75 20172.0 10193.75 4976.75 2271.0 1240.0 616.0 308.0 147.25 74.75  
    Fluorescein/a.u.                      
    Fluorescein uM 10.0 5.0 2.5 1.25 0.625 0.312 0.156 0.078 0.039 0.02 0.01
    uM Fluorescein/a.u. 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0
    Mean uM fluorescein / a.u.:   0.0                  
    MEFL / a.u.:   768289204.589                  
    Fluorescence Raw Readings:                  
    Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
    Colony 1, Replicate 1 148.0 293.0 262.0 323.0 149.0 621.0 315.0 199.0 148.0
    Colony 1, Replicate 2 149.0 293.0 261.0 318.0 146.0 615.0 307.0 195.0 146.0
    Colony 1, Replicate 3 150.0 283.0 258.0 315.0 150.0 611.0 305.0 224.0 145.0
    Colony 1, Replicate 4 152.0 290.0 254.0 316.0 149.0 619.0 335.0 223.0 140.0
    Colony 2, Replicate 1 153.0 296.0 271.0 298.0 154.0 594.0 310.0 206.0 153.0
    Colony 2, Replicate 2 155.0 293.0 259.0 310.0 151.0 580.0 301.0 199.0 147.0
    Colony 2, Replicate 3 155.0 290.0 258.0 315.0 148.0 589.0 298.0 208.0 146.0
    Colony 2, Replicate 4 154.0 290.0 258.0 310.0 154.0 615.0 313.0 202.0 145.0
                       
    Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
    Colony 1, Replicate 1 174.0 1097.0 766.0 1409.0 192.0 3976.0 794.0 591.0 143.0
    Colony 1, Replicate 2 187.0 1161.0 801.0 1486.0 204.0 4135.0 835.0 619.0 144.0
    Colony 1, Replicate 3 188.0 1170.0 799.0 1548.0 206.0 4242.0 830.0 624.0 149.0
    Colony 1, Replicate 4 195.0 1196.0 850.0 1504.0 201.0 4279.0 836.0 624.0 143.0
    Colony 2, Replicate 1 179.0 1115.0 1732.0 2000.0 190.0 4127.0 793.0 603.0 147.0
    Colony 2, Replicate 2 187.0 1152.0 1675.0 2071.0 207.0 4478.0 821.0 616.0 149.0
    Colony 2, Replicate 3 190.0 1105.0 1734.0 2090.0 203.0 4370.0 823.0 617.0 151.0
    Colony 2, Replicate 4 185.0 1157.0 1705.0 2105.0 204.0 4386.0 838.0 624.0 143.0
    Abs600 Raw Readings:                  
    Hour 0: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
    Colony 1, Replicate 1 0.072 0.068 0.059 0.071 0.067 0.06 0.056 0.063 0.045
    Colony 1, Replicate 2 0.07 0.069 0.055 0.07 0.067 0.062 0.059 0.067 0.044
    Colony 1, Replicate 3 0.071 0.068 0.057 0.07 0.066 0.059 0.056 0.074 0.047
    Colony 1, Replicate 4 0.071 0.071 0.057 0.07 0.068 0.06 0.059 0.075 0.048
    Colony 2, Replicate 1 0.069 0.068 0.057 0.066 0.067 0.062 0.056 0.065 0.047
    Colony 2, Replicate 2 0.07 0.069 0.057 0.067 0.066 0.061 0.056 0.063 0.05
    Colony 2, Replicate 3 0.072 0.068 0.055 0.065 0.064 0.061 0.057 0.065 0.043
    Colony 2, Replicate 4 0.071 0.071 0.058 0.068 0.064 0.065 0.057 0.063 0.045
                       
    Hour 6: Neg. Control Pos. Control Device 1 Device 2 Device 3 Device 4 Device 5 Device 6 LB + Chlor (blank)
    Colony 1, Replicate 1 0.329 0.288 0.126 0.291 0.301 0.251 0.239 0.267 0.045
    Colony 1, Replicate 2 0.332 0.293 0.125 0.298 0.313 0.254 0.25 0.28 0.044
    Colony 1, Replicate 3 0.331 0.292 0.125 0.302 0.31 0.256 0.244 0.276 0.047
    Colony 1, Replicate 4 0.339 0.296 0.13 0.3 0.309 0.257 0.246 0.278 0.047
    Colony 2, Replicate 1 0.308 0.294 0.184 0.313 0.294 0.25 0.248 0.264 0.046
    Colony 2, Replicate 2 0.32 0.298 0.186 0.321 0.305 0.266 0.259 0.267 0.045
    Colony 2, Replicate 3 0.321 0.289 0.182 0.318 0.299 0.259 0.258 0.268 0.043
    Colony 2, Replicate 4 0.314 0.301 0.186 0.323 0.304 0.262 0.26 0.27 0.045