Difference between revisions of "Team:Grenoble-Alpes/engineering"

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<li>pipette liquid in and out of Eppendorf tubes </p>
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/pipetting_module">lienpipette</a> liquid in and out of Eppendorf tubes </p>
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<li>heat and cool samples</p>
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<li>control the temperature of the samples</p>
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<li>extract DNA after the lysis step</p>
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<li>extract the DNA after the bacteriophages lysis step</p>
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<li>measure the fluorescence at the end of the process in order to know the results of our diagnostic</ul></p>
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<li>measure the fluorescence at the end of the process in order to get a diagnosis </ul></p>
 
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Revision as of 13:30, 9 October 2018

Template loop detected: Template:Grenoble-Alpes

ENGINEERING

In order to fully automate the whole biological process we need to be able to perform 4 main actions :

  • lienpipette liquid in and out of Eppendorf tubes

  • control the temperature of the samples

  • extract the DNA after the bacteriophages lysis step

  • measure the fluorescence at the end of the process in order to get a diagnosis

    To control those actions we use:
  • an Arduino microcontroller to handle the different electronic components
  • a raspberry pi (a very small and cheap computer) to display the results and interact with the user

The whole process takes place in a structure made of aluminum and Plexiglas. The samples, buffers and pipette tips are placed on a central rotating plate that moves thanks to a motor controlled via our Arduino.

This circular structure is very convenient as:

  • it optimizes the space used
  • it does not require to move the different modules, reducing the complexity of the system.

If you want to jump ahead to one of those modules, feel free to click on them !