Difference between revisions of "Team:Madrid-OLM/AptamerProtocols"
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<h3>SELEX</h3> | <h3>SELEX</h3> | ||
<h5>Prepare the library pool</h5> | <h5>Prepare the library pool</h5> | ||
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<ol> | <ol> | ||
| − | <li>Resuspend 2 nmol de la library pool on 200 µl of Binding Buffer (BB).</li> | + | <li> <p class="lead">Resuspend 2 nmol de la library pool on 200 µl of Binding Buffer (BB).</p></li> |
| − | <li>Denatured the library by heating it at 90ºC for 10 min and immediately cold it on ice for another 10 min.</li> | + | <li><p class="lead">Denatured the library by heating it at 90ºC for 10 min and immediately cold it on ice for another 10 min.</p></li> |
| − | <li>To get rid of the DNA that unespecifically binds to the system, apply the library through a nitrocellulose membrane and centrifuge 1 min at 8000 rpm. Quantify the DNA that does not bind unspeficically and note it as the initial DNA.</li> | + | <li><p class="lead">To get rid of the DNA that unespecifically binds to the system, apply the library through a nitrocellulose membrane and centrifuge 1 min at 8000 rpm. Quantify the DNA that does not bind unspeficically and note it as the initial DNA.</p></li> |
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</ol> | </ol> | ||
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Revision as of 12:26, 10 October 2018
Aptamer's Protocols
Texto de explicacion/ resumen de la pagina.