Line 137: | Line 137: | ||
<h3>2- PDMS casting</h3> | <h3>2- PDMS casting</h3> | ||
− | <div id=" | + | <div id="PDMSCas" class="tabs-container" data-content-align="center"> |
<ul class="tabs"> | <ul class="tabs"> | ||
<li class="active"> | <li class="active"> | ||
Line 173: | Line 173: | ||
</ul> | </ul> | ||
</div> | </div> | ||
+ | <br/><br/> | ||
+ | <a class="btn btn--primary-2 type--uppercase inner-link" href="#PDMSCas"> | ||
+ | <span class="btn__text"> | ||
+ | Back to PDMS casting Index | ||
+ | </span> | ||
+ | </a> | ||
− | <h3>3- | + | <h3>3- Plasma bonding</h3> |
− | <div id=" | + | <div id="PlamsB" class="tabs-container" data-content-align="center"> |
<ul class="tabs"> | <ul class="tabs"> | ||
<li class="active"> | <li class="active"> | ||
Line 207: | Line 213: | ||
</ul> | </ul> | ||
</div> | </div> | ||
+ | <br/><br/> | ||
+ | <a class="btn btn--primary-2 type--uppercase inner-link" href="#PlamsB"> | ||
+ | <span class="btn__text"> | ||
+ | Back to Plasma bonding Index | ||
+ | </span> | ||
+ | </a> | ||
+ | |||
+ | |||
+ | <h3>4- Closing the microfluidic circuit</h3> | ||
+ | |||
+ | <div id="Closingcir" class="tabs-container" data-content-align="center"> | ||
+ | <ul class="tabs"> | ||
+ | <li class="active"> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Choosing the pump, the tubing and the connectors</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Troubleshooting the leaks</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | |||
+ | <br/><br/> | ||
+ | <a class="btn btn--primary-2 type--uppercase inner-link" href="#Closingcir"> | ||
+ | <span class="btn__text"> | ||
+ | Back to Closing circuits Index | ||
+ | </span> | ||
+ | </a> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!--end of row--> | ||
+ | </div> | ||
+ | <!--end of container--> | ||
+ | </section> | ||
+ | |||
+ | |||
+ | <!--MADRID--> | ||
+ | <section id="Assem" class="text-center"> | ||
+ | <div class="container"> | ||
+ | <div class="row boxed boxed--border bg--secondary boxed--lg box-shadow"> | ||
+ | <div class="col-md-10 col-lg-10"> | ||
+ | <h2>Assemblies</h2> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <!--end of row--> | ||
+ | </div> | ||
+ | <!--end of container--> | ||
+ | </section> | ||
+ | </div> | ||
+ | <!--<div class="loader"></div>--> | ||
+ | <a class="back-to-top inner-link" href="#start" data-scroll-class="100vh:active"> | ||
+ | <i class="stack-interface stack-up-open-big"></i> | ||
+ | </a> | ||
+ | |||
+ | </body> | ||
+ | </html> | ||
+ | {{Madrid-OLM/footer}}{{Madrid-OLM/1}} | ||
+ | <html lang="en"> | ||
+ | <style> | ||
+ | .tittle-secc{ | ||
+ | padding-top: 12em !important; | ||
+ | } | ||
+ | .ourlist{ | ||
+ | font-size: 80% !important; | ||
+ | } | ||
+ | .nomargin{ | ||
+ | margin-bottom: 0.3em !important; | ||
+ | } | ||
+ | .tittlelist{ | ||
+ | margin-top: 15px !important; | ||
+ | margin-bottom: 10px !important; | ||
+ | } | ||
+ | .green{ | ||
+ | color: green; | ||
+ | } | ||
+ | .purple{ | ||
+ | color: purple; | ||
+ | } | ||
+ | .red{ | ||
+ | color: red; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <head> | ||
+ | <meta charset="utf-8"> | ||
+ | <title>Manufacturing Protocols</title> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <meta name="description" content="Protocols used un the descovery and characterization of the aptamers"> | ||
+ | </head> | ||
+ | <body class=" "> | ||
+ | <a id="start"></a> | ||
+ | <div class="main-container"> | ||
+ | <section class="page-navigator"> | ||
+ | <ul> | ||
+ | <li> | ||
+ | <a href="#home" class="inner-link" data-title="Home"></a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="#Chip" class="inner-link" data-title="Chip"></a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="#Assem" class="inner-link" data-title="Asemblies"></a> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </section> | ||
+ | |||
+ | <section class="tittle-secc text-center switchable feature-large"> | ||
+ | <div class="container"> | ||
+ | <div class="row justify-content-around"> | ||
+ | <div class="col-md-8 col-lg-8"> | ||
+ | <h1 id="Teamtittle">Manufacturing Protocols</h1> | ||
+ | <p class="lead"> | ||
+ | Texto de explicacion/ resumen de la pagina. | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!--end of row--> | ||
+ | </div> | ||
+ | <!--end of container--> | ||
+ | </section> | ||
+ | |||
+ | |||
+ | <!--Discovery protocols--> | ||
+ | <section id="Chip" class="text-center"> | ||
+ | <div class="container"> | ||
+ | <div class="row boxed boxed--border bg--secondary boxed--lg box-shadow"> | ||
+ | <div class="col-md-10 col-lg-10"> | ||
+ | <h2>Chip</h2> | ||
+ | |||
+ | <h3>1- Mold making</h3> | ||
+ | |||
+ | <div id="MoldM" class="tabs-container" data-content-align="center"> | ||
+ | <ul class="tabs"> | ||
+ | <li class="active"> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Laser engraver hacking</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | <h4>Laser engraver hacking</h4> | ||
+ | <p class="lead nomargin">Bill Of Materials: You could see a complete BoM here (upload the bill).</p> | ||
+ | <p class="lead nomargin">Amount of time: 3 hours.</p> | ||
+ | <p class="lead ">Total costs: 100 €.</p> | ||
+ | <p class="lead ">The laser usually come with a proprietary software that only allow you to engrave images. As the chips are going to be cuttered as a vector we’ll need to install a new laser firmware (into the laser) to be controlled with other software (into the PC connected to the laser).</p> | ||
+ | <ol class="ourlist"> | ||
+ | <li class="nomargin"> <p class="lead">Download the columns of the stl files from <a href="https://github.com/OpenLabMadrid/iGEM-Madrid-OLM/tree/master/Nitrocellulose%20columns">our github repository</a>.</p></li> | ||
+ | <li class="nomargin"><p class="lead">3D print the stl models in PETG. For more information about the reasons why we choose this material see the results page.</p></li> | ||
+ | |||
+ | |||
+ | |||
+ | </ol> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Software workflow</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Calibration</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Cutting</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <br/><br/> | ||
+ | <a class="btn btn--primary-2 type--uppercase inner-link" href="#MoldM"> | ||
+ | <span class="btn__text"> | ||
+ | Back to Mold making Index | ||
+ | </span> | ||
+ | </a> | ||
+ | |||
+ | |||
+ | |||
+ | <h3>2- PDMS casting</h3> | ||
+ | |||
+ | <div id="PDMSCas" class="tabs-container" data-content-align="center"> | ||
+ | <ul class="tabs"> | ||
+ | <li class="active"> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Preparing PDMS</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Making a vacuum pump and vacuum chamber</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Degassing</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Polymerization process</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <a class="btn btn--primary-2 type--uppercase inner-link" href="#PDMSCas"> | ||
+ | <span class="btn__text"> | ||
+ | Back to PDMS casting Index | ||
+ | </span> | ||
+ | </a> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | <h3>3- Plasma bonding</h3> | ||
+ | |||
+ | <div id="PlamsB" class="tabs-container" data-content-align="center"> | ||
+ | <ul class="tabs"> | ||
+ | <li class="active"> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Modding a microwave</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Calibrating the microwave</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <div class="tab__title"> | ||
+ | <span class="h5">Baking the PDMS</span> | ||
+ | </div> | ||
+ | <div class="tab__content"> | ||
+ | |||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | <a class="btn btn--primary-2 type--uppercase inner-link" href="#PlamsB"> | ||
+ | <span class="btn__text"> | ||
+ | Back to Plasma bonding Index | ||
+ | </span> | ||
+ | </a> | ||
<h3>4- Closing the microfluidic circuit</h3> | <h3>4- Closing the microfluidic circuit</h3> | ||
− | <div id=" | + | <div id="Closingcir" class="tabs-container" data-content-align="center"> |
<ul class="tabs"> | <ul class="tabs"> | ||
<li class="active"> | <li class="active"> | ||
Line 235: | Line 528: | ||
<br/><br/> | <br/><br/> | ||
− | <a class="btn btn--primary-2 type--uppercase inner-link" href="# | + | <a class="btn btn--primary-2 type--uppercase inner-link" href="#Closingcir"> |
<span class="btn__text"> | <span class="btn__text"> | ||
− | Back to | + | Back to Closing circuits Index |
</span> | </span> | ||
</a> | </a> |
Revision as of 13:19, 11 October 2018
Manufacturing Protocols
Texto de explicacion/ resumen de la pagina.
Chip
1- Mold making
-
Laser engraver hacking
Laser engraver hacking
Bill Of Materials: You could see a complete BoM here (upload the bill).
Amount of time: 3 hours.
Total costs: 100 €.
The laser usually come with a proprietary software that only allow you to engrave images. As the chips are going to be cuttered as a vector we’ll need to install a new laser firmware (into the laser) to be controlled with other software (into the PC connected to the laser).
-
Download the columns of the stl files from our github repository.
3D print the stl models in PETG. For more information about the reasons why we choose this material see the results page.
-
-
Software workflow
-
Calibration
-
Cutting
Back to Mold making Index
2- PDMS casting
-
Preparing PDMS
-
Making a vacuum pump and vacuum chamber
-
Degassing
-
Polymerization process
Back to PDMS casting Index
3- Plasma bonding
-
Modding a microwave
-
Calibrating the microwave
-
Baking the PDMS
Back to Plasma bonding Index
4- Closing the microfluidic circuit
-
Choosing the pump, the tubing and the connectors
-
Troubleshooting the leaks
Back to Closing circuits Index
Assemblies
Manufacturing Protocols
Texto de explicacion/ resumen de la pagina.
Chip
1- Mold making
-
Laser engraver hacking
Laser engraver hacking
Bill Of Materials: You could see a complete BoM here (upload the bill).
Amount of time: 3 hours.
Total costs: 100 €.
The laser usually come with a proprietary software that only allow you to engrave images. As the chips are going to be cuttered as a vector we’ll need to install a new laser firmware (into the laser) to be controlled with other software (into the PC connected to the laser).
-
Download the columns of the stl files from our github repository.
3D print the stl models in PETG. For more information about the reasons why we choose this material see the results page.
-
-
Software workflow
-
Calibration
-
Cutting
Back to Mold making Index
2- PDMS casting
-
Preparing PDMS
-
Making a vacuum pump and vacuum chamber
-
Degassing
-
Polymerization process
3- Plasma bonding
-
Modding a microwave
-
Calibrating the microwave
-
Baking the PDMS
4- Closing the microfluidic circuit
-
Choosing the pump, the tubing and the connectors
-
Troubleshooting the leaks
Back to Closing circuits Index