Difference between revisions of "Team:Grenoble-Alpes/temperature module"
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<li><a href="https://2018.igem.org/Team:Grenoble-Alpes/biology">BIOLOGY</a></li> | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/biology">BIOLOGY</a></li> | ||
| − | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/Hardware">ENGINEERING</a><ul><li> | + | <li><a href="https://2018.igem.org/Team:Grenoble-Alpes/Hardware" id="current-menu">ENGINEERING</a><ul><li> |
<a href="https://2018.igem.org/Team:Grenoble-Alpes/pipetting_module">PIPETTING MODULE</a> | <a href="https://2018.igem.org/Team:Grenoble-Alpes/pipetting_module">PIPETTING MODULE</a> | ||
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/fluorescence_module">FLUORESCENCE MODULE</a> | <a href="https://2018.igem.org/Team:Grenoble-Alpes/fluorescence_module">FLUORESCENCE MODULE</a> | ||
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| − | <a href="https://2018.igem.org/Team:Grenoble-Alpes/temperature_module" id="current- | + | <a href="https://2018.igem.org/Team:Grenoble-Alpes/temperature_module" id="current-el">TEMPERATURE MODULE</a> |
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<a href="https://2018.igem.org/Team:Grenoble-Alpes/purification_module">PURIFICATION MODULE</a> | <a href="https://2018.igem.org/Team:Grenoble-Alpes/purification_module">PURIFICATION MODULE</a> | ||
Revision as of 09:50, 12 October 2018
Template loop detected: Template:Grenoble-Alpes
TEMPERATURE MODULE
In the automated system, many steps require to heat or cool biological samples. Typically, the biological transformation is a good example: the samples containing the bacteria and the DNA need to be heated and cooled for precise durations at a constant temperature to realize a heat shock (a classical transformation goes from 0°C for 30 minutes to 42°C for 1 minute and back at 0°C for 2 minutes). Hence, this module has to be carefully considered because it is a key point to the success of any biological manipulation. In addition, because our system will be fully automated and nobody will be able to check the inside of the machine while it works, a reliable and robust module is required so that each time it is used, repeatable results are obtained.
In the following figure, the different biological steps are summarized and their required temperatures are given.
By design, each biological step will be contained in a module with a given temperature. This allows us to make temperature shocks by pipetting the liquid inside an Eppendorf tube from one place with a given temperature (0°C for instance) to another place in the machine with another temperature (42°C this time).
Design of the module
An Infra-Red temperature sensor placed on top of the Eppendorf tube can continuously sense the temperature of the solution from a distance (without disturbing or contaminating it) and send it to an Arduino.
A thermal conductor, placed around the tube and in contact with the heating/cooling device, eases and homogenizes the heat/cold transfer between the Eppendorf tube and the device producing heat/cold (to better homogenize the solution, an electronic pipette can also flush the solution).
The device in charge of heating/cooling the solution is placed under the Eppendorf tube. It is activated with the help of an Arduino and a MOS transistor. A goal temperature is programmed [1] in the Arduino and the heating/cooling device is switched on/off to be kept at this goal temperature (this way of programming the heating/cooling device powering is called an “all or nothing regulation”).