Difference between revisions of "Team:Jilin China/Result/Version 1"
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| + | <p>We constructed 150 heat-inducible RNA thermosensors using goldengate assembly described in the Construction page. And the measurement method was describled in Measurement page. </p> | ||
| + | <p>We measured the activities of these thermosensors at six different temperatures: 29, 31, 35, 37, 39 and 42℃.Upon screening, we selected 48 heat-inducible RNA thermosensors out of the 150 members. And figure 1 shows the 48 thermosensors' activity. The positive control we designed won't form stem-loop structure with SD sequence by software prediction, and the experimental charaterization is described in Measurement page. The normalized fluorescence are used to describe the change of activity compared with positive control. We find that all of these thermosensors' expression level increase at elevated temperature, and the extent of increase relative to temperature is different. </p> | ||
<img src="https://static.igem.org/mediawiki/2018/8/82/T--Jilin_China--result--RTbar.png" width="95%" length="95%"></img> | <img src="https://static.igem.org/mediawiki/2018/8/82/T--Jilin_China--result--RTbar.png" width="95%" length="95%"></img> | ||
| + | <p>To quantify different features of this library, we computed the fold-change of the response in the given temperature range. As the figure shows, from 29 to 37℃, the fold change ranges from x fold to x fold. And from 37 to 42℃, the fold change ranges from x fold to x fold. These fold-changes were higher than positive control. </p> | ||
</div> | </div> | ||
</li> | </li> | ||
Revision as of 14:16, 13 October 2018
VERSION 1.0
-
Results
We constructed 150 heat-inducible RNA thermosensors using goldengate assembly described in the Construction page. And the measurement method was describled in Measurement page.
We measured the activities of these thermosensors at six different temperatures: 29, 31, 35, 37, 39 and 42℃.Upon screening, we selected 48 heat-inducible RNA thermosensors out of the 150 members. And figure 1 shows the 48 thermosensors' activity. The positive control we designed won't form stem-loop structure with SD sequence by software prediction, and the experimental charaterization is described in Measurement page. The normalized fluorescence are used to describe the change of activity compared with positive control. We find that all of these thermosensors' expression level increase at elevated temperature, and the extent of increase relative to temperature is different.
To quantify different features of this library, we computed the fold-change of the response in the given temperature range. As the figure shows, from 29 to 37℃, the fold change ranges from x fold to x fold. And from 37 to 42℃, the fold change ranges from x fold to x fold. These fold-changes were higher than positive control.