Difference between revisions of "Team:Tianjin"

We successfully reconstructed the KaiABC circadian clock in Saccharomyces cerevisiae, predicting by modeling, regulating by expression control and characterizing its oscillation by yeast two-hybrid system. A bold attempt is made to explore how the oscillator can impact chromatin structure in yeast. The These results demonstrate that the heterologous circadian oscillator is transplantable for metabolic pathway study as well as wide-ranging applications.

Although the project barely involves genetic pollution or the use of toxic reagents, our instructor still attaches great importance to the lab safety during the experiment. Before entering the lab, every operator must accept comprehensive training and have a deep understanding of equipment and reagents. All wastes are divided and gathered into special bottles, later get handled by professionals in a unified manner.

We made a survey about the circadian clock to investigate the potential impact to society and conducted follow-up market research on possible application. Furthermore, we were devoted to spreading iGEM idea to every age group. We made a picture book and performed a TV show for children, invited teenagers and parents to our lab and displayed biology to hundreds of tourists on the Begonia Festival. Synthetic biology can be a popular science by our actual action.

Twelve years have passed since Professor Yingjin Yuan worked with MIT and successfully promoted the iGEM in Asia. iGEM has been all the rage in Tianjin University. This year, a group of dynastic teenagers have gone through a series of tough tests and eventually set up a united family. The wonderful summer will be kept in everyone’s mind. Thanks for everyone who once reached out to us.

We attach great importance to the construction and appliance of BioBrick Part. We submitted a variety of parts centering on core KaiABC oscillator, from single genes to cassettes with fusion proteins in yeast two-hybrid system. Clearly experimental characterization can be found in this page. Adapted cassettes about reporter genes and downstream application were also constructed. Besides, we redesign previous Nanoluc to serve our project better.

Based on the project, our model includes four parts. Taking the whole oscillation circuit into consideration, the Mars Model simulates the oscillation of KaiC phosphorylation. Yeast Two Hybrid Model improves the existing transcription and translation model, predicting the experiment data. The Evaluation Model effectively selects the suitable fluorescent protein among millions. Then a growth curve is built to illustrate and foresee the growth situation of yeasts we construct.