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| | <h2>Selex</h2> | | <h2>Selex</h2> |
| − | <p class="lead"><b>SELEX:</b> Systemic Evolution of Ligands via EXponential Selection is the process of identifying specific aptamers.</p> | + | <p class="lead"><b>SELEX:</b> Systematic Evolution of Ligands via Exponential Selection is the process of identifying specific aptamers.</p> |
| − | <p class="lead">To select a binding aptamer, you don't look for epitopes. this only simplifies the process as you don't design a determined structure but reduced little by little an already binding population. SELEX (Systemic Evolution of Ligands via EXponential Selection) is the process of identifying specific aptamers.</p> | + | <p class="lead">To select a binding aptamer, you don't look for epitopes. This only simplifies the process as you don't design a determined structure but reduced little by little an already binding population.</p> |
| − | | + | <p class="lead">The SELEX screening process starts with a random library of nucleotide oligomers of a fixed size with know sequences in each end (for further amplification by PCR). Then the library is incubated with the target molecule. A number of this random sequences will bound to the target and the unbound sequences will be discharged.</p> |
| | + | <p class="lead">The bound sequences are separated now from the target molecule (elution step) and amplified to create a new library.</p> |
| | + | <p class="lead">With every round, more and more oligonucleotides with low binding-affinity for our protein of interest will be discarded leaving only strong binding aptamers at the end:</p> |
| | + | <img alt="Image1" src="https://static.igem.org/mediawiki/2018/c/c3/T--Madrid-OLM--Aptamer--Discovery--Selexsquema.png" style="width:80%;"/> |
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