Difference between revisions of "Team:NEFU China/Basic Part"

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<title>Basic Parts</title>
 
<title>Basic Parts</title>
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  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
  <li class="mainlevel" id="mainlevel_03">
+
−
  <a href="https://2018.igem.org/Team:NEFU_China/Basic_Part"><img id="parts" src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--_PARTS.png">PARTS</a>
+
 +
<li class="mainlevel" id="mainlevel_03">
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Experiment"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENT</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
      <li class="mainlevel" id="mainlevel_04">
+
   
−
  <a href="https://2018.igem.org/Team:NEFU_China/Lock_Key"><img id="results" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">RESULTS</a>
+
−
  <ul id="sub_04">
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
+
−
  </ul>
+
−
  </li>
+
 
  <li class="mainlevel" id="mainlevel_05">
 
  <li class="mainlevel" id="mainlevel_05">
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
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  <ul id="sub_06">
 
  <ul id="sub_06">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">SOFTWARE1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">SOFTWARE2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 +
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
  </ul>
 
  </ul>
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  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
−
  </ul>
 
−
  </li>
 
−
  <li class="mainlevel" id="mainlevel_09">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Notebook"><img id="notebook" src="https://static.igem.org/mediawiki/2018/c/cb/T--NEFU_China--_NOTEBOOK.png">NOTEBOOK</a>
 
−
  <ul id="sub_09">
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">OVERVIRW</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
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</li>
 
</li>
 
</div>
 
</div>
−
<div id="lead">
+
<div class="layer-bottom">
−
    <div id="lead-content">
+
−
        <h1>Basic Parts</h1>
+
<canvas id="canvas" style="background:#000000"></canvas>
−
        <h2>Basic Parts's introduction.</h2>
+
 
−
    </div>
+
<script type="text/javascript">
 +
 +
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 3800;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
 +
 
 +
 
 +
var str1 = "ATCG";
 +
                        var str2 = "01";
 +
function draw(){
 +
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 +
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 +
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
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 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
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 +
                                for(var i=colunms/2;i<colunms;i++){
 +
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 +
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 +
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 +
context.fillText(str2[index],x,y);
 +
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 +
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 +
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 +
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 +
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 +
}
 +
drops[i]++;
 +
}
 +
};
 +
 
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
 +
 
 +
draw();
 +
setInterval(draw,60);
 +
};
 +
 
 +
</script>
 +
 +
           
 +
 
</div>
 
</div>
−
 
+
<header id="home-banner">
−
<div id="about" style="box-shadow: inset 0px 15px 15px -15px green;">
+
    <div class="row banner">
−
<div class="container">
+
        <div class="banner-text">
−
<div class="row">
+
            <h1 class="responsive-headline" style="color: yellow;font-size: 80px;">Basic part</h1>
−
                <div class="col-md-4">
+
           
−
                    <h2 class="heading">Basic Parts</h2>
+
        </div>
−
                </div>
+
−
                <div class="col-md-8">
+
−
                    <p>
+
−
                        IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
+
−
                    </p>
+
−
                </div>
+
−
        </div>
+
 
     </div>
 
     </div>
 +
</header>
 +
<div>
 +
<h1 style="font-size: 65px;color: orange!important;height: 84px;">Coding Book</h1>
 +
<table class="table">
 +
    <thead>
 +
<tr>
 +
  <th>Number</th>
 +
<th>Name</th>
 +
<th>Description</th>
 +
    </tr>
 +
    </thead>
 +
    <tbody>
 +
<tr>
 +
<td>[1]</td>
 +
<td><a href="#">pFUS1-EGFP-tCYC</a></td>
 +
 +
<td>Our team were aimed at adjusting the expression level of any existing promoter. Thus, we modified the Kozak sequence of the promoter in the plasmid BBa_K775004, a part from the University of the TU-Delft 2012 iGEM team. Our goal is to reduce the expression efficacy of this vector, and make it suitable to express a suicide gene for information destruction. To reduce the expression efficiency of this part in yeast, we modified the Kozak sequence by mutating the sequence “AAAAAA” to “GCCACC”. After that, our information destruction system was built and kill the information host yeast in a desired period of time. See more details in the basic part BBa_K2542014. We used the enhanced green fluorescent protein (EGFP) to test the effect of this modification. The generated plasmid allows a type yeast to sense the  factor secreted by -type yeast and express EGFP. Thus, when the promoter was activated by  factor secreted by -type yeast, the EGFP would be expressed. Thus, the EGFP expression would be the standard to tell the strength of our improved promoter.</td>
 +
</tr>
 +
<tr>
 +
<td>[2]</td>
 +
<td><a href="">pFig2c</a></td>
 +
 +
<td>Our team attempted to verify the strengthen of a promoter called Fig2c in a part of BBa_K1829002 from the iGEM 2015 UCSF team. For this purpose, we inserted the pFig2c promoter and EGFP coding sequence into the pesc-ura plasmid, and transferred it into a-type S. cerevisiaeThe expression of the Fig2c promoter was induced by adding a mating  factor. When the Fig2c promoter is induced, EGFP is expressed. In this way, we can detect the strengthen of the Fig2c promoter using EGFP as a reporter. </td>
 +
</tr>
 +
</tbody>
 +
</table>
 +
 
</div>
 
</div>
−
<hr style="text-align: center;margin-left: 150px;margin-right: 150px;">
+
 
−
<div class="humanpractice">
+
<div id="background-reference">
−
    <h2 class="heading">Basic Parts</h2>
+
<h1 style="font-size: 65px;color: yellow;height: 70px;">Reference</h1>
−
    <div id="title1" class="humanpractice-block">
+
<p> <a href="#"> [1] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>
−
        <h3>title：111111111111111</h3>
+
<br>
−
       
+
<a href="#"> [2] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>
−
        <p>
+
<br>
−
            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
+
<a href="#"> [3] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>
−
        </p>
+
<br>
−
    </div>
+
<a href="#"> [4] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>
−
    <div id="title2" class="humanpractice-block">
+
<br>
−
<h3>title：222222222222222</h3>
+
<a href="#"> [5] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>
−
+
<br>
−
<p>
+
<a href="#"> [6] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>s
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            IT WAS the best of times, it was the worst of times, it was theage of wisdom, it was the age of foolishness, it was the epoch ofbelief, it was the epoch of incredulity, it was the season of Light,it was the season of Darkness, it was the spring of hope, it was thewinter of despair, we had everything before us, we had nothingbefore us, we were all going direct to Heaven, we were all goingdirect the other way- in short, the period was so far like the presentperiod, that some of its noisiest authorities insisted on its beingreceived, for good or for evil, in the superlative degree ofcomparison only.
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Revision as of 19:26, 15 October 2018

Basic Parts

Coding Book

Number Name Description
[1] pFUS1-EGFP-tCYC Our team were aimed at adjusting the expression level of any existing promoter. Thus, we modified the Kozak sequence of the promoter in the plasmid BBa_K775004, a part from the University of the TU-Delft 2012 iGEM team. Our goal is to reduce the expression efficacy of this vector, and make it suitable to express a suicide gene for information destruction. To reduce the expression efficiency of this part in yeast, we modified the Kozak sequence by mutating the sequence “AAAAAA” to “GCCACC”. After that, our information destruction system was built and kill the information host yeast in a desired period of time. See more details in the basic part BBa_K2542014. We used the enhanced green fluorescent protein (EGFP) to test the effect of this modification. The generated plasmid allows a type yeast to sense the  factor secreted by -type yeast and express EGFP. Thus, when the promoter was activated by  factor secreted by -type yeast, the EGFP would be expressed. Thus, the EGFP expression would be the standard to tell the strength of our improved promoter.
[2] pFig2c Our team attempted to verify the strengthen of a promoter called Fig2c in a part of BBa_K1829002 from the iGEM 2015 UCSF team. For this purpose, we inserted the pFig2c promoter and EGFP coding sequence into the pesc-ura plasmid, and transferred it into a-type S. cerevisiaeThe expression of the Fig2c promoter was induced by adding a mating factor. When the Fig2c promoter is induced, EGFP is expressed. In this way, we can detect the strengthen of the Fig2c promoter using EGFP as a reporter.

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