Difference between revisions of "Team:NEFU China/Model2"

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<meta charset="utf-8">
 
<meta charset="utf-8">
 
<title>Model 2</title>
 
<title>Model 2</title>
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+
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-menu&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-members-foot&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-members-foot&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
−
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-software2&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
+
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-software1&action=raw&ctype=text/css"  rel="stylesheet" type="text/css">
  
 
<style>
 
<style>
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     margin-left: 0px;
 
     margin-left: 0px;
 
}
 
}
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.foot {
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#menu li ul li:hover ul{
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     font-size: 20px;
+
    background:rgba(0,0,0,0.75)!important;
 +
}
 +
li#mainlevel_01 a {
 +
     color: #FFE5B5!important;
 +
 +
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 +
li#mainlevel_01 a:hover {
 +
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 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
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                0px 0px 72px #FFE5B5,
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li#mainlevel_02 a {
 +
    color: #FFE5B5!important;
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li#mainlevel_02 a:hover {
 +
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 +
    text-shadow:0px 0px 8px #FFE5B5,
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 +
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li#mainlevel_03 a:hover {
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 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
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}
 +
li#mainlevel_05 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_05 a:hover {
 +
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 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
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}
 +
li#mainlevel_06 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_06 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
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 +
li#mainlevel_07 a {
 +
    color: #FFE5B5!important;
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 +
li#mainlevel_07 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_08 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_08 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
#menu li ul li ul li a:hover {
 +
    color: rgba(0,223,252,1);
 +
    border-top: dotted 1px rgba(255,255,255,0.91);
 +
    border-bottom: dotted 1px rgba(255,255,255,0.91);
 +
    background: rgba(0,223,252,.02);
 +
}
 +
#nav .mainlevel a {
 +
    color: black;
 +
    text-decoration:none;
 +
    line-height:32px;
 +
    display:block;
 +
    padding:0 5px;
 +
    font-size: 25px!important;
 +
font-family: 'Segoe UI', Roboto, 'Helvetica Neue', Arial, sans-serif, 'Apple Color Emoji', 'Segoe UI Emoji', 'Segoe UI Symbol', 'Noto Color Emoji' !important;
 +
}
 +
.layer-bottom {
 +
z-index: -2;
 +
position: absolute;
 +
margin-top: 0px!important;
 +
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 +
#foot-title h3{
 +
font-size: 18px!important;
 
}
 
}
 
</style>
 
</style>
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<body>
 
<body>
 
<!--menu-->
 
<!--menu-->
−
<div id="menu">
+
 
−
<li id="nav">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
+
<div id="menu" style="background-color:rgba(0,0,0,0.6)!important">
−
+
<li id="nav" style="left: 8%!important; width: 100%!important;">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
 +
<!--<a class="menu1" href="#" style="text-align: right;">☰</a>-->
 
<ul class="firstmenu" style="float: left">
 
<ul class="firstmenu" style="float: left">
 
 
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  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
  <li class="mainlevel" id="mainlevel_03">
+
−
  <a href="https://2018.igem.org/Team:NEFU_China/Basic_Part"><img id="parts" src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--_PARTS.png">PARTS</a>
+
 +
<li class="mainlevel" id="mainlevel_03">
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Experiment"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENT</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
      <li class="mainlevel" id="mainlevel_04">
+
   
−
  <a href="https://2018.igem.org/Team:NEFU_China/Lock_Key"><img id="results" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">RESULTS</a>
+
−
  <ul id="sub_04">
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
+
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
+
−
  </ul>
+
−
  </li>
+
 
  <li class="mainlevel" id="mainlevel_05">
 
  <li class="mainlevel" id="mainlevel_05">
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
Line 75: Line 173:
 
  <ul id="sub_06">
 
  <ul id="sub_06">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">SOFTWARE1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">SOFTWARE2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 84: Line 183:
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 +
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
  </ul>
 
  </ul>
Line 94: Line 194:
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
−
  </ul>
 
−
  </li>
 
−
  <li class="mainlevel" id="mainlevel_09">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Notebook"><img id="notebook" src="https://static.igem.org/mediawiki/2018/c/cb/T--NEFU_China--_NOTEBOOK.png">NOTEBOOK</a>
 
−
  <ul id="sub_09">
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">OVERVIRW</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
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</li>
 
</li>
 
</div>
 
</div>
−
<header id="home2">
+
<div class="layer-bottom">
 +
 +
<canvas id="canvas" style="background:#000000"></canvas>
 +
 
 +
<script type="text/javascript">
 +
 +
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 1200;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
 +
 
 +
 
 +
var str1 = "ATCG";
 +
                        var str2 = "01";
 +
function draw(){
 +
context.fillStyle = "rgba(0,0,0,0.2)";
 +
context.fillRect(0,0,W,H);
 +
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
for(var i=0;i<colunms/2;i++){
 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
                                for(var i=colunms/2;i<colunms;i++){
 +
var index = Math.floor(Math.random() * str2.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str2[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
};
 +
 
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
 +
 
 +
draw();
 +
setInterval(draw,60);
 +
};
 +
 
 +
</script>
 +
 +
           
 +
 +
</div>
 +
<header id="home-banner">
 
     <div class="row banner">
 
     <div class="row banner">
 
         <div class="banner-text">
 
         <div class="banner-text">
−
             <h1 class="responsive-headline">Model 2's Name</h1>
+
             <h1 class="responsive-headline" style="color: cyan!important;">Fighting Model</h1>
−
             <h3>Software's introduction.<br>
+
              
−
            </h3>
+
−
            <hr/>
+
 
         </div>
 
         </div>
 
     </div>
 
     </div>
 
</header>  
 
</header>  
−
<section id="resume" style="box-shadow: inset 0px 15px 15px -15px orange">
+
 
 +
<section id="resume" style="box-shadow: inset 0px 15px 15px -15px green">
 
<div class="row introduction" >
 
<div class="row introduction" >
 
<div class="three columns header-col">
 
<div class="three columns header-col">
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             <div class="row item">
 
             <div class="row item">
 
<div class="twelve columns">
 
<div class="twelve columns">
−
<h3>Model 2's title 1</h3>
 
−
<p class="info">Features:ssssssssssssssssssssssss</p>
 
 
<p>
 
<p>
−
It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
+
<br>
−
</p>
+
We determined the quantitative relationship of Fig2c promoter efficiency with spy yeast quantity, killer yeast amount, and the quantity of spy yeast secretion factor by model 1. Based on their relationships, we established the following models: First, we divided the experiment into two parts; the first part: type a yeast and type α yeast in the absence of secreted α factors, and competitive growth; the second part: under the condition of secreted α factor, the killer yeast is continuously being killed by the spy yeast. In order to measure the transcription efficiency of the Fig2c promoter, the activation efficiency of Fig2c promoter was determined when the Bax coding sequence was replaced by the coding sequence of enhancer green fluorescence protein (EGFP) in spy yeast. Secondly, in order to calculate the time duration for spy yeast to completely decease, 10 ml of the spy yeast and 50 l of the killer yeast (OD600nm is about 1.4) were mixed for cocultivation. Meanwhile, we used the cocultured spy yeast without integrated Bax gene and the killer yeast as a control groups. Finally, we could determine that the spy yeast could be completely eliminated after 14 h of the cocultivation. Based on these experimental data, a model was established.<br>
 +
According to our experiment, we build these two models:
 +
                    １） 竞争模型
 +
２）fighting模型：
 +
 +
 
 +
Suppose is correlation coeeficients, is quantity of yeast variance and the expression of Bax gene variance.<br>
 +
The result is as followed: it also verify our hypothesize is right.<br>
 +
                    </p>
 
</div>
 
</div>
 
             </div>  
 
             </div>  
−
            <div class="row item">
+
 
−
              <div class="twelve columns">
+
−
  <h3>Model 2's title 2</h3>
+
−
  <p class="info">Features:ssssssssssssssssssssssss</p>
+
−
  <p>
+
−
  It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
+
−
  </p>
+
−
                </div>
+
−
            </div>
+
−
    <div class="row item">
+
−
              <div class="twelve columns">
+
−
  <h3>Model 2's title 3</h3>
+
−
  <p class="info">Features:ssssssssssssssssssssssss</p>
+
−
  <p>
+
−
  It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
+
−
  </p>
+
−
                </div>
+
−
            </div>
+
 
         </div>  
 
         </div>  
 
       </div>
 
       </div>
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             <div class="row item">
 
             <div class="row item">
 
               <div class="twelve columns">
 
               <div class="twelve columns">
−
              <h3>Result 1</h3>
+
  <p><br>
−
                  <p class="info">Features：sssssssssssssss</p>
+
  The result is as followed: it also verify our hypothesize is right.
−
 
+
  </p>
−
                  <p>
+
</div>
−
                  It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
+
             </div>
−
                  </p>
+
−
              </div>
+
−
             </div>  
+
−
            <div class="row item">
+
−
              <div class="twelve columns">
+
−
                  <h3>Result 2</h3>
+
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                  <p class="info">Features：sssssssssssssss</p>
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                  It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
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                  <h3>Result 3</h3>
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                  <p class="info">Features：sssssssssssssss</p>
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                  It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
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            <h1><span>Others</span></h1>
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              <h3>Parameters</h3>
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                  It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
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                  <h3>Download</h3>
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                  It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way.
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</section>
 
</section>
 
<div class="foot">
 
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Revision as of 07:44, 16 October 2018

Model 2

intorduction


We determined the quantitative relationship of Fig2c promoter efficiency with spy yeast quantity, killer yeast amount, and the quantity of spy yeast secretion factor by model 1. Based on their relationships, we established the following models: First, we divided the experiment into two parts; the first part: type a yeast and type α yeast in the absence of secreted α factors, and competitive growth; the second part: under the condition of secreted α factor, the killer yeast is continuously being killed by the spy yeast. In order to measure the transcription efficiency of the Fig2c promoter, the activation efficiency of Fig2c promoter was determined when the Bax coding sequence was replaced by the coding sequence of enhancer green fluorescence protein (EGFP) in spy yeast. Secondly, in order to calculate the time duration for spy yeast to completely decease, 10 ml of the spy yeast and 50 l of the killer yeast (OD600nm is about 1.4) were mixed for cocultivation. Meanwhile, we used the cocultured spy yeast without integrated Bax gene and the killer yeast as a control groups. Finally, we could determine that the spy yeast could be completely eliminated after 14 h of the cocultivation. Based on these experimental data, a model was established.
According to our experiment, we build these two models: １） 竞争模型 ２）fighting模型： Suppose is correlation coeeficients, is quantity of yeast variance and the expression of Bax gene variance.
The result is as followed: it also verify our hypothesize is right.

RESULTS


The result is as followed: it also verify our hypothesize is right.

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