Difference between revisions of "Team:Rheda Bielefeld/OverviewExperiment"

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to prove the occurence of pectin; we were able to crack pollen and to clone Escherichia coli.
 
to prove the occurence of pectin; we were able to crack pollen and to clone Escherichia coli.
 
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After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>.  
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After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>. Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.
 
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Revision as of 12:40, 16 October 2018

Home
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Description Notebook
Overview Methods Pollen PCR Assays Cloning Strategy
Human Practices Marta Event
Safety Form Biosafety
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Overview Experiments

For our project, we needed to do a lot of experiments. At first, it was a bit difficult to find some well working methods, but after a while of researching we were finally able to get very good results.

We did two assays [1] [2] to prove the occurence of pectin; we were able to crack pollen and to clone Escherichia coli.

After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR [1] [3] . Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.

Pictures

Text