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After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>. Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before. | After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow;"> [1] </a> <a href="https://2018.igem.org/Team:Rheda_Bielefeld/PCR" style="color:yellow;"> [3] </a>. Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before. | ||
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− | Our team also dealed with searching for DNA sequences saved in the database of <a href="https://www.ncbi.nlm.nih.gov/genbank/"> NCBI </a>. How to use the program MEGA7 and the database is also mentioned in the Overview. | + | Our team also dealed with searching for DNA sequences saved in the database of <a href="https://www.ncbi.nlm.nih.gov/genbank/"> NCBI </a>.How to use the program MEGA7 and the database is also mentioned in the Overview. |
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Revision as of 14:10, 16 October 2018
Overview Experiments
We did two assays [1] [2] to prove the occurence of pectin; we were able to crack pollen and to clone Escherichia coli.
After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR [1] [3] . Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.
Our team also dealed with searching for DNA sequences saved in the database of NCBI .How to use the program MEGA7 and the database is also mentioned in the Overview.
Pictures
Working at the assays