Difference between revisions of "Team:Pasteur Paris/Demonstrate"
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<p> We successfully cloned a part coding for secretion of NGF in pET43.1a and iGEM plasmid backbone pSB1C3, <b>creating a new part</b> <a href="http://parts.igem.org/Part:BBa_K2616000"style="font-weight: bold ; color:#85196a;"target="_blank"> Bba_K2616000 </a> and <b>confirmed the production of proNGF</b> by Western Blot and mass spectrometry. </p> | <p> We successfully cloned a part coding for secretion of NGF in pET43.1a and iGEM plasmid backbone pSB1C3, <b>creating a new part</b> <a href="http://parts.igem.org/Part:BBa_K2616000"style="font-weight: bold ; color:#85196a;"target="_blank"> Bba_K2616000 </a> and <b>confirmed the production of proNGF</b> by Western Blot and mass spectrometry. </p> | ||
<p> We designed self-made <b>microfluidic device</b> in order to implement our final proof of concept. </p> | <p> We designed self-made <b>microfluidic device</b> in order to implement our final proof of concept. </p> | ||
| − | <p> We grew neurons on our self-made microfluidic chips and successfully <b> | + | <p> We grew neurons on our self-made microfluidic chips and successfully <b>observed axon growth</b> in the presence of commercial NGF.</p> |
</div> | </div> | ||
Revision as of 22:25, 16 October 2018
NERVE GROWTH FACTOR AND NEURON CULTURE
We successfully cloned a part coding for secretion of NGF in pET43.1a and iGEM plasmid backbone pSB1C3, creating a new part Bba_K2616000 and confirmed the production of proNGF by Western Blot and mass spectrometry.
We designed self-made microfluidic device in order to implement our final proof of concept.
We grew neurons on our self-made microfluidic chips and successfully observed axon growth in the presence of commercial NGF.
KILL SWITCH
We successfully cloned a part coding for toxin/antitoxin (CcdB/CcdA) system in iGEM plasmid backbone, creating a new part Bba_K2616002
We observed survival and normal growth of our engineered bacteria at 25°C and 37°C and absence of growth at 18°C and 20°C, showing the efficiency of the kill switch if our bacteria are released in the environment.