Difference between revisions of "Team:NEFU China/Splicing"

Line 12: Line 12:
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-background-foot&action=raw&ctype=text/css" rel="stylesheet" type="text/css">
 
<link href="https://2018.igem.org/wiki/index.php?title=Template:NEFU_China/CSS-background-foot&action=raw&ctype=text/css" rel="stylesheet" type="text/css">
 
<style>
 
<style>
 +
#table1,#table2,#table3,#table4,#table5,#table6,#table7 {background-color: transparent!important;}
  
 +
#table1,#table2,#table3,#table4,#table5,#table6,#table7 {background-color: transparent!important;}
 +
 +
#table1 tr {
 +
    background-color: transparent!important;
 +
}
 +
 +
#table1 td {
 +
    vertical-align: top;
 +
}
 +
#table2 td {
 +
    vertical-align: top;
 +
}
 +
#table3 td {
 +
    vertical-align: top;
 +
}
 +
#table4 td {
 +
    vertical-align: top;
 +
}
 +
#table5 td {
 +
    vertical-align: top;
 +
}
 +
#table6 td {
 +
    vertical-align: top;
 +
}
 +
#table7 td {
 +
    vertical-align: top;
 +
}
 
#top_title, #sideMenu{
 
#top_title, #sideMenu{
 
     display: none;
 
     display: none;
Line 23: Line 51:
 
     margin-left: 0px;
 
     margin-left: 0px;
 
}
 
}
 +
#menu li ul li:hover ul{
 +
    background:rgba(0,0,0,0.75)!important;
 +
}
 +
li#mainlevel_01 a {
 +
    color: #FFE5B5!important;
 +
 +
}
 +
li#mainlevel_01 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_02 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_02 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_03 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_03 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_05 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_05 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_06 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_06 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_07 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_07 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
li#mainlevel_08 a {
 +
    color: #FFE5B5!important;
 +
}
 +
li#mainlevel_08 a:hover {
 +
    font-size:30px!important;
 +
    text-shadow:0px 0px 8px #FFE5B5,
 +
                0px 0px 42px #FFE5B5,
 +
                0px 0px 72px #FFE5B5,
 +
                0px 0px 150px #FFE5B5;
 +
}
 +
#menu li ul li ul li a:hover {
 +
    color: rgba(0,223,252,1);
 +
    border-top: dotted 1px rgba(255,255,255,0.91);
 +
    border-bottom: dotted 1px rgba(255,255,255,0.91);
 +
    background: rgba(0,223,252,.02);
 +
}
 +
#nav .mainlevel a {
 +
    color: black;
 +
    text-decoration:none;
 +
    line-height:32px;
 +
    display:block;
 +
    padding:0 5px;
 +
    font-size: 25px!important;
 +
font-family: 'Segoe UI', Roboto, 'Helvetica Neue', Arial, sans-serif, 'Apple Color Emoji', 'Segoe UI Emoji', 'Segoe UI Symbol', 'Noto Color Emoji' !important;
 +
}
 +
.layer-bottom {
 +
z-index: -2;
 +
position: absolute;
 +
margin-top: 36px!important;
 +
}
 +
  
 
</style>
 
</style>
Line 29: Line 152:
 
<body>
 
<body>
 
<!--menu-->
 
<!--menu-->
−
<div id="menu">
+
 
−
<li id="nav">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
+
<div id="menu" style="background-color:rgba(0,0,0,0.6)!important">
−
+
<li id="nav" style="left: 8%!important; width: 100%!important;">&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;&#160;
 +
<!--<a class="menu1" href="#" style="text-align: right;">☰</a>-->
 
<ul class="firstmenu" style="float: left">
 
<ul class="firstmenu" style="float: left">
 
 
Line 47: Line 171:
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
−
  <li class="mainlevel" id="mainlevel_03">
+
−
  <a href="https://2018.igem.org/Team:NEFU_China/Basic parts"><img id="parts" src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--_PARTS.png">PARTS</a>
+
 +
<li class="mainlevel" id="mainlevel_03">
 +
  <a href="https://2018.igem.org/Team:NEFU_China/Experiment"><img id="parts" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">EXPERIMENT</a>
 
  <ul id="sub_03">
 
  <ul id="sub_03">
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic parts" target="_self">BASIC PARTS</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite parts" target="_self">COMPOSITE PARTS</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Parts collection" target="_self">PARTS COLLECTION</a></li>
 
−
  </ul>
 
−
  </li>
 
−
      <li class="mainlevel" id="mainlevel_04">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Lock_Key"><img id="results" src="https://static.igem.org/mediawiki/2018/6/62/T--NEFU_China--_RESULTS.png">RESULTS</a>
 
−
  <ul id="sub_04">
 
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Lock_Key" target="_self">LOCK &amp; KEY</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Suicide" target="_self">SUICIDE</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Splicing" target="_self">SPLICING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Demonstrate" target="_self">DEMONSTRATE</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Basic_Part" target="_self">BASIC PARTS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Composite_Part" target="_self">COMPOSITE PARTS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Improve" target="_self">IMPROVEMENT PARTS</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Part_Collection" target="_self">PARTS COLLECTION</a></li>
 +
  <hr>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">NOTEBOOK</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
 +
 
 
  <li class="mainlevel" id="mainlevel_05">
 
  <li class="mainlevel" id="mainlevel_05">
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
 
  <a href="https://2018.igem.org/Team:NEFU_China/Model"><img id="model" src="https://static.igem.org/mediawiki/2018/0/0c/T--NEFU_China--_MODEL.png">MODEL</a>
Line 76: Line 203:
 
  <ul id="sub_06">
 
  <ul id="sub_06">
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software" target="_self">OVERVIEW</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">SOFTWARE1</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software1" target="_self">CODING</a></li>
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">SOFTWARE2</a></li>
+
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software2" target="_self">MISLEADING</a></li>
 +
  <li><a href="https://2018.igem.org/Team:NEFU_China/Software3" target="_self">WORDSEGMENT</a></li>
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 85: Line 213:
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Attributions" target="_self">ATTRIBUTIONS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Members" target="_self">MEMBERS</a></li>
 +
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Sponsoring" target="_self">SPONSORING</a></li>
 
  </ul>
 
  </ul>
Line 95: Line 224:
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Collaborations" target="_self">COLLABORTION</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
 
  <li><a href="https://2018.igem.org/Team:NEFU_China/Public_Engagement" target="_self">EDUCATION &amp; PUBLIC ENGAGEMENT</a></li>
−
  </ul>
 
−
  </li>
 
−
  <li class="mainlevel" id="mainlevel_09">
 
−
  <a href="https://2018.igem.org/Team:NEFU_China/Notebook"><img id="notebook" src="https://static.igem.org/mediawiki/2018/c/cb/T--NEFU_China--_NOTEBOOK.png">NOTEBOOK</a>
 
−
  <ul id="sub_09">
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Notebook" target="_self">OVERVIRW</a></li>
 
−
  <li><a href="https://2018.igem.org/Team:NEFU_China/Protocol" target="_self">PROTOCOL</a></li>
 
 
  </ul>
 
  </ul>
 
  </li>
 
  </li>
Line 109: Line 231:
 
</div>
 
</div>
 
<div id="banner">  
 
<div id="banner">  
−
<img src="https://static.igem.org/mediawiki/2018/0/05/T--NEFU_China--DNA.png" alt="banner" id="banner-img">
+
<img src="https://static.igem.org/mediawiki/2018/f/f6/T--NEFU_China--result15.png" alt="banner" id="banner-img">
 
</div>
 
</div>
 +
<div class="layer-bottom">
 +
 +
<canvas id="canvas" style="background:#000000"></canvas>
 +
 +
<script type="text/javascript">
 +
 +
window.onload = function(){
 +
var canvas = document.getElementById("canvas");
 +
var context =canvas.getContext("2d");
 +
var W = window.innerWidth;
 +
                        var H = 9300;
 +
//var H = window.innerHeight*1.5;
 +
canvas.width = W;
 +
canvas.height = H;
 +
var fontSize = 20;
 +
var colunms = Math.floor(W /fontSize);
 +
var drops = [];
 +
for(var i=0;i<colunms;i++){
 +
drops.push(0);
 +
}
 +
 +
 +
var str1 = "ATCG";
 +
                        var str2 = "01";
 +
function draw(){
 +
context.fillStyle = "rgba(0,0,0,0.2)";
 +
context.fillRect(0,0,W,H);
 +
context.font = "700 "+fontSize+"px  微软雅黑";
 +
context.fillStyle = "#003544";
 +
for(var i=0;i<colunms/2;i++){
 +
var index = Math.floor(Math.random() * str1.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str1[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
                                for(var i=colunms/2;i<colunms;i++){
 +
var index = Math.floor(Math.random() * str2.length);
 +
var x = i*fontSize;
 +
var y = drops[i] *fontSize;
 +
context.fillText(str2[index],x,y);
 +
if(y >= canvas.height){
 +
drops[i] = 0;
 +
}
 +
                                        if(Math.random() > 0.99){
 +
                                                drops[i] = 0;
 +
}
 +
drops[i]++;
 +
}
 +
};
 +
 +
function randColor(){
 +
var r = Math.floor(Math.random() * 256);
 +
var g = Math.floor(Math.random() * 256);
 +
var b = Math.floor(Math.random() * 256);
 +
return "rgb("+r+","+g+","+b+")";
 +
}
 +
 +
draw();
 +
setInterval(draw,60);
 +
};
 +
 +
</script>
 +
 +
           
 +
 +
</div>
 +
 
<div id="background-content">
 
<div id="background-content">
−
<h1>Splicing</h1>
+
<h1 style="font-size: 65px;color: orange!important;height: 110px;"><br>
−
    <p>
+
Splicing</h1>
−
So far, the national trends in costs for wages, salaries, and benefits have glossed over these concerns. The growth in labor costs continued to slow in the second quarter - a pattern that held true in all major regions. However, the slowdown in labor costs is due solely to sharp cutbacks in what companies, mainly large corporations, are paying for benefits, which make up about a fourth of total compensation costs nationally. Because of slower growth in health care costs, workers' compensation, and state unemployment insurance, benefits grew only 2.6% during the past year, the lowest pace on record.<br>
+
−
        But since few have marked down their own prices in line with the metal's fall, they will be able to recoup much of the difference. Not so the producers, whose income is directly related to the fluctuating daily price on the London Metal Exchange.<br>
+
−
        The Japanese have their electronics, the Germans their engineering. But when it comes to command of global markets, the U.S. owns the service sector.<br>
+
−
        Meanwhile, pressure has been growing from the car companies. GM ships about 60% of its cars and trucks with Ryder, while Chrysler ships some 40%.<br>
+
−
        First of all, current modest demand growth will not support any more increases that large. Second, now that manufacturers have worked to get their inventories lower, they will be cautious about adding goods in coming months.<br>
+
−
These are two steps:
+
−
</p>
+
−
<ol>
+
−
<li>
+
−
So far, the national trends in costs for wages, salaries, and benefits have glossed over these concerns. The growth in labor costs continued to slow in the second quarter - a pattern that held true in all major regions. However, the slowdown in labor costs is due solely to sharp cutbacks in what companies, mainly large corporations, are paying for benefits, which make up about a fourth of total compensation costs nationally. Because of slower growth in health care costs, workers' compensation, and state unemployment insurance, benefits grew only 2.6% during the past year, the lowest pace on record.
+
−
</li>
+
−
<li>
+
−
So far, the national trends in costs for wages, salaries, and benefits have glossed over these concerns. The growth in labor costs continued to slow in the second quarter - a pattern that held true in all major regions. However, the slowdown in labor costs is due solely to sharp cutbacks in what companies, mainly large corporations, are paying for benefits, which make up about a fourth of total compensation costs nationally. Because of slower growth in health care costs, workers' compensation, and state unemployment insurance, benefits grew only 2.6% during the past year, the lowest pace on record.
+
−
</li>
+
−
</ol>
+
 
<p>
 
<p>
−
The Japanese have their electronics, the Germans their engineering. But when it comes to command of global markets, the U.S. owns the service sector.<br>
+
Transformation of information<br>
−
</p>
+
Every letter has its own frequency in English words, so does each codon in organisms. Therefore, we created a coding list, in which each letter corresponds to several codons with the same frequencies. In addition, we composed a program that can easily convert English sentences into DNA sequences.<br>
−
<img src="https://static.igem.org/mediawiki/2018/5/58/T--NEFU_China--Figure_1.png" alt="Figure1" id="Figure1-img">
+
We have made the transition from nucleotide sequences to DNA barcode.<br>
−
<h2 id="Figure1-title">Figure 1: This is Figure 1.</h2>
+
Information expression verification<br>
−
<p>
+
Yeast: a-type yeast.<br>
−
So far, the national trends in costs for wages, salaries, and benefits have glossed over these concerns. The growth in labor costs continued to slow in the second quarter - a pattern that held true in all major regions. However, the slowdown in labor costs is due solely to sharp cutbacks in what companies, mainly large corporations, are paying for benefits, which make up about a fourth of total compensation costs nationally. Because of slower growth in health care costs, workers' compensation, and state unemployment insurance, benefits grew only 2.6% during the past year, the lowest pace on record.<br>
+
Function: Using the specific “key-lock” interaction, information can be obtained when expressing a specific key.<br>
 +
Vector construction:<br>
 +
Gene fragments of information with a stem loop and URA screening genes were ration                                        inserted between the two homologous arms, HA1 and HA2 to construct the pesc-ura-Backbone-Stemloop-information-URA plasmid. HA1 and HA2 act as homology arms to promote the homologous recombination, which allows the integration of the DNA containing the steem loop lock sequence into the yeast genome. (配图)<br>
 +
Functional verification:<br>
 +
Plasmids with information fragments were transformed into yeast. Then we extracted the total RNA of yeast and carry out reverse transcription. DNA sequencing will be used to verify the correct expression of the information fragment is expressed, which will be compared with the original information.<br>
 
</p>
 
</p>
 
<br>
 
<br>
 +
 
<hr>
 
<hr>
 
 
 
<br>
 
<br>
−
<h1>Splicing first title</h1>
+
−
<p>
+
<br>
−
So far, the national trends in costs for wages, salaries, and benefits have glossed over these concerns. The growth in labor costs continued to slow in the second quarter - a pattern that held true in all major regions. However, the slowdown in labor costs is due solely to sharp cutbacks in what companies, mainly large corporations, are paying for benefits, which make up about a fourth of total compensation costs nationally. Because of slower growth in health care costs, workers' compensation, and state unemployment insurance, benefits grew only 2.6% during the past year, the lowest pace on record.<br>
+
−
<br>
+
−
</p>
+
−
<table id="table1">
+
−
<td valign="top">
+
−
<p>
+
−
So far, the national trends in costs for wages, salaries, and benefits have glossed over these concerns. The growth in labor costs continued to slow in the second quarter - a pattern that held true in all major regions. However, the slowdown in labor costs is due solely to sharp cutbacks in what companies, mainly large corporations, are paying for benefits, which make up about a fourth of total compensation costs nationally. Because of slower growth in health care costs, workers' compensation, and state unemployment insurance, benefits grew only 2.6% during the past year, the lowest pace on record.<br>
+
−
So far, the national trends in costs for wages, salaries, and benefits have glossed over these concerns. The growth in labor costs continued to slow in the second quarter - a pattern that held true in all major regions. However, the slowdown in labor costs is due solely to sharp cutbacks in what companies, mainly large corporations, are paying for benefits, which make up about a fourth of total compensation costs nationally. Because of slower growth in health care costs, workers' compensation, and state unemployment insurance, benefits grew only 2.6% during the past year, the lowest pace on record.<br>
+
−
</p>
+
−
</td>
+
−
<td valign="top">
+
−
<img src="https://static.igem.org/mediawiki/2018/2/2f/T--NEFU_China--Figure_2.png" alt="Figure2" id="Figure2-img">
+
−
<h2 id="Figure2-title">Figure 2: This is Figure 2.</h2>
+
−
</td>
+
−
</table>
+
−
<p>
+
−
The Japanese have their electronics, the Germans their engineering. But when it comes to command of global markets, the U.S. owns the service sector.<br>
+
−
</p>
+
−
<br><br><br>
+
 
</div>
 
</div>
 
<div id="background-reference">
 
<div id="background-reference">
−
<h1>Reference</h1>
+
<h1 style="font-size: 65px;color: orange!important;height: 70px;">Reference</h1>
 
<p> <a href="#"> [1] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>
 
<p> <a href="#"> [1] Pu, Jinyue and Zinkus-Boltz, Julia and Dickinson, Bryan C. (2017) Evolution of a split RNA polymerase as a versatile biosensor platform. <em><em>Nat Chem Biol 13</em></em> , 432-438 </a>
 
<br>
 
<br>

Revision as of 23:10, 16 October 2018

Splicing


Splicing

Transformation of information
Every letter has its own frequency in English words, so does each codon in organisms. Therefore, we created a coding list, in which each letter corresponds to several codons with the same frequencies. In addition, we composed a program that can easily convert English sentences into DNA sequences.
We have made the transition from nucleotide sequences to DNA barcode.
Information expression verification
Yeast: a-type yeast.
Function: Using the specific “key-lock” interaction, information can be obtained when expressing a specific key.
Vector construction:
Gene fragments of information with a stem loop and URA screening genes were ration inserted between the two homologous arms, HA1 and HA2 to construct the pesc-ura-Backbone-Stemloop-information-URA plasmid. HA1 and HA2 act as homology arms to promote the homologous recombination, which allows the integration of the DNA containing the steem loop lock sequence into the yeast genome. (配图)
Functional verification:
Plasmids with information fragments were transformed into yeast. Then we extracted the total RNA of yeast and carry out reverse transcription. DNA sequencing will be used to verify the correct expression of the information fragment is expressed, which will be compared with the original information.




Userfull links

Home      Model     Project    Software

Parts       Teams    Results   Notebook

Human  Practice

Follow us

facebook     twitter     wehchat
facebook     twitter     wehchat

Contact us

iGEM-NEFU_China2018

Email: hexinglu@nefu.edu.cn

No.26 Hexing Road, Xiangfang
District, Harbin, Heilongjiang
Province 150000