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Our team also dealed with searching for DNA sequences saved in the database of <a href="https://www.ncbi.nlm.nih.gov/genbank/" style="color:yellow;"> NCBI</a>. How to use the program MEGA7 and the database of NCBI is also mentioned in the Methods. | Our team also dealed with searching for DNA sequences saved in the database of <a href="https://www.ncbi.nlm.nih.gov/genbank/" style="color:yellow;"> NCBI</a>. How to use the program MEGA7 and the database of NCBI is also mentioned in the Methods. | ||
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− | Our research also required a good handling with the light- and the electron microscope. We describe in <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Pollen" style="color:yellow"> | + | Our research also required a good handling with the light- and the electron microscope. We describe in <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Pollen" style="color:yellow"> Pollen </a> how to use them. |
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One essential part of our project is the cloning of E. coli. If you want to see results and more about our cloning experiments, click <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow"> here </a>. | One essential part of our project is the cloning of E. coli. If you want to see results and more about our cloning experiments, click <a href="https://2018.igem.org/Team:Rheda_Bielefeld/Methods" style="color:yellow"> here </a>. |
Revision as of 09:27, 17 October 2018
Overview Experiments
We did two assays [1] [2] to prove the occurence of pectin; we were able to crack pollen and to clone Escherichia coli.
After cracking the pollen, we were capable to duplicate the DNA of different pollen by doing a PCR [1] [3] . Afterwards we could "inject" the duplicated DNA into a gelelectrophoresis to show which pollen we used before.
Our team also dealed with searching for DNA sequences saved in the database of NCBI. How to use the program MEGA7 and the database of NCBI is also mentioned in the Methods.
Our research also required a good handling with the light- and the electron microscope. We describe in Pollen how to use them.
One essential part of our project is the cloning of E. coli. If you want to see results and more about our cloning experiments, click here .
Pictures
Working at the assays