Collaboration with the NDC-HighRiverAB iGEM Team

We first came into contact with the NDC-HighRiverAB iGEM team at the aGEM competition in September, where we watched them present about their project "The RMS E. coli". They were working on creating E. coli that express an esterase capable of breaking down "fatbergs" that cause issues in wastewater treatment facilities. During this presentation, they indicated that they were interested in using spectrophotometry to gather quantitative data pertaining to enzyme kinetics under different reaction conditions. However, as a first year high school iGEM team, they had limited access to certain laboratory equipment.

We helped them design their spectrophotometry experiment and helped them run these assays using our spectrophotometer. They travelled from their neighbouring town of High River to our lab at the University of Calgary multiple times to attend a workshop hosted by our team's graphic design specialist and to perform the spectrophotometry experiments.

Queens Canada Collaboration

As a fellow Canadian team, we hoped for an ongoing relationship with the Queens Canada iGEM team. Coincidentally, both members of the uCalgary team and the Queens team were also participating in a second research competition called the Canada Reduced Gravity Experiment (Can-RGX). Can-RGX is a nationwide competition wherein four collegiate teams were selected to prototype and test an experiment in micro-gravity through parabolic flight. Through Can-RGX, relationships were formed very early in the year between members of both teams, which continued to pay dividends for the remainder of the summer.

Cooperative planning and troubleshooting was performed throughout various meetings, both in person and over Skype. Topics such as the acquisition of funding, the ordering of complex DNA parts, and opportunities to take advantage of were shared and debated. The Queens Canada team alerted us to a promotion offered by Genscript which provided free DNA fragment synthesis, which proved integral to our project design. They also provided advice on re-designing DNA fragments which were too problematic to synthesize as single strands, aiding us in getting the ball rolling with our wet lab research. It was rewarding and refreshing to have a cooperative team with which we shared a running conversation and friendship, even for the simple ups and downs of undergraduate research.

Queens Canada coordinator Elisha Krauss (left) and Calgary iGEM member Sam Wilton-Clark (right) meeting at the National Research Council Aviation Centre during the CAN-RGX testing phase