At first, we gave the male syncarps of birch, willow and spruce in a plastic tube, which was electrostatically loaded on its walls. During vortexing the pollen stuck to the walls and the syncarps were collected at the bottom of the container. Afterwards, the pollen were wiped from the walls with cotton buds and the cotton pellets with the pollen were vortexed. Lastly, we checked the pollen using light-microscopy if they were separated correctly according to their plant species. | At first, we gave the male syncarps of birch, willow and spruce in a plastic tube, which was electrostatically loaded on its walls. During vortexing the pollen stuck to the walls and the syncarps were collected at the bottom of the container. Afterwards, the pollen were wiped from the walls with cotton buds and the cotton pellets with the pollen were vortexed. Lastly, we checked the pollen using light-microscopy if they were separated correctly according to their plant species. |