Difference between revisions of "Team:BNU-China/Safety"

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             <h2>Overview</h2>
 
             <h2>Overview</h2>
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             <p>Our project aims to use engineered tubulin proteins to screen microtubule-stabilizing anticancer agents(anti-microtubue agents). The strain we use to express the α-C-luciferase and α-N-luciferase is <i>E.coli</i> Trans5α, <i>E.coli</i> TransB(DE3) and <i>E.coli</i> Rossetta(DE3), which are not known to be pathogenic in immunocompetent individuals. Our product proteins are also easy to be degraded in the natural environment. They work in the lab condition and can show more light when microtubule-stabilizing agents exist, for this kind of agent can conspicuously stabilize the tubulin polymerization <i>in vitro</i>. </p>
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             <p>Our project is to screen the high-producing bacteria strain based on the anti-degeneration gene circuit without antibiotics. Totally, we use three methods to achieve this goal. Respectively, we utilize the emrR gene, T7 RNA polymerase gene and CI gene as the core part of our system to screen the high-producing bacteria strain. In our circuit, only the bacteria strain which can produce more target products can stimulate the expression of the growth factor gdh. And then the high-producing bacteria strain will obtain the growth advantage. In contrast, in the low-producing bacteria, the level of the gdh gene expression is lower than that in the high-producing bacteria. Thus, the high-producing bacteria will grow faster than low-producing bacteria and the low-producing bacteria will be eliminated in the end. In this way, we successfully achieve the goal of screening the high-producing bacteria.</p>
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             <p>After a series of experiment accidents, experiment safety is now a noticeable problem in Chinese colleges. As a consequence, though our organisms and plasmids are relatively safe, we take many measures to ensure the safety of our project. <b>First</b>, we only culture the engineered strain in lab and the whole process is under strict control. This prevents the gene pollution. <b>Second</b>, we strictly follow the rules of experimental equipment we use, especially the High-Pressure Steam Sterilization Pot and the centrifuge, which take a risk to have accidents. <b>Third</b>, we follow the lab rules all the time: </p>
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            <h2>I. safety training in lab</h2>
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             <p>Before all the members of our experimental group entered the laboratory to start the experiment, all of our team members carefully read and signed the laboratory safety responsibility book. Everyone carefully understood the relevant regulations of the laboratory and ensured strict compliance with the laboratory. In the first month of the experiment, our advisor Nan conducted relevant supervision and guidance on our experiments and standardized the flow of the experimental operation. After one month, we organized a safe operation for the laboratory. The assessment is divided into two parts theory and experimental operation, only the team members who meet the requirements can be allowed to enter the laboratory to do experiments, those who fail to meet the standards will continue to receive laboratory safety education until the standard is reached.</p>
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                <li>No slippers and pants would be permitted in lab. Close­toed shoes will be worn at all times in the lab.</li>
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             <h2>II. Organism Safety</h2>
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                <li>Gloves and medical mask is ordered when working in the labs, especially with some aerosolizing agents, such as SDS.</li>
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<p>Among our entire projects, the strains we have involved are E.coli K12 Top 10, E.coli MG1655, E.coli DH5α, E.coli Stbl3, E.coli BL21 (DE3), and S.cerevisiae EBY100. The strains all belong to Risk Group 1 and Safety Level 1, and there is no harm to the human body. And our laboratory has strict management systems and regulations for laboratory waste, which can ensure that the laboratory waste in the laboratory is properly treated. And our strains can be applied to industrial production on a large scale because they are not harmful to the environment, and the safety in the production process is highly guaranteed.</p>
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                <li>Hands must be washed before leaving the lab area. And lab coats will be worn when working with bacterial cultures.</li>              
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                <li>No food or drink will be allowed in the lab.</li>
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<h3>III. experiment safety</h3>
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                <li>All bench space will be wiped down with 75% EtOH before and after use, and be exposed to UV for twenty minutes before use.</li>
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<p>In the process of our team members' experiments, we require each of our team members to strictly abide by the experimental procedures, comply with the experimental regulations, pay attention to the protection of the instruments when using the equipment, and strictly carry out the relevant instruments in accordance with relevant regulations. Maintenance, such as: centrifuge, metal bath, shaker, etc., timely repair of the faulty instrument, at the same time, pay attention to its own protection during the experiment, wear laboratory clothes, gloves, goggles and other protective equipment in strict accordance with relevant regulations, food It is forbidden to bring water into the experimental area to ensure the safety of each of our team members during the experiment.<p>
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                <li>All unnecessary equipments will be unplugged and removed from bench after use.</li>
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Revision as of 19:56, 17 October 2018

Team:BNU-CHINA - 2016.igem.org style = "font-family: Helvetica;"

Safety

Overview

Our project is to screen the high-producing bacteria strain based on the anti-degeneration gene circuit without antibiotics. Totally, we use three methods to achieve this goal. Respectively, we utilize the emrR gene, T7 RNA polymerase gene and CI gene as the core part of our system to screen the high-producing bacteria strain. In our circuit, only the bacteria strain which can produce more target products can stimulate the expression of the growth factor gdh. And then the high-producing bacteria strain will obtain the growth advantage. In contrast, in the low-producing bacteria, the level of the gdh gene expression is lower than that in the high-producing bacteria. Thus, the high-producing bacteria will grow faster than low-producing bacteria and the low-producing bacteria will be eliminated in the end. In this way, we successfully achieve the goal of screening the high-producing bacteria.

I. safety training in lab

Before all the members of our experimental group entered the laboratory to start the experiment, all of our team members carefully read and signed the laboratory safety responsibility book. Everyone carefully understood the relevant regulations of the laboratory and ensured strict compliance with the laboratory. In the first month of the experiment, our advisor Nan conducted relevant supervision and guidance on our experiments and standardized the flow of the experimental operation. After one month, we organized a safe operation for the laboratory. The assessment is divided into two parts theory and experimental operation, only the team members who meet the requirements can be allowed to enter the laboratory to do experiments, those who fail to meet the standards will continue to receive laboratory safety education until the standard is reached.

II. Organism Safety

Among our entire projects, the strains we have involved are E.coli K12 Top 10, E.coli MG1655, E.coli DH5α, E.coli Stbl3, E.coli BL21 (DE3), and S.cerevisiae EBY100. The strains all belong to Risk Group 1 and Safety Level 1, and there is no harm to the human body. And our laboratory has strict management systems and regulations for laboratory waste, which can ensure that the laboratory waste in the laboratory is properly treated. And our strains can be applied to industrial production on a large scale because they are not harmful to the environment, and the safety in the production process is highly guaranteed.

III. experiment safety

In the process of our team members' experiments, we require each of our team members to strictly abide by the experimental procedures, comply with the experimental regulations, pay attention to the protection of the instruments when using the equipment, and strictly carry out the relevant instruments in accordance with relevant regulations. Maintenance, such as: centrifuge, metal bath, shaker, etc., timely repair of the faulty instrument, at the same time, pay attention to its own protection during the experiment, wear laboratory clothes, gloves, goggles and other protective equipment in strict accordance with relevant regulations, food It is forbidden to bring water into the experimental area to ensure the safety of each of our team members during the experiment.