Difference between revisions of "Team:IIT-Madras/InterLab"

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The objective of this year’s study was to reduce lab to lab variablity in fluroscence measurements. This was done using two methods: normalising the absorbance to a known concentration of microsphere beads and counting the colony forming units. <br><br>
 
The objective of this year’s study was to reduce lab to lab variablity in fluroscence measurements. This was done using two methods: normalising the absorbance to a known concentration of microsphere beads and counting the colony forming units. <br><br>
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I Microsphere Beads Standard Curve <br><br>
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II Fluroacein Stanard Curve <br><br>
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The parts used were:<br>
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Negative control BBa_R0040 <br>
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Positive control BBa_I20270 <br>
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Test Device 1 BBa_J364000 <br>
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Test Device 2 BBa_J364001 <br>
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Test Device 3 BBa_J364002 <br>
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Test Device 4 BBa_J364007 <br>
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Test Device 5 BBa_J364008 <br>
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Test Device 6 BBa_J364009 <br>
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The study was conducted using 6 parts as listed above. <br><br>
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The parts were in the plasmid pSB1C3 which were transformed into Ecoli DH5a cells. The colonies formed were screened for fluroscence with appropriate biological and technical replicates using a plate reader and a flow cytometer. The overnight cultures were used in counting the colony forming units. <br><br>
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The 0 and 6 hour readings for two colonies that were screened per test device are depicted below.
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Revision as of 22:20, 17 October 2018


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Team: IIT-Madras/Results

Interlab

Interlab

    The objective of this year’s study was to reduce lab to lab variablity in fluroscence measurements. This was done using two methods: normalising the absorbance to a known concentration of microsphere beads and counting the colony forming units.

    I Microsphere Beads Standard Curve

    II Fluroacein Stanard Curve

    The parts used were:
    Negative control BBa_R0040
    Positive control BBa_I20270
    Test Device 1 BBa_J364000
    Test Device 2 BBa_J364001
    Test Device 3 BBa_J364002
    Test Device 4 BBa_J364007
    Test Device 5 BBa_J364008
    Test Device 6 BBa_J364009
    The study was conducted using 6 parts as listed above.

    The parts were in the plasmid pSB1C3 which were transformed into Ecoli DH5a cells. The colonies formed were screened for fluroscence with appropriate biological and technical replicates using a plate reader and a flow cytometer. The overnight cultures were used in counting the colony forming units.

    The 0 and 6 hour readings for two colonies that were screened per test device are depicted below.