Difference between revisions of "Team:IIT-Madras/Improvements"

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Improvements
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<h1 style="font-family: 'title', sans-serif; font-size: 12mm; padding-left: 20%; color: #00b355;">Integrated Human Practices</h1>
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<h2 style="font-size: 9mm;">Integrated Human Practices</h2>
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<h4 align="left">Improvements</h4>
 
Further studies that we wish to do:
 
Further studies that we wish to do:
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Using thermodynamic data and correlate it with the sequence of the promoter region. The experimental and thermodynamic data of the sequences obtained can be used to correlate it with the expression levels of the protein. Come up with a model to understand the correlation between the sequence of promoter and expression rates.  
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<ul style="font-size: 5.5mm; text-align: justify; " ALIGN=LEFT>
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Troubleshooting or try to understand the reason of extremely high expression rate of P8S.  
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Characterize remaining biobrick parts.
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<li>Using thermodynamic data and correlate it with the sequence of the promoter region. The experimental and thermodynamic data of the sequences obtained can be used to correlate it with the expression levels of the protein. Come up with a model to understand the correlation between the sequence of promoter and expression rates.</li>
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Test promoter library in other chassis such as E. coli and other gram-negative microorganisms
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<li>Troubleshooting or try to understand the reason of extremely high expression rate of P8S. </li>
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Use Codon usage table available for other organisms of industrial interest using CUTE.
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<li>Characterize remaining biobrick parts.</li>
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<li>Test promoter library in other chassis such as E. coli and other gram-negative microorganisms</li>
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<li>Use Codon usage table available for other organisms of industrial interest using CUTE.</li>
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Revision as of 02:00, 18 October 2018

iGEM Collaborations Page

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Team: IIT-Madras/Results

Integrated Human Practices

Integrated Human Practices

Improvements

Further studies that we wish to do:
  • Using thermodynamic data and correlate it with the sequence of the promoter region. The experimental and thermodynamic data of the sequences obtained can be used to correlate it with the expression levels of the protein. Come up with a model to understand the correlation between the sequence of promoter and expression rates.
  • Troubleshooting or try to understand the reason of extremely high expression rate of P8S.
  • Characterize remaining biobrick parts.
  • Test promoter library in other chassis such as E. coli and other gram-negative microorganisms
  • Use Codon usage table available for other organisms of industrial interest using CUTE.