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Revision as of 09:38, 2 November 2018

togglepH

VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 9.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced in order to repress the expression of the biobrick at high pH

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process