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Revision as of 10:02, 2 November 2018
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togglepH
VIT Vellore
We use a plasmid system consisting of a composite part with two promoters
This part gets activated at ~pH 9.5 and produces the necessary proteins to increase the surrounding pH
Repressor molecule LexA is produced in order to repress the expression of the biobrick at high pH
LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, a recA protein is produced, which binds to the repressor and prevents its action, thereby regulating the process