Difference between revisions of "Team:VIT Vellore"

 
Line 12: Line 12:
 
     </video>
 
     </video>
 
     <div class="col-12" id="home-container">
 
     <div class="col-12" id="home-container">
 +
        <img id="igem-vit-logo" src="https://static.igem.org/mediawiki/2018/d/d3/T--VIT_Vellore--iGEEM2018FINAL.png" alt="iGEM VIT"/>
 
         <h1>toggle<span class="em-text">pH</span></h1>
 
         <h1>toggle<span class="em-text">pH</span></h1>
 
         <h2>VIT Vellore</h2>
 
         <h2>VIT Vellore</h2>

Latest revision as of 04:26, 8 December 2018

togglepH

VIT Vellore

Illustration 1

We use a plasmid system consisting of a composite part with two promoters

Illustration 2

This part gets activated at ~pH 7.5 and produces the necessary proteins to increase the surrounding pH

Illustration 3

Repressor molecule LexA is produced at high pH. LexA then binds to its operator region in the initial plasmid, and represses carbonic anhydrase

Illustration 4

LexA functions as a repressor in the above figure. However, as the pH starts to become acidic again, recA protease is produced

Illustration 4

recA protease binds to LexA (repressor) and lyses it, thereby preventing its action. Carbonic anhydrase is produced again, and pH homeostasis is ensured