ATTRIBUTIONSAttributions
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Support
Our project could finish only with helps from others. We really appreciate their kindness. Without specific explanation, all the people referred are from School of Life Sciences, Jilin University.
General Support
We really appreciate Jilin University and College of Life Sciences for their support in the iGEM competition.
Thanks our instructors, Ali Hou and Yang Zhan, and advisor Jin Liu for giving us the constructive suggestions and meticulous guidance to our project.
Technique Support
Dr. Tiejun Gu taught us how to use apparatus we need in experiment.
Ph.D candidate Hongyan Xia taught us the use of the plate reader.
Material and Apparatus Support
Dr. Xinghong Zhao ordered reagents for us.
Dr. Yubin Ge provided us a plate reader.
Dr. Haifeng Tang from National Biological Experiment Teaching Demonstration Center, Jilin University, provided us a PCR instrument.
Ph.D candidate Hongyan Xia provided us Taq enzyme, dNTP mixture and 10x buffer.
Ph.D candidates Liyan Song and Hongge Wang provided us micropipettor and shaker.
Wiki Support
Our team were playing efforts to de novo synthesis of biobricks, we also believe a de novo coding is easier to construct a standardized, concise and attractive wiki. Therefore, we forced ourselves to use as less so called template materials as possible. But do to the limit time and energy, we write all codes of each elements except 2 plugins-ECharts and DataTables. Thanks to ECharts for providing vivid graphic demostrations, and DataTables for us to placed all biobricks and their attributes to our page. In addition,we'd like to thank our headquarters who helped us solved countless questions about wiki issue through e-mail!
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Team Accomplishment
Thanks for every team member's ideas in brainstorm. We designed the project together. Dr. Ali Hou, Dr. Yang Zhan and Ph.D candidate Jin Liu provide constructive suggestions to our project. Our team was responsible for all aspects of running the team. Here are some details of attribution:
Sequence Design
Xianbo Chen and Zhijie Gu designed the sequence of the CspA mRNA thermosensors, Jiangjiao Mao designed the sequence of the thermosensors based on the RNA thermometers and RNase E and Fangqi Liu designed the sequence of the thermosensors based one the RNase III.
Goldengate Assembly
Done by Jiangjiao Mao, Shuting Zheng, Haimeng Yu and Cenrong Wang.
Fluorescence Measurement
Done by Zihao Wang, Fangqi Liu, Xutong Liu, Zhijie Gu, Xianbo Chen and Yu Ma. They finished the experiments day and night.
Improvement
Done by Hetian Yuan, Ruochen Chai and Rongzhen Yu.
InterLab Study
Jiangjiao Mao performed the calibration measurement. Zihao Wang, Ruochen Chai and Hetian Yuan performed the cell measurement. Fangqi Liu and Xutong Liu performed the CFU counts.
Biobrick Construction
Hetian Yuan, Ruochen Chai and Rongzhen Yu construct the standard BioBricks.
Human Practice
Hetian Yuan, Ruochen Chai and Rongzhen Yu designed our human practice work and coordinated and planned various HP activities. Besides, all of the team member participated the activities and gained a lot.
Wiki
Done by Rongzhen Yu.
Art Design
Done by Rongzhen Yu.
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Human Practice
Acadamic Winter School
Thanks Dr.Yan Chen, Dr.Ali Hou and Dr.Yang Zhan for supporting us conduct the iGEM winter school.
Zihao Wang, Zihan Lu, Ming Han, Zhongqiao Gan, Huadong Xing, Shuai Wang, Jin Liu and Letian Bao taught cell biology, molecular biology, microbiology, genetic engineering, document retrieval, mathematical modeling and web authoring.
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Sponsor
We really appreciate JIAXING TAIMEI TECHNOLOGY for sponsoring us.
Introduction of TAIMEI:
As a leading technology solution provider in the field of life sciences, Taimei Technology is dedicated to propel biopharmaceutical industry development through state-of-the-art information technology, with focus in the field of clinical research and pharmacovigilance.
Taimei Technology provides the cloud platform for seamless clinical research collaboration between sponsors, sites, CROs, patients, regulatory agencies and third-party providers. On the foundation of our platform are 6 technology-enabled solutions encompassing data management, project management, electronic regulatory submission, central medical imaging, pharmacovigilance, and drug logistics. With biopharma industry drug development pain points in mind, Taimei Technology is dedicated to improving R&D quality and efficiency for accelerating innovation time-to-market while reducing costs.
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Other Works
Gene sequencing was done by Comate Bioscience Co. Ltd.
Gene synthesis was done by Genscript Biotechnology Co. Ltd.
The reagents we used in our lab was bought from NEB, Takara, Transgen, Tiangen, etc.