Interlab
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Bronze Medal Criterion #4
Standard Tracks: Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
For teams participating in the InterLab study, all work must be shown on this page.
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Introduction
Negative Control (BBa_R0040)
Positive Control (BBa_I20270)
Test Device 1 (BBa_J364000)
Test Device 2 (BBa_J364001)
Test Device 3 (BBa_J364002)
Test Device 4 (BBa_J364007)
Test Device 5 (BBa_J364008)
Test Device 6 (BBa_J364009)
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Device We Received
Negative Control (BBa_R0040)
Positive Control (BBa_I20270)
Test Device 1 (BBa_J364000)
Test Device 2 (BBa_J364001)
Test Device 3 (BBa_J364002)
Test Device 4 (BBa_J364007)
Test Device 5 (BBa_J364008)
Test Device 6 (BBa_J364009)
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Protocol
We strictly followed the protocol provided by iGEM. We measured the experiment of OD600 reference point and fitted the fluorescence standard curve first and then detected GFP expression in our E.coli with the plate reader Synergy HT from BioTek.
This is the transformation protocol.
This is the interlab plate reader protocol.