Team:Pasteur Paris/Notebook

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July
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Transformation of plasmids pET 43.1a and pSB1C3 in E. coli DH5-α competent cells, in order to constitute a stock of empty vectors for our further experiments.

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Bacteria transformed with pET43.1a did not grow. Bacteria transformed with pSB1C3 did grow. One colony was placed in liquid culture and we let grow overnight.

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Midiprep of bacteria containing pSB1C3.
Transformation of our first sequences from Eurofins in E. coli DH5-α:
- 3a_NGF construction Part1 (Seq#1)
- 3a_NGF construction Part2 (Seq#2)
- T7 RIP construction (Seq#8)

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All the bacteria were successfully transformed with the different plasmids. One colony from each plate was placed in liquid culture and let grow overnight.

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Midiprep of:
- Seq#1
- Seq#2
- Seq#8

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September
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October
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