Team:Pasteur Paris/Notebook
Transformation of plasmids pET 43.1a and pSB1C3 in E. coli DH5-α competent cells, in order to constitute a stock of empty vectors for our further experiments.
Bacteria transformed with pET43.1a did not grow. Bacteria transformed with pSB1C3 did grow. One colony was placed in liquid culture and we let grow overnight.
Midiprep of bacteria containing pSB1C3.
Transformation of our first sequences from Eurofins in E. coli DH5-α:
- 3a_NGF construction Part1 (Seq#1)
- 3a_NGF construction Part2 (Seq#2)
- T7 RIP construction (Seq#8)
All the bacteria were successfully transformed with the different plasmids. One colony from each plate was placed in liquid culture and let grow overnight.
Midiprep of:
- Seq#1
- Seq#2
- Seq#8
Culture of strain HB2151 transformed with pVDL 9.3 (received on 23.07.2018 from Victor de Lorenzo).
Biofilm culture on filter (0.2μm).
Transformations: To create a stock
-seq#5, seq#7 (detection) from Eurofins
-plasmids pET 43.1 and pBR322 (expression plasmids)
Biofilm culture on filter after 24 hours incubation did grow. Bacteria transformed with pET43.1a did not grow. Overnight liquid culture of seq#5, seq#7 and plasmid pBR322.
Midiprep of plasmid pVDL 9.3 and measure DNA concentration with nanodrop.
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