Team:Grenoble-Alpes/construction

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PROBE CONSTRUCTION

PSEUDOMONAS AERUGINOSA DETECTION

LYSIS

1. Plasmid backbone selection

Among all the backbones proposed by iGEM, we decided to choose one for our probe insertion. In fact, the 2017 IGEM Grenoble team showed by their experiments, that the pSB1C3-BBa_J04450 backbone was the most suitable for the detection of a red fluorescence.


Figure 1: Backbone tested for hybridization of the target to the probe detecting bacterial lysis