1. Resuspend 2 nmol de la library pool on 200 µl of Binding Buffer (BB).
2. Denatured the library by heating it at 90ºC for 10 min and immediately cold it on ice for another 10 min.
3. To get rid of the DNA that unespecifically binds to the system, apply the library through a nitrocellulose membrane and centrifuge 1 min at 8000 rpm. Quantify the DNA that does not bind unspeficically and note it as the initial DNA. .
