Aptamer's Protocols
Texto de explicacion/ resumen de la pagina.
Aptamer Characterization
Elona
Aptamer electrode
Sinthesis of the electrode
SELEX
Bill Of Materials: You could see a complete BoM here (upload the bill).
Amount of time: 2 day
Total costs: calcular con BoM
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We choose Dropsens as our provider, because they are one of the standards in the field, and they are relatively near to our laboratory
The material of the working electrode was choose as carbon, modified to include gold nanoparticles. The carbon have better electrochemical window than gold or silver (check this post for more information) and gold are the ideal substrate to join DNA (It only have to be thiolated).
Selecting the electrode
There are so many scaffolds to join the Aptamers (or the DNA). Our choice was based on the kind of measuring hardware that we have used, a potentiostat. For this variety of measuring system you need a 3-electrodes system (working, reference and counter electrodes). The other parameters of the electrode was choose as follows