Team:Jilin China/Result/Version 3
VERSION 3.0
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Results
We added 10 cold-reprssible RNA-based thermosensors and 8 cold-inducible RNA-based thermosensors to the SynRT Toolkit. After that, we named the updated toolkit as SynRT Toolkit version 3.0.
Cold-repressible RNA-based thermosensors
Cold-repressible RNA-based thermosensors are designed based on the RNase III. We constructed their measurement devices and measured the activities at five temperatures: 15, 25, 29, 35 and 37℃. The measured temperature range is lower than the heat-inducible RNA-based thermosensors' temperature range. We also designed a new negtive control, which always has a cleavage site of RNase III, so it will be digest by the enzyme. After measurement, we chose 10 out of 50 thermosensors.
Figure 1 shows the change of activities of different thermosensors. We find that all of these thermosensors' activities increase with temperature increasing.
For most of the cold-repressible RNA-based thermosensors, the change of activities from 15 to 25℃ is noticeable than 25 to 35℃. And the melting temperature is lower than the heat-inducible RNA thermosensors. Figure 2 shows the fold-change of activity in different temperature.