Bronze medal criteria

Explanation	Criteria achieved
Registration and Giant Jamboree Attendance	We registered for iGEM then had a great summer time. We are now waiting to attend the Giant Jamboree.
Competition Deliverables	We plan to make the competition deliverables met on time.
Attributions	We completed the attributions page on our wiki.
Characterization / Contribution	We successfully completed the InterLab Measurement Study.

Silver medal criteria

Explanation	Criteria achieved
Validated Part / Validated Contribution	We created a new BioBrick part as expected : BBa_K2616000. This part permits to secrete NGF directly in the extracellular medium using E. coli type I secretion system. We used inducible promoter T7, in order to control NGF production thanks to IPTG induction. We also added an His-tag in N-terminal in order to purify it. NGF is adressed to Type I Secretion System by fusing to it the the 60 C-terminal aminoacide of alpha-haemolysin HlyA. Since the export peptide is not processed when passing through the secretion pore, we separated NGF from this 60 aminoacid long sequence by the cleaving site for TEV protease ENLYFQ. This part also permits to secrete TEV protease, under the same promoter, via this same secretion system.
Collaboration	We hosted the 4th Parisian Meet-Up where we organised a Tiny Jamboree in order to practice with all the French teams for the Giant. We also attended round tables about bioethics. We collaborated with other teams, you can read about them on this page.
Human Practices	Ethics and safety: Security: The Institut Pasteur is a highly restricted area so we had ID badges to enter every building and unity. + substances we used Sustainability: ? Kill switch ? Social justice: ? Intellectual property rights: account of state of the art, no infrigement of IP laws.

Explanation	Criteria achieved
Integrated Human Practices	Integrated HP
Improve a Previous Part or Project
Model Your Project	We modelled how NGF is produced in our modified E. Coli, its diffusion in an environment and its consequences on neurons growth. It helped to predict how to use NGF in our experiments.