Team:IIT-Madras/Description

Project

Description Design Notebook Results Demonstrations Improvements InterLab Safety

Parts Software

Human Practices

Overview Silver Human Practices Integrated Human Practices

Public Engagement

Overview The Language Project School Visits Research Magazine Workshop SynBio Journal Club

The Team

The Team Attributions Collaborations

× Home Project

Description Design Notebook Results Demonstrations Improvements InterLab Safety

Parts Software Human Practices

Overview Silver Human Practices Integrated Human Practices

Public Engagement

Overview Language Project School Visits Research Magazine Workshop SynBio Journal Club

The Team

The Team Attributions Collaborations

☰

Description

ADaPtat1on

Motivations:

1. Initial Idea of lignin degradation to produce higher valued vanillin. We look up the literature and we look at nature that can degrade the aromatic rings of Lignin. We needed an organism that could degrade aromatics.
2. For popular organism like E.coli and L. lactis., we need to make competent cells for transformation. We looked up into literature and nature and found many ‘Naturally Competent’ microorganisms. However, most of them are pathogenic and known to cause harm to humans. Hence, you cannot work with it in Biosafety level 1 lab. For example: Streptococcus pneumoniae (Griffith's "pneumococcus"), Neisseria gonorrhoeae, Bacillus subtilis and Haemophilus influenzae.

Solution:

The organism that best fits into both requirements was Acinetobacter baylyi ADP1 strain. This organism is naturally competent, nonpathogenic, has amazing property of degrading aromatics. (the strain can be purchased from DSMZ.)

Motivation:

We looked up the literature for Acinetobacter baylyi ADP1 but could not find substantial literature backing. The literature was too less and not many tools were available to do synthetic biology experiments with this organism. So, This was the motivation to generate a toolbox for Acinetobacter baylyi and try to make it as generic as possible so that if any other team or research groups wish to work with other organism then they can used these tools.