Team:Madrid-OLM/Hardware

• Team
  • Members
  • Attribution
  • Sponsors
• Project
  • Description
  • Contribution
  • Improve
  • Demonstrate
  • Judging Form
• Aptamers
  • Overview
  • Discovery
  • Characterization
  • Computational
    Improvement
  • Binding to Electrode
• Device
  • Overview
  • First Prototype
  • Final Prototype
    • Overview
    • Microfluidics
    • Electronics
• Experiments
  • Interlab
  • Protocols
    • Aptamer Related
    • Manufacturing
• Human Practices
  • Integrated Human Practice
  • Education and
    Engagements
  • Collaborations
• Awards
  • Hardware
  • Applied Design
  • Entrepreneurship
  • Measurement
• Medals

Final device

Our second device was born from a need. It was not preconceived, as the first prototype. We needed to solve many experimental inconveniences and many misconceptions that we had when we designed the first prototype.

First Prototype

The environment that motivated the birth of the final prototype was a different one. We would love to consider this device as a mature version of the initial one. We introduced the following improvements:

1. Arduino’s M7 diode, which job is to avoid an eventual situation of reverse current, has been removed. This is because of his inability to stand the 4 amperes that go through the system when the 8 motors are at their full capacity..

In its position, we have solder a IRLZ44N transistor, able to stand up to 50 A. To do it, the pins of the source and drain were connected in a similar way as the pins of the diode and the gate pin was connected to the 12V power supply. A heat sink was also put in the upper side.

2. An Arduino Shield was mounted to increase the total of pins to 8 Vin an 8 GND, to connects the power of the motor drivers.

The results demonstrate the capacity of our electrode to change the voltage proportionally to the bound thrombin. We could establish a mathematical model which correlates the voltage drop (compared to a negative control) to the thrombin concentration in the sample.